Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia

Microorganisms play an important role in natural product discovery, particularly the member of genus Streptomyces. The Gram-positive, filamentous streptomycete, Streptomyces gilvigriseus MUSC 26T was firstly isolated from mangrove forest at Tanjung Lumpur, Malaysia. After 7-day fermentation, the sup...

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Main Authors: Hooi-Leng Ser, Wai-Fong Yin, Kok-Gan Chan, Tahir Mehmood Khan, Bey-Hing Goh, Learn-Han Lee
Format: Article
Language:English
Published: HH Publisher 2018-07-01
Series:Progress in Microbes and Molecular Biology
Online Access:https://journals.hh-publisher.com/index.php/pmmb/article/view/3
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author Hooi-Leng Ser
Wai-Fong Yin
Kok-Gan Chan
Tahir Mehmood Khan
Bey-Hing Goh
Learn-Han Lee
author_facet Hooi-Leng Ser
Wai-Fong Yin
Kok-Gan Chan
Tahir Mehmood Khan
Bey-Hing Goh
Learn-Han Lee
author_sort Hooi-Leng Ser
collection DOAJ
description Microorganisms play an important role in natural product discovery, particularly the member of genus Streptomyces. The Gram-positive, filamentous streptomycete, Streptomyces gilvigriseus MUSC 26T was firstly isolated from mangrove forest at Tanjung Lumpur, Malaysia. After 7-day fermentation, the supernatant of MUSC 26T was collected and subjected to chemical extraction. The resulting extract was used in antioxidant and invitro cytotoxicity assays against human colon cancer cell lines. The results showed that MUSC 26T extract possessed significant antioxidant activity and cytotoxic effect against the tested colon cancer cell lines. Lowest cell viability was observed in HCT-116 cell lines, at 63.64 ± 3.62 % (after treated with 400 μg/mL of extract). The other cell lines exhibited cell viability ranged from 67.50 – 86.66 % when treated with 400 μg/mL of extract. Further chemical analysis on the extraction using GC-MS has revealed the production of 14 compounds, including two cyclic dipeptides which could possibly contribute to the observed bioactivities. By the same token, the availability of its genome sequence allows further investigation into the biosynthetic gene clusters responsible for the production of these compounds which could essentially accelerate its development as chemopreventive drug(s).
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institution Kabale University
issn 2637-1049
language English
publishDate 2018-07-01
publisher HH Publisher
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series Progress in Microbes and Molecular Biology
spelling doaj-art-4d7e4fbe98a04d0fadfd208dedae85d72025-02-04T08:37:18ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492018-07-011110.36877/pmmb.a0000002Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in MalaysiaHooi-Leng SerWai-Fong YinKok-Gan ChanTahir Mehmood KhanBey-Hing GohLearn-Han LeeMicroorganisms play an important role in natural product discovery, particularly the member of genus Streptomyces. The Gram-positive, filamentous streptomycete, Streptomyces gilvigriseus MUSC 26T was firstly isolated from mangrove forest at Tanjung Lumpur, Malaysia. After 7-day fermentation, the supernatant of MUSC 26T was collected and subjected to chemical extraction. The resulting extract was used in antioxidant and invitro cytotoxicity assays against human colon cancer cell lines. The results showed that MUSC 26T extract possessed significant antioxidant activity and cytotoxic effect against the tested colon cancer cell lines. Lowest cell viability was observed in HCT-116 cell lines, at 63.64 ± 3.62 % (after treated with 400 μg/mL of extract). The other cell lines exhibited cell viability ranged from 67.50 – 86.66 % when treated with 400 μg/mL of extract. Further chemical analysis on the extraction using GC-MS has revealed the production of 14 compounds, including two cyclic dipeptides which could possibly contribute to the observed bioactivities. By the same token, the availability of its genome sequence allows further investigation into the biosynthetic gene clusters responsible for the production of these compounds which could essentially accelerate its development as chemopreventive drug(s).https://journals.hh-publisher.com/index.php/pmmb/article/view/3
spellingShingle Hooi-Leng Ser
Wai-Fong Yin
Kok-Gan Chan
Tahir Mehmood Khan
Bey-Hing Goh
Learn-Han Lee
Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
Progress in Microbes and Molecular Biology
title Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
title_full Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
title_fullStr Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
title_full_unstemmed Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
title_short Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia
title_sort antioxidant and cytotoxic potentials of streptomyces gilvigriseus musc 26t isolated from mangrove soil in malaysia
url https://journals.hh-publisher.com/index.php/pmmb/article/view/3
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