Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems

Abstract Common bean is an important component of global nutritional security. Climate change driven water deficit stress impairs crop performance by implicating both above and below ground plant parts in crops like common beans that are largely grown in marginal low input farming systems. In order...

Full description

Saved in:
Bibliographic Details
Main Authors: Ishrat Riyaz, Aaqif Zaffar, Samreen Fatima, Sadiah Shafi, Rayan Bhat, Sanifa Showkat, Tamana Khan, Fehim J. Wani, Sajad Majeed Zargar, P. V. Vara Prasad, Parvaze A. Sofi
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-04635-8
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849724966817234944
author Ishrat Riyaz
Aaqif Zaffar
Samreen Fatima
Sadiah Shafi
Rayan Bhat
Sanifa Showkat
Tamana Khan
Fehim J. Wani
Sajad Majeed Zargar
P. V. Vara Prasad
Parvaze A. Sofi
author_facet Ishrat Riyaz
Aaqif Zaffar
Samreen Fatima
Sadiah Shafi
Rayan Bhat
Sanifa Showkat
Tamana Khan
Fehim J. Wani
Sajad Majeed Zargar
P. V. Vara Prasad
Parvaze A. Sofi
author_sort Ishrat Riyaz
collection DOAJ
description Abstract Common bean is an important component of global nutritional security. Climate change driven water deficit stress impairs crop performance by implicating both above and below ground plant parts in crops like common beans that are largely grown in marginal low input farming systems. In order to develop climate resilient bean cultivars, it is imperative to understand response of root and shoot traits to water deficit stress and identify genotypes with adaptive plasticity under stress. In the present study, we assessed the differential response of 45 bean genotypes for root and shoot traits under agar system, PEG-6000 mediated in vitro stress as well as column culture in greenhouse. There was significant genetic variability in per se response and plasticity of root and shoot traits, under control and water deficit stress, Basal root angle ranged from 36.67 to 56.67 while as basal root number had a range of 7.45–14.33. Severe reduction in root and shoot traits was observed under water deficit stress in shoot biomass (60.20%), followed by plant height (42.40%), root biomass (31.50%), while as lowest decrease was observed in rooting depth (13.33%). However, root-shoot ratio increased by 89.05% under water deficit stress. Pearson correlation and PCA revealed that root depth and root biomass significantly impact plant height, shoot biomass and number of leaves further reaffirming our hypothesis that roots traits are important selection attributes for above ground plant performance under stress conditions. Genotypes WB-216 and N-2 were superior for most of the traits with adaptive plasticity response to water deficit stress and can be used for development of climate resilient bean varieties.
format Article
id doaj-art-4dc3d81a3ed5498599cc0e2b9b47cfcb
institution DOAJ
issn 2045-2322
language English
publishDate 2025-06-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-4dc3d81a3ed5498599cc0e2b9b47cfcb2025-08-20T03:10:35ZengNature PortfolioScientific Reports2045-23222025-06-0115111310.1038/s41598-025-04635-8Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systemsIshrat Riyaz0Aaqif Zaffar1Samreen Fatima2Sadiah Shafi3Rayan Bhat4Sanifa Showkat5Tamana Khan6Fehim J. Wani7Sajad Majeed Zargar8P. V. Vara Prasad9Parvaze A. Sofi10Stress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirDivision of Plant Biotechnology, SKUAST-KashmirDepartment of Agronomy, Kansas State UniversityStress Physiology Lab, Division of Genetics and Plant Breeding, SKUAST-KashmirAbstract Common bean is an important component of global nutritional security. Climate change driven water deficit stress impairs crop performance by implicating both above and below ground plant parts in crops like common beans that are largely grown in marginal low input farming systems. In order to develop climate resilient bean cultivars, it is imperative to understand response of root and shoot traits to water deficit stress and identify genotypes with adaptive plasticity under stress. In the present study, we assessed the differential response of 45 bean genotypes for root and shoot traits under agar system, PEG-6000 mediated in vitro stress as well as column culture in greenhouse. There was significant genetic variability in per se response and plasticity of root and shoot traits, under control and water deficit stress, Basal root angle ranged from 36.67 to 56.67 while as basal root number had a range of 7.45–14.33. Severe reduction in root and shoot traits was observed under water deficit stress in shoot biomass (60.20%), followed by plant height (42.40%), root biomass (31.50%), while as lowest decrease was observed in rooting depth (13.33%). However, root-shoot ratio increased by 89.05% under water deficit stress. Pearson correlation and PCA revealed that root depth and root biomass significantly impact plant height, shoot biomass and number of leaves further reaffirming our hypothesis that roots traits are important selection attributes for above ground plant performance under stress conditions. Genotypes WB-216 and N-2 were superior for most of the traits with adaptive plasticity response to water deficit stress and can be used for development of climate resilient bean varieties.https://doi.org/10.1038/s41598-025-04635-8Common beanWater deficit stressRoot architectureBasal root angle
spellingShingle Ishrat Riyaz
Aaqif Zaffar
Samreen Fatima
Sadiah Shafi
Rayan Bhat
Sanifa Showkat
Tamana Khan
Fehim J. Wani
Sajad Majeed Zargar
P. V. Vara Prasad
Parvaze A. Sofi
Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
Scientific Reports
Common bean
Water deficit stress
Root architecture
Basal root angle
title Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
title_full Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
title_fullStr Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
title_full_unstemmed Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
title_short Identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
title_sort identification of water deficit stress tolerant genotypes of common bean using adaptive root and shoot traits under different screening systems
topic Common bean
Water deficit stress
Root architecture
Basal root angle
url https://doi.org/10.1038/s41598-025-04635-8
work_keys_str_mv AT ishratriyaz identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT aaqifzaffar identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT samreenfatima identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT sadiahshafi identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT rayanbhat identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT sanifashowkat identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT tamanakhan identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT fehimjwani identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT sajadmajeedzargar identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT pvvaraprasad identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems
AT parvazeasofi identificationofwaterdeficitstresstolerantgenotypesofcommonbeanusingadaptiverootandshoottraitsunderdifferentscreeningsystems