Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2
Background: Early screening is critical for the prevention of ischemic stroke. miR-574–5p was considered a promising biomarker for ischemic stroke but lacks direct confirmation. This study evaluated miR-574–5p in discriminating ischemic stroke and predicting the severity and prognosis of patients, a...
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Elsevier
2025-02-01
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| Series: | Molecular and Cellular Probes |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S089085082500009X |
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| author | Xia Yin Chunlei Zhang |
| author_facet | Xia Yin Chunlei Zhang |
| author_sort | Xia Yin |
| collection | DOAJ |
| description | Background: Early screening is critical for the prevention of ischemic stroke. miR-574–5p was considered a promising biomarker for ischemic stroke but lacks direct confirmation. This study evaluated miR-574–5p in discriminating ischemic stroke and predicting the severity and prognosis of patients, aiming to provide novel insights into the clinical prevention of ischemic stroke. Methods: The clinical significance of miR-574–5p was evaluated in 103 ischemic stroke patients with 87 healthy individuals as control. The potential of serum miR-574–5p in the diagnosis and prognosis of ischemic stroke was assessed by ROC and logistic regression analyses. In vitro, oxygen-glucose deprivation (OGD)-induced microglia was established. The regulation of inflammation, oxidative stress, and proliferation of microglia by miR-574–5p were assessed by cell transfection. The downstream targets of miR-574–5p were predicted from public databases, and the targeting relationship was evaluated by luciferase reporter assay. Results: Reducing serum miR-574–5p was observed in ischemic stroke patients relative to healthy individuals, which discriminated ischemic stroke patients. Serum miR-574–5p was negatively correlated with the NIHSS score of ischemic stroke patients and was identified as a risk factor for patients’ adverse prognosis. In OGD-induced microglia, overexpressing miR-574–5p could alleviate OGD-induced inflammation and oxidative stress and promote cell growth. Among predicted targets, ATP2B2 was upregulated in ischemic stroke and showed a negative correlation with miR-574–5p. miR-574–5p negatively regulated ATP2B2 in OGD-induced microglia, and the overexpression of ATP2B2 reversed the protective effect of miR-574–5p. Conclusion: miR-574–5p acted as a biomarker for ischemic stroke and mediated neuroinflammation via targeting ATP2B2. |
| format | Article |
| id | doaj-art-4da3b417d6bb45bfa99067d0fa7ad5ed |
| institution | Kabale University |
| issn | 0890-8508 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Molecular and Cellular Probes |
| spelling | doaj-art-4da3b417d6bb45bfa99067d0fa7ad5ed2025-02-09T04:59:43ZengElsevierMolecular and Cellular Probes0890-85082025-02-0179102016Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2Xia Yin0Chunlei Zhang1Department of Rehabilitation Medicine, Weifang No.2 People's Hospital, Weifang, 261042, ChinaDepartment of Neonatology, Weifang Maternal and Child Health Hospital, Weifang, 261042, China; Corresponding author. Department of Neonatology, Weifang Maternal and Child Health Hospital, No.12007, Yingqian Street, Gaoxin District, Weifang, 261042, China.Background: Early screening is critical for the prevention of ischemic stroke. miR-574–5p was considered a promising biomarker for ischemic stroke but lacks direct confirmation. This study evaluated miR-574–5p in discriminating ischemic stroke and predicting the severity and prognosis of patients, aiming to provide novel insights into the clinical prevention of ischemic stroke. Methods: The clinical significance of miR-574–5p was evaluated in 103 ischemic stroke patients with 87 healthy individuals as control. The potential of serum miR-574–5p in the diagnosis and prognosis of ischemic stroke was assessed by ROC and logistic regression analyses. In vitro, oxygen-glucose deprivation (OGD)-induced microglia was established. The regulation of inflammation, oxidative stress, and proliferation of microglia by miR-574–5p were assessed by cell transfection. The downstream targets of miR-574–5p were predicted from public databases, and the targeting relationship was evaluated by luciferase reporter assay. Results: Reducing serum miR-574–5p was observed in ischemic stroke patients relative to healthy individuals, which discriminated ischemic stroke patients. Serum miR-574–5p was negatively correlated with the NIHSS score of ischemic stroke patients and was identified as a risk factor for patients’ adverse prognosis. In OGD-induced microglia, overexpressing miR-574–5p could alleviate OGD-induced inflammation and oxidative stress and promote cell growth. Among predicted targets, ATP2B2 was upregulated in ischemic stroke and showed a negative correlation with miR-574–5p. miR-574–5p negatively regulated ATP2B2 in OGD-induced microglia, and the overexpression of ATP2B2 reversed the protective effect of miR-574–5p. Conclusion: miR-574–5p acted as a biomarker for ischemic stroke and mediated neuroinflammation via targeting ATP2B2.http://www.sciencedirect.com/science/article/pii/S089085082500009XIschemic strokeHypertensionBiomarkerOnsetPrognosisMicroglia |
| spellingShingle | Xia Yin Chunlei Zhang Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 Molecular and Cellular Probes Ischemic stroke Hypertension Biomarker Onset Prognosis Microglia |
| title | Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 |
| title_full | Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 |
| title_fullStr | Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 |
| title_full_unstemmed | Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 |
| title_short | Circulating miR-574–5p shows diagnostic and prognostic significance and regulates oxygen-glucose deprivation (OGD)-induced inflammatory activation of microglia by targeting ATP2B2 |
| title_sort | circulating mir 574 5p shows diagnostic and prognostic significance and regulates oxygen glucose deprivation ogd induced inflammatory activation of microglia by targeting atp2b2 |
| topic | Ischemic stroke Hypertension Biomarker Onset Prognosis Microglia |
| url | http://www.sciencedirect.com/science/article/pii/S089085082500009X |
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