The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes
Enterococcus faecalis is a bacterial species present at a subdominant level in the human gut microbiota. This commensal turns into an opportunistic pathogen under specific conditions involving dysbiosis and host immune deficiency. E. faecalis is one of the rare pathobionts identified to date as cont...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2058851 |
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| author | Natalia Nunez Aurélie Derré-Bobillot Nicolas Trainel Goran Lakisic Alexandre Lecomte Françoise Mercier-Nomé Anne-Marie Cassard Hélène Bierne Pascale Serror Cristel Archambaud |
| author_facet | Natalia Nunez Aurélie Derré-Bobillot Nicolas Trainel Goran Lakisic Alexandre Lecomte Françoise Mercier-Nomé Anne-Marie Cassard Hélène Bierne Pascale Serror Cristel Archambaud |
| author_sort | Natalia Nunez |
| collection | DOAJ |
| description | Enterococcus faecalis is a bacterial species present at a subdominant level in the human gut microbiota. This commensal turns into an opportunistic pathogen under specific conditions involving dysbiosis and host immune deficiency. E. faecalis is one of the rare pathobionts identified to date as contributing to liver damage in alcoholic liver disease. We have previously observed that E. faecalis is internalized in hepatocytes. Here, the survival and fate of E. faecalis was examined in hepatocytes, the main epithelial cell type in the liver. Although referred to as an extracellular pathogen, we demonstrate that E. faecalis is able to survive and divide in hepatocytes, and form intracellular clusters in two distinct hepatocyte cell lines, in primary mouse hepatocytes, as well as in vivo. This novel process extends to kidney cells. Unraveling the intracellular lifestyle of E. faecalis, our findings contribute to the understanding of pathobiont-driven diseases. |
| format | Article |
| id | doaj-art-4da5d23142594d7dbc2b98bfe5f36104 |
| institution | OA Journals |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-4da5d23142594d7dbc2b98bfe5f361042025-08-20T02:29:59ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2058851The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytesNatalia Nunez0Aurélie Derré-Bobillot1Nicolas Trainel2Goran Lakisic3Alexandre Lecomte4Françoise Mercier-Nomé5Anne-Marie Cassard6Hélène Bierne7Pascale Serror8Cristel Archambaud9Université -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris-Saclay, Inserm U996, Inflammation, Microbiome and Immunosurveillance, Clamart, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris-Saclay, INSERM, CNRS, Institut Paris Saclay d’Innovation Thérapeutique, Châtenay-Malabry, FranceUniversité Paris-Saclay, Inserm U996, Inflammation, Microbiome and Immunosurveillance, Clamart, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité -Saclay, Inrae, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceEnterococcus faecalis is a bacterial species present at a subdominant level in the human gut microbiota. This commensal turns into an opportunistic pathogen under specific conditions involving dysbiosis and host immune deficiency. E. faecalis is one of the rare pathobionts identified to date as contributing to liver damage in alcoholic liver disease. We have previously observed that E. faecalis is internalized in hepatocytes. Here, the survival and fate of E. faecalis was examined in hepatocytes, the main epithelial cell type in the liver. Although referred to as an extracellular pathogen, we demonstrate that E. faecalis is able to survive and divide in hepatocytes, and form intracellular clusters in two distinct hepatocyte cell lines, in primary mouse hepatocytes, as well as in vivo. This novel process extends to kidney cells. Unraveling the intracellular lifestyle of E. faecalis, our findings contribute to the understanding of pathobiont-driven diseases.https://www.tandfonline.com/doi/10.1080/19490976.2022.2058851Enterococcus faecalispathobionthepatocytesintracellular lifestyleliver |
| spellingShingle | Natalia Nunez Aurélie Derré-Bobillot Nicolas Trainel Goran Lakisic Alexandre Lecomte Françoise Mercier-Nomé Anne-Marie Cassard Hélène Bierne Pascale Serror Cristel Archambaud The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes Gut Microbes Enterococcus faecalis pathobiont hepatocytes intracellular lifestyle liver |
| title | The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes |
| title_full | The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes |
| title_fullStr | The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes |
| title_full_unstemmed | The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes |
| title_short | The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes |
| title_sort | unforeseen intracellular lifestyle of enterococcus faecalis in hepatocytes |
| topic | Enterococcus faecalis pathobiont hepatocytes intracellular lifestyle liver |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2058851 |
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