Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3

The discharge of organic and inorganic nitrogenous pollutants in wastewater leads to eutrophication and disrupts the ecological balance. Therefore, the pressing need for an effective treatment method has become increasingly evident. A robust bacterium <i>Pseudomonas stutzeri</i> H3 capab...

Full description

Saved in:
Bibliographic Details
Main Authors: Jie Hu, Bing Xu, Jiabao Yan, Guozhi Fan
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/13/3/687
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850091565673873408
author Jie Hu
Bing Xu
Jiabao Yan
Guozhi Fan
author_facet Jie Hu
Bing Xu
Jiabao Yan
Guozhi Fan
author_sort Jie Hu
collection DOAJ
description The discharge of organic and inorganic nitrogenous pollutants in wastewater leads to eutrophication and disrupts the ecological balance. Therefore, the pressing need for an effective treatment method has become increasingly evident. A robust bacterium <i>Pseudomonas stutzeri</i> H3 capable of simultaneous organic and inorganic nitrogen removal was isolated from the activated sludge in the coking wastewater treatment system. The optimal conditions for the simultaneous removal of ammonium nitrogen and quinoline were as follows: C/N ratio of 15–20, initial pH of 7–8, culture temperature of 30 °C, and shaking speed of 150–300 rpm. At 200 mg/L ammonium nitrogen and 100 mg/L quinoline, strain H3 achieved above 90% of removal efficiency, exhibiting excellent simultaneous nitrogen removal capabilities. The outstanding nitrogen removal efficiencies in the presence of quinoline and different inorganic nitrogen sources further confirmed the simultaneous organic and inorganic nitrogen removal capability of strain H3. The whole genome sequencing and nitrogen metabolic intermediates determination of strain H3 were performed to elucidate the gene function annotations, nitrogen removal function genes, and nitrogen metabolic pathways. The findings provide a promising pathway to treat the organic and inorganic nitrogenous pollutants in wastewater.
format Article
id doaj-art-4dc67c82fc3f4a7f96e4da50be46c48d
institution DOAJ
issn 2076-2607
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Microorganisms
spelling doaj-art-4dc67c82fc3f4a7f96e4da50be46c48d2025-08-20T02:42:22ZengMDPI AGMicroorganisms2076-26072025-03-0113368710.3390/microorganisms13030687Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3Jie Hu0Bing Xu1Jiabao Yan2Guozhi Fan3School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaSchool of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Province Key Laboratory of Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, ChinaSchool of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaThe discharge of organic and inorganic nitrogenous pollutants in wastewater leads to eutrophication and disrupts the ecological balance. Therefore, the pressing need for an effective treatment method has become increasingly evident. A robust bacterium <i>Pseudomonas stutzeri</i> H3 capable of simultaneous organic and inorganic nitrogen removal was isolated from the activated sludge in the coking wastewater treatment system. The optimal conditions for the simultaneous removal of ammonium nitrogen and quinoline were as follows: C/N ratio of 15–20, initial pH of 7–8, culture temperature of 30 °C, and shaking speed of 150–300 rpm. At 200 mg/L ammonium nitrogen and 100 mg/L quinoline, strain H3 achieved above 90% of removal efficiency, exhibiting excellent simultaneous nitrogen removal capabilities. The outstanding nitrogen removal efficiencies in the presence of quinoline and different inorganic nitrogen sources further confirmed the simultaneous organic and inorganic nitrogen removal capability of strain H3. The whole genome sequencing and nitrogen metabolic intermediates determination of strain H3 were performed to elucidate the gene function annotations, nitrogen removal function genes, and nitrogen metabolic pathways. The findings provide a promising pathway to treat the organic and inorganic nitrogenous pollutants in wastewater.https://www.mdpi.com/2076-2607/13/3/687simultaneous nitrogen removalorganic nitrogeninorganic nitrogennitrogen metabolic pathwaywhole genome sequencing
spellingShingle Jie Hu
Bing Xu
Jiabao Yan
Guozhi Fan
Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
Microorganisms
simultaneous nitrogen removal
organic nitrogen
inorganic nitrogen
nitrogen metabolic pathway
whole genome sequencing
title Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
title_full Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
title_fullStr Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
title_full_unstemmed Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
title_short Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium <i>Pseudomonas stutzeri</i> H3
title_sort characteristics and mechanisms of simultaneous quinoline and ammonium nitrogen removal by a robust bacterium i pseudomonas stutzeri i h3
topic simultaneous nitrogen removal
organic nitrogen
inorganic nitrogen
nitrogen metabolic pathway
whole genome sequencing
url https://www.mdpi.com/2076-2607/13/3/687
work_keys_str_mv AT jiehu characteristicsandmechanismsofsimultaneousquinolineandammoniumnitrogenremovalbyarobustbacteriumipseudomonasstutzeriih3
AT bingxu characteristicsandmechanismsofsimultaneousquinolineandammoniumnitrogenremovalbyarobustbacteriumipseudomonasstutzeriih3
AT jiabaoyan characteristicsandmechanismsofsimultaneousquinolineandammoniumnitrogenremovalbyarobustbacteriumipseudomonasstutzeriih3
AT guozhifan characteristicsandmechanismsofsimultaneousquinolineandammoniumnitrogenremovalbyarobustbacteriumipseudomonasstutzeriih3