Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae)
Honeydew produced by insects is not only recognized as a nutrient-rich substance for natural enemies, influencing their survival, development, and reproduction but also a source of volatiles that regulate the foraging behavior of natural enemies. Hippodamia variegata (Coleoptera: Coccinellidae) is a...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000386 |
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| author | Panjing Liu Tao Zhang Yu Gao Xiaofang Zhang Hongyi Wei Yanhui Lu |
| author_facet | Panjing Liu Tao Zhang Yu Gao Xiaofang Zhang Hongyi Wei Yanhui Lu |
| author_sort | Panjing Liu |
| collection | DOAJ |
| description | Honeydew produced by insects is not only recognized as a nutrient-rich substance for natural enemies, influencing their survival, development, and reproduction but also a source of volatiles that regulate the foraging behavior of natural enemies. Hippodamia variegata (Coleoptera: Coccinellidae) is an effective predatory natural enemy of Aphis gossypii (Hemiptera: Aphididae) in Xinjiang, China. However, the functional significance of honeydew volatiles from A. gossypii for H. variegata remains largely unclear. Here we reported that H. variegata adults were attracted by the crude honeydew of A. gossypii, and we identified fifteen volatiles using headspace solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS). Electroantennogram (EAG) results showed that H. variegata exhibits a significant dose-dependent response to nine volatiles from honeydew. Y-tube olfactometer and cage predation tests showed that 1-heptanol, 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol attracted H. variegata adults. Furthermore, our study also elucidated the relationship between honeydew compounds and microorganisms. The findings demonstrated that the compounds 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol originate from five distinct cultivable bacteria identified using the 16S rRNA markers. Our results provide potential eco-friendly strategies for enhancing biological control of aphids. |
| format | Article |
| id | doaj-art-4dd04ba92e29401f96ef8c97aafe185a |
| institution | OA Journals |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-4dd04ba92e29401f96ef8c97aafe185a2025-08-20T02:02:05ZengElsevierBiological Control1049-96442025-03-0120210572810.1016/j.biocontrol.2025.105728Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae)Panjing Liu0Tao Zhang1Yu Gao2Xiaofang Zhang3Hongyi Wei4Yanhui Lu5Ministry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China; Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei Province, Baoding 071000, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei Province, Baoding 071000, ChinaMinistry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei Province, Baoding 071000, ChinaMinistry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Corresponding authors.Honeydew produced by insects is not only recognized as a nutrient-rich substance for natural enemies, influencing their survival, development, and reproduction but also a source of volatiles that regulate the foraging behavior of natural enemies. Hippodamia variegata (Coleoptera: Coccinellidae) is an effective predatory natural enemy of Aphis gossypii (Hemiptera: Aphididae) in Xinjiang, China. However, the functional significance of honeydew volatiles from A. gossypii for H. variegata remains largely unclear. Here we reported that H. variegata adults were attracted by the crude honeydew of A. gossypii, and we identified fifteen volatiles using headspace solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS). Electroantennogram (EAG) results showed that H. variegata exhibits a significant dose-dependent response to nine volatiles from honeydew. Y-tube olfactometer and cage predation tests showed that 1-heptanol, 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol attracted H. variegata adults. Furthermore, our study also elucidated the relationship between honeydew compounds and microorganisms. The findings demonstrated that the compounds 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol originate from five distinct cultivable bacteria identified using the 16S rRNA markers. Our results provide potential eco-friendly strategies for enhancing biological control of aphids.http://www.sciencedirect.com/science/article/pii/S1049964425000386Hippodamia variegataAphid HoneydewOlfactory ResponsesPredationBacteria |
| spellingShingle | Panjing Liu Tao Zhang Yu Gao Xiaofang Zhang Hongyi Wei Yanhui Lu Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) Biological Control Hippodamia variegata Aphid Honeydew Olfactory Responses Predation Bacteria |
| title | Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) |
| title_full | Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) |
| title_fullStr | Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) |
| title_full_unstemmed | Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) |
| title_short | Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae) |
| title_sort | volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by hippodamia variegata coleoptera coccinellidae |
| topic | Hippodamia variegata Aphid Honeydew Olfactory Responses Predation Bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000386 |
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