Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae
Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-no...
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| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1768805 |
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| author | Shijun Sun Hua Gao Yudong Liu Longyang Jin Ruobing Wang Xiaojuan Wang Qi Wang Yuyao Yin Yawei Zhang Hui Wang |
| author_facet | Shijun Sun Hua Gao Yudong Liu Longyang Jin Ruobing Wang Xiaojuan Wang Qi Wang Yuyao Yin Yawei Zhang Hui Wang |
| author_sort | Shijun Sun |
| collection | DOAJ |
| description | Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in our nationwide collection. Even worse, the tmexCD1-toprJ1 and mcr co-harbouring plasmid was also co-existed with blaNDM-harbouring IncX3 plasmid in the same host, resulting in pandrug resistance. Phylogenetic analysis suggested that the plasmid-borne tmexCD1-toprJ1 originated from the chromosome of Aeromonas spp. through Tn5393-mediating translocation. Both plasmid-harbored tmexCD1-toprJ1 gene and mcr-8 likely originated from animal isolates and then spread to human. Our findings highlight a substantial threat of tmexCD1-toprJ1-mcr8 co-harbouring IncFIA/IncFII plasmid to public health due to their mobile resistance to both tigecycline and colistin, emphasizing an urgent need for further global surveillance on this plasmid. |
| format | Article |
| id | doaj-art-4d79ef2d01cf4d7bb5beef5037f5a9bd |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-4d79ef2d01cf4d7bb5beef5037f5a9bd2025-08-20T02:12:15ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911102111310.1080/22221751.2020.1768805Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniaeShijun Sun0Hua Gao1Yudong Liu2Longyang Jin3Ruobing Wang4Xiaojuan Wang5Qi Wang6Yuyao Yin7Yawei Zhang8Hui Wang9Department of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of ChinaTigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in our nationwide collection. Even worse, the tmexCD1-toprJ1 and mcr co-harbouring plasmid was also co-existed with blaNDM-harbouring IncX3 plasmid in the same host, resulting in pandrug resistance. Phylogenetic analysis suggested that the plasmid-borne tmexCD1-toprJ1 originated from the chromosome of Aeromonas spp. through Tn5393-mediating translocation. Both plasmid-harbored tmexCD1-toprJ1 gene and mcr-8 likely originated from animal isolates and then spread to human. Our findings highlight a substantial threat of tmexCD1-toprJ1-mcr8 co-harbouring IncFIA/IncFII plasmid to public health due to their mobile resistance to both tigecycline and colistin, emphasizing an urgent need for further global surveillance on this plasmid.https://www.tandfonline.com/doi/10.1080/22221751.2020.1768805Plasmid-mediated tigecycline-resistant K. pneumoniaetmexCD1-toprJ1 efflux pumpmcr-8·5tmexCD1-mcr co-harbouring plasmidCRKP |
| spellingShingle | Shijun Sun Hua Gao Yudong Liu Longyang Jin Ruobing Wang Xiaojuan Wang Qi Wang Yuyao Yin Yawei Zhang Hui Wang Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae Emerging Microbes and Infections Plasmid-mediated tigecycline-resistant K. pneumoniae tmexCD1-toprJ1 efflux pump mcr-8·5 tmexCD1-mcr co-harbouring plasmid CRKP |
| title | Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae |
| title_full | Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae |
| title_fullStr | Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae |
| title_full_unstemmed | Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae |
| title_short | Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae |
| title_sort | co existence of a novel plasmid mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in klebsiella pneumoniae |
| topic | Plasmid-mediated tigecycline-resistant K. pneumoniae tmexCD1-toprJ1 efflux pump mcr-8·5 tmexCD1-mcr co-harbouring plasmid CRKP |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1768805 |
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