Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli
A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produ...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/895167 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106888738308096 |
|---|---|
| author | Neelima Deshpande Prachi Choubey Manasi Agashe |
| author_facet | Neelima Deshpande Prachi Choubey Manasi Agashe |
| author_sort | Neelima Deshpande |
| collection | DOAJ |
| description | A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production. |
| format | Article |
| id | doaj-art-4d9256c2ff6b47a583810c7d2d53bb9c |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4d9256c2ff6b47a583810c7d2d53bb9c2025-08-20T02:38:43ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/895167895167Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoliNeelima Deshpande0Prachi Choubey1Manasi Agashe2Department of Microbiology, Abasaheb Garware College, Pune 411004, IndiaDepartment of Microbiology, Abasaheb Garware College, Pune 411004, IndiaDepartment of Microbiology, Abasaheb Garware College, Pune 411004, IndiaA species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.http://dx.doi.org/10.1155/2014/895167 |
| spellingShingle | Neelima Deshpande Prachi Choubey Manasi Agashe Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli The Scientific World Journal |
| title | Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli |
| title_full | Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli |
| title_fullStr | Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli |
| title_full_unstemmed | Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli |
| title_short | Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli |
| title_sort | studies on optimization of growth parameters for l asparaginase production by streptomyces ginsengisoli |
| url | http://dx.doi.org/10.1155/2014/895167 |
| work_keys_str_mv | AT neelimadeshpande studiesonoptimizationofgrowthparametersforlasparaginaseproductionbystreptomycesginsengisoli AT prachichoubey studiesonoptimizationofgrowthparametersforlasparaginaseproductionbystreptomycesginsengisoli AT manasiagashe studiesonoptimizationofgrowthparametersforlasparaginaseproductionbystreptomycesginsengisoli |