Fundamentals of protein interaction network mapping
Abstract Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the pa...
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| Format: | Article |
| Language: | English |
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Springer Nature
2015-12-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20156351 |
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| author | Jamie Snider Max Kotlyar Punit Saraon Zhong Yao Igor Jurisica Igor Stagljar |
| author_facet | Jamie Snider Max Kotlyar Punit Saraon Zhong Yao Igor Jurisica Igor Stagljar |
| author_sort | Jamie Snider |
| collection | DOAJ |
| description | Abstract Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the past two decades, diverse biochemical, genetic, and cell biological methods have been developed to map interactomes. In this review, we highlight basic principles of interactome mapping. Specifically, we discuss the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and provide general guidelines for carrying out the necessary follow‐up analyses. In addition, we discuss computational methods to predict, map, and visualize interactomes, and provide a summary of some of the most important interactome resources. We hope that this review serves as both a useful overview of the field and a guide to help more scientists actively employ these powerful approaches in their research. |
| format | Article |
| id | doaj-art-4d9c8b42698d4c37a5d54910239df75a |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-4d9c8b42698d4c37a5d54910239df75a2025-08-20T03:43:31ZengSpringer NatureMolecular Systems Biology1744-42922015-12-01111212010.15252/msb.20156351Fundamentals of protein interaction network mappingJamie Snider0Max Kotlyar1Punit Saraon2Zhong Yao3Igor Jurisica4Igor Stagljar5Donnelly Centre, Department of Biochemistry, Department of Molecular Genetics, University of TorontoPrincess Margaret Cancer Center, IBM Life Sciences Discovery Centre, University Health NetworkDonnelly Centre, Department of Biochemistry, Department of Molecular Genetics, University of TorontoDonnelly Centre, Department of Biochemistry, Department of Molecular Genetics, University of TorontoPrincess Margaret Cancer Center, IBM Life Sciences Discovery Centre, University Health NetworkDonnelly Centre, Department of Biochemistry, Department of Molecular Genetics, University of TorontoAbstract Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the past two decades, diverse biochemical, genetic, and cell biological methods have been developed to map interactomes. In this review, we highlight basic principles of interactome mapping. Specifically, we discuss the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and provide general guidelines for carrying out the necessary follow‐up analyses. In addition, we discuss computational methods to predict, map, and visualize interactomes, and provide a summary of some of the most important interactome resources. We hope that this review serves as both a useful overview of the field and a guide to help more scientists actively employ these powerful approaches in their research.https://doi.org/10.15252/msb.20156351bioinformaticsinteractome mappingPPI technologiesprotein‐protein interactions (PPIs)proteomics |
| spellingShingle | Jamie Snider Max Kotlyar Punit Saraon Zhong Yao Igor Jurisica Igor Stagljar Fundamentals of protein interaction network mapping Molecular Systems Biology bioinformatics interactome mapping PPI technologies protein‐protein interactions (PPIs) proteomics |
| title | Fundamentals of protein interaction network mapping |
| title_full | Fundamentals of protein interaction network mapping |
| title_fullStr | Fundamentals of protein interaction network mapping |
| title_full_unstemmed | Fundamentals of protein interaction network mapping |
| title_short | Fundamentals of protein interaction network mapping |
| title_sort | fundamentals of protein interaction network mapping |
| topic | bioinformatics interactome mapping PPI technologies protein‐protein interactions (PPIs) proteomics |
| url | https://doi.org/10.15252/msb.20156351 |
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