Comparison of absorption and excretion of test compounds in sucking versus chewing pests.
A critical understanding of how pests interact with active ingredients is essential for the development of new insect control solutions to maintain crop quality and quantity by reducing insect damage. Absorption of insecticides into insect bodies of targeted pest species is the first critical step t...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0321302 |
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| author | Clara I Römer Roman Ashauer Beate I Escher Juliane Hollender René Burkhard Kristin Höfer Michel Muehlebach Anke Buchholz |
| author_facet | Clara I Römer Roman Ashauer Beate I Escher Juliane Hollender René Burkhard Kristin Höfer Michel Muehlebach Anke Buchholz |
| author_sort | Clara I Römer |
| collection | DOAJ |
| description | A critical understanding of how pests interact with active ingredients is essential for the development of new insect control solutions to maintain crop quality and quantity by reducing insect damage. Absorption of insecticides into insect bodies of targeted pest species is the first critical step that confounds the efficacy of insecticides. This study investigated how different feeding behaviour of two pests, Myzus persicae and Spodoptera littoralis, affects the absorption, metabolism, and excretion (AME) of seven insecticidally inactive test compounds. A feeding contact assay for the chewing pest (Lepidopteran larvae) and an oral ingestion assay for the sucking pest (aphids) was used to investigate the AME of test compounds with agrochemical-like structural motifs. The standardized assays comprised of an exposure period with treated diet and a subsequent depuration period with untreated diet. The results showed that S. littoralis larvae differed from M. persicae in their compound quantities absorbed into the insect body and in their excretion products at the end of the exposure or depuration periods. We suggest that this is caused by their different ingestion types and rates resulting in different absorption and excretion quantities. Further, we found differences in the metabolism (timing and biotransformation pathways) of compounds between both species. Notably, certain compounds remained detectable in both pests after the depuration period, suggesting compound and species-specific metabolism and excretion. Our results highlight the complex interplay between feeding biology of insects, in particular the critical role of excretion products, and the exposure to different compounds that lead to species-specific AME. |
| format | Article |
| id | doaj-art-4da159980aba4ab39c1f649ac2c97ecc |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4da159980aba4ab39c1f649ac2c97ecc2025-08-20T03:52:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01204e032130210.1371/journal.pone.0321302Comparison of absorption and excretion of test compounds in sucking versus chewing pests.Clara I RömerRoman AshauerBeate I EscherJuliane HollenderRené BurkhardKristin HöferMichel MuehlebachAnke BuchholzA critical understanding of how pests interact with active ingredients is essential for the development of new insect control solutions to maintain crop quality and quantity by reducing insect damage. Absorption of insecticides into insect bodies of targeted pest species is the first critical step that confounds the efficacy of insecticides. This study investigated how different feeding behaviour of two pests, Myzus persicae and Spodoptera littoralis, affects the absorption, metabolism, and excretion (AME) of seven insecticidally inactive test compounds. A feeding contact assay for the chewing pest (Lepidopteran larvae) and an oral ingestion assay for the sucking pest (aphids) was used to investigate the AME of test compounds with agrochemical-like structural motifs. The standardized assays comprised of an exposure period with treated diet and a subsequent depuration period with untreated diet. The results showed that S. littoralis larvae differed from M. persicae in their compound quantities absorbed into the insect body and in their excretion products at the end of the exposure or depuration periods. We suggest that this is caused by their different ingestion types and rates resulting in different absorption and excretion quantities. Further, we found differences in the metabolism (timing and biotransformation pathways) of compounds between both species. Notably, certain compounds remained detectable in both pests after the depuration period, suggesting compound and species-specific metabolism and excretion. Our results highlight the complex interplay between feeding biology of insects, in particular the critical role of excretion products, and the exposure to different compounds that lead to species-specific AME.https://doi.org/10.1371/journal.pone.0321302 |
| spellingShingle | Clara I Römer Roman Ashauer Beate I Escher Juliane Hollender René Burkhard Kristin Höfer Michel Muehlebach Anke Buchholz Comparison of absorption and excretion of test compounds in sucking versus chewing pests. PLoS ONE |
| title | Comparison of absorption and excretion of test compounds in sucking versus chewing pests. |
| title_full | Comparison of absorption and excretion of test compounds in sucking versus chewing pests. |
| title_fullStr | Comparison of absorption and excretion of test compounds in sucking versus chewing pests. |
| title_full_unstemmed | Comparison of absorption and excretion of test compounds in sucking versus chewing pests. |
| title_short | Comparison of absorption and excretion of test compounds in sucking versus chewing pests. |
| title_sort | comparison of absorption and excretion of test compounds in sucking versus chewing pests |
| url | https://doi.org/10.1371/journal.pone.0321302 |
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