Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing

Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing

ObjectiveSingle-cell transcriptome sequencing is a powerful tool for investigating cellular diversity in normal development and disease. However, prevalent methods predominantly employ 3′-end sequencing of transcripts, limiting the analysis of alternative splicing and other post-transcriptional proc...

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Bibliographic Details
Main Authors: Tao Xu, Yicong Xu, Ziyang An, Yiheng Li, Jiawen Yang, Weixing Zhang, Jin Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Genetics
Subjects:
methanol fixation
RNA/DNA hybrids
full-length transcriptome
scRNA-seq
RNA junctions
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2025.1629655/full
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