ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters
This study aimed to evaluate proAKAP4concentrations in canine fresh semen and to determine their correlation with sperm motility and fertility parameters. For this purpose, 26 ejaculates from 18 dogs were analyzed. Motility and kinetic parameters of the sperm-rich fractions were assessed by a comput...
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Elsevier
2025-06-01
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| Series: | Veterinary and Animal Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2451943X25000316 |
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| author | Djemil Bencharif Rédha Belala Nora Mimoune Dolorès Le Couazer Hamed Farnia |
| author_facet | Djemil Bencharif Rédha Belala Nora Mimoune Dolorès Le Couazer Hamed Farnia |
| author_sort | Djemil Bencharif |
| collection | DOAJ |
| description | This study aimed to evaluate proAKAP4concentrations in canine fresh semen and to determine their correlation with sperm motility and fertility parameters. For this purpose, 26 ejaculates from 18 dogs were analyzed. Motility and kinetic parameters of the sperm-rich fractions were assessed by a computer assisted semen analyzer (Hamilton Thorne HT-IVOS II, USA). ProAKAP4 concentrations were measured (containing either the spermatic, urethral, or prostatic fraction) using the Dog 4MID® kit (4BioDx, France). In the spermatic fractions, on average, the proAKAP4 concentration was 17.08 ng/106spermatozoid (spz) with a total motility of 75.92 % and progressive motility of 58.08 %. Spearman test showed a positive correlation between proAKAP4 concentrations and the total motility (r = 0.584; p = 0.08), progressive motility (r = 0.6336; p = 0.0005), and the velocity parameters: VAP (r = 0.4178; p = 0.0337), VSL (r = 0.3919; p = 0.0477) and mainly VCL (r = 0.5266; p = 0.0057). For fertility monitoring, 5 ejaculates were used to perform artificial insemination (AI). The mean proAKAP4 concentration was 25.91 ng/106spz with an average total motility of 74.4 % and progressive motility of 53 %. Four female dogs were pregnant with litter sizes ranging from 4 to 11 puppies. To conclude, this is the first study presenting the significant correlation between proAKAP4 concentration and sperm motility parameters in dogs. Further investigations with a large number of animals are recommended to test the correlation between proAKAP4 and fertility parameters. |
| format | Article |
| id | doaj-art-4dbba66cab174d2e9293fbfebccec1e6 |
| institution | OA Journals |
| issn | 2451-943X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Veterinary and Animal Science |
| spelling | doaj-art-4dbba66cab174d2e9293fbfebccec1e62025-08-20T01:51:45ZengElsevierVeterinary and Animal Science2451-943X2025-06-012810045510.1016/j.vas.2025.100455ProAKAP4 concentration in fresh canine semen and its correlation with motility parametersDjemil Bencharif0Rédha Belala1Nora Mimoune2Dolorès Le Couazer3Hamed Farnia4Department of Biotechnology and Pathology of Reproduction, ONIRIS, Nantes, France; INSERM, Nantes Université, CHU Nantes, Center for Research in Transplantation and Translational Immunology, UMR 1064, F-44000 Nantes, France; Corresponding author.Biotechnologies Laboratory Related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria; Biotechnologies Platform for Animal Medicine and Reproduction (BIOMERA), Saad Dahleb Blida University 1, AlgeriaBiotechnologies Laboratory Related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria; Biotechnologies Platform for Animal Medicine and Reproduction (BIOMERA), Saad Dahleb Blida University 1, Algeria; Higher National Veterinary School, Algiers, AlgeriaDepartment of Biotechnology and Pathology of Reproduction, ONIRIS, Nantes, FranceDepartment of Biotechnology and Pathology of Reproduction, ONIRIS, Nantes, FranceThis study aimed to evaluate proAKAP4concentrations in canine fresh semen and to determine their correlation with sperm motility and fertility parameters. For this purpose, 26 ejaculates from 18 dogs were analyzed. Motility and kinetic parameters of the sperm-rich fractions were assessed by a computer assisted semen analyzer (Hamilton Thorne HT-IVOS II, USA). ProAKAP4 concentrations were measured (containing either the spermatic, urethral, or prostatic fraction) using the Dog 4MID® kit (4BioDx, France). In the spermatic fractions, on average, the proAKAP4 concentration was 17.08 ng/106spermatozoid (spz) with a total motility of 75.92 % and progressive motility of 58.08 %. Spearman test showed a positive correlation between proAKAP4 concentrations and the total motility (r = 0.584; p = 0.08), progressive motility (r = 0.6336; p = 0.0005), and the velocity parameters: VAP (r = 0.4178; p = 0.0337), VSL (r = 0.3919; p = 0.0477) and mainly VCL (r = 0.5266; p = 0.0057). For fertility monitoring, 5 ejaculates were used to perform artificial insemination (AI). The mean proAKAP4 concentration was 25.91 ng/106spz with an average total motility of 74.4 % and progressive motility of 53 %. Four female dogs were pregnant with litter sizes ranging from 4 to 11 puppies. To conclude, this is the first study presenting the significant correlation between proAKAP4 concentration and sperm motility parameters in dogs. Further investigations with a large number of animals are recommended to test the correlation between proAKAP4 and fertility parameters.http://www.sciencedirect.com/science/article/pii/S2451943X25000316AKAP4 precursorDogSpermCASA motilityCorrelation |
| spellingShingle | Djemil Bencharif Rédha Belala Nora Mimoune Dolorès Le Couazer Hamed Farnia ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters Veterinary and Animal Science AKAP4 precursor Dog Sperm CASA motility Correlation |
| title | ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters |
| title_full | ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters |
| title_fullStr | ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters |
| title_full_unstemmed | ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters |
| title_short | ProAKAP4 concentration in fresh canine semen and its correlation with motility parameters |
| title_sort | proakap4 concentration in fresh canine semen and its correlation with motility parameters |
| topic | AKAP4 precursor Dog Sperm CASA motility Correlation |
| url | http://www.sciencedirect.com/science/article/pii/S2451943X25000316 |
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