Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD
Abstract Objective To investigate the clinical value and mechanism of action of long non-coding RNA PRKCQ-AS1 for lung adenocarcinoma (LUAD) progression. Methods Clinical data of 128 LUAD patients were collected, postoperative pathological tissues were stored at -80 °C. Kaplan-Meier survival analysi...
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BMC
2025-07-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00482-9 |
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| author | Lingling Liu Xiaofen Liu Xiaojiao Wu Hang Fang Jingjing Shi Wei Jiang |
| author_facet | Lingling Liu Xiaofen Liu Xiaojiao Wu Hang Fang Jingjing Shi Wei Jiang |
| author_sort | Lingling Liu |
| collection | DOAJ |
| description | Abstract Objective To investigate the clinical value and mechanism of action of long non-coding RNA PRKCQ-AS1 for lung adenocarcinoma (LUAD) progression. Methods Clinical data of 128 LUAD patients were collected, postoperative pathological tissues were stored at -80 °C. Kaplan-Meier survival analysis was employed to investigate differences in 5-year survival rates across various expression groups, while Cox regression models assessed the prognostic factors influencing patient outcomes. Reverse transcription quantitative PCR (RT-qPCR) was utilized to measure the expression levels of PRKCQ-AS1 and miR-582-3p in pathological tissues and LUAD cell lines. Additionally, a dual-luciferase reporter assay validated the reciprocal relationship. CCK8 examined cell proliferation, Transwell observed cell migration and invasion. Results PRKCQ-AS1 was down-regulated and miR-582-3p was up-regulated in LUAD tissues and cell. PRKCQ-AS1 and miR-582-3p expression affects some pathological features (lymph node metastasis, TNM stage, tumour differentiation) in LUAD patients. Patients with low PRKCQ-AS1 and high miR-582-3p had increased mortality. Interaction of PRKCQ-AS1 targeting miR-582-3p exists in LUAD cells. RGMB, STXBP6 are downstream target genes of miR-582-3p. Overexpression of (oe-) PRKCQ-AS1 inhibited LUAD cell proliferation, migration, and invasion. However, concomitant use of miR-582-3p mimics resisted the effects of PRKCQ-AS1 overexpression on cells. Conclusion PRKCQ-AS1/miR-582-3p axis regulatory relationship exists in lung adenocarcinoma cells. PRKCQ-AS1 may regulate the proliferation, migration and invasion of lung adenocarcinoma cells and participate in LUAD regulation by targeting miR-582-3p. Clinical trial number Not applicable. |
| format | Article |
| id | doaj-art-4da8e51d5bba4776aae7fa992af4efa4 |
| institution | DOAJ |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-4da8e51d5bba4776aae7fa992af4efa42025-08-20T03:03:37ZengBMCHereditas1601-52232025-07-01162111210.1186/s41065-025-00482-9Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUADLingling Liu0Xiaofen Liu1Xiaojiao Wu2Hang Fang3Jingjing Shi4Wei Jiang5Integrated Chinese and Western Medicine Oncology, the First Hospital of QiqiharDepartment of Respiratory and Critical Care Medicine, Taikang Tongji (Wuhan) HospitalDepartment of Laboratory, The First People’s Hospital of YongkangDepartment of Laboratory, The First People’s Hospital of YongkangDepartment of Laboratory, The First People’s Hospital of YongkangDepartment of Respiratory Medicine, Yantai Yuhuangding HospitalAbstract Objective To investigate the clinical value and mechanism of action of long non-coding RNA PRKCQ-AS1 for lung adenocarcinoma (LUAD) progression. Methods Clinical data of 128 LUAD patients were collected, postoperative pathological tissues were stored at -80 °C. Kaplan-Meier survival analysis was employed to investigate differences in 5-year survival rates across various expression groups, while Cox regression models assessed the prognostic factors influencing patient outcomes. Reverse transcription quantitative PCR (RT-qPCR) was utilized to measure the expression levels of PRKCQ-AS1 and miR-582-3p in pathological tissues and LUAD cell lines. Additionally, a dual-luciferase reporter assay validated the reciprocal relationship. CCK8 examined cell proliferation, Transwell observed cell migration and invasion. Results PRKCQ-AS1 was down-regulated and miR-582-3p was up-regulated in LUAD tissues and cell. PRKCQ-AS1 and miR-582-3p expression affects some pathological features (lymph node metastasis, TNM stage, tumour differentiation) in LUAD patients. Patients with low PRKCQ-AS1 and high miR-582-3p had increased mortality. Interaction of PRKCQ-AS1 targeting miR-582-3p exists in LUAD cells. RGMB, STXBP6 are downstream target genes of miR-582-3p. Overexpression of (oe-) PRKCQ-AS1 inhibited LUAD cell proliferation, migration, and invasion. However, concomitant use of miR-582-3p mimics resisted the effects of PRKCQ-AS1 overexpression on cells. Conclusion PRKCQ-AS1/miR-582-3p axis regulatory relationship exists in lung adenocarcinoma cells. PRKCQ-AS1 may regulate the proliferation, migration and invasion of lung adenocarcinoma cells and participate in LUAD regulation by targeting miR-582-3p. Clinical trial number Not applicable.https://doi.org/10.1186/s41065-025-00482-9PRKCQ-AS1LUADmiR-582-3p |
| spellingShingle | Lingling Liu Xiaofen Liu Xiaojiao Wu Hang Fang Jingjing Shi Wei Jiang Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD Hereditas PRKCQ-AS1 LUAD miR-582-3p |
| title | Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD |
| title_full | Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD |
| title_fullStr | Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD |
| title_full_unstemmed | Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD |
| title_short | Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD |
| title_sort | clinical significance and biological function of prkcq as1 mir 582 3p expression in luad |
| topic | PRKCQ-AS1 LUAD miR-582-3p |
| url | https://doi.org/10.1186/s41065-025-00482-9 |
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