Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase
The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elutio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-05-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1177/0263617418760637 |
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| author | Rüstem Keçili Umut Çelikoğlu Sevgi Mil Arzu Ersöz Rıdvan Say |
| author_facet | Rüstem Keçili Umut Çelikoğlu Sevgi Mil Arzu Ersöz Rıdvan Say |
| author_sort | Rüstem Keçili |
| collection | DOAJ |
| description | The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g −1 cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out. |
| format | Article |
| id | doaj-art-4db4c4a4e420471ab96a09bff627ad1d |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-4db4c4a4e420471ab96a09bff627ad1d2025-01-03T01:19:32ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382018-05-013610.1177/0263617418760637Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidaseRüstem KeçiliUmut ÇelikoğluSevgi MilArzu ErsözRıdvan SayThe present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g −1 cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out.https://doi.org/10.1177/0263617418760637 |
| spellingShingle | Rüstem Keçili Umut Çelikoğlu Sevgi Mil Arzu Ersöz Rıdvan Say Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase Adsorption Science & Technology |
| title | Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase |
| title_full | Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase |
| title_fullStr | Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase |
| title_full_unstemmed | Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase |
| title_short | Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase |
| title_sort | concanavalin a photocross linked affinity cryogels for the purification of horseradish peroxidase |
| url | https://doi.org/10.1177/0263617418760637 |
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