Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis
Transforming growth factor (TGF-β1) is among the strongest factors of liver fibrogenesis, but its association with Schistosoma-caused liver fibrosis is controversial. Tissue transglutaminase (tTG) is the principal enzyme controlling TGF-β1 maturation and contributes to Sj-infected liver fibrosis. He...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/659378 |
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| author | Juanjuan Tang Xunmin Zhu Jingjing Zhao Mingchiu Fung Yinyan Li Zhiyan Gao Suikai Yan Xiaomin Li Xiaofang Ji Fang Su Zi Li |
| author_facet | Juanjuan Tang Xunmin Zhu Jingjing Zhao Mingchiu Fung Yinyan Li Zhiyan Gao Suikai Yan Xiaomin Li Xiaofang Ji Fang Su Zi Li |
| author_sort | Juanjuan Tang |
| collection | DOAJ |
| description | Transforming growth factor (TGF-β1) is among the strongest factors of liver fibrogenesis, but its association with Schistosoma-caused liver fibrosis is controversial. Tissue transglutaminase (tTG) is the principal enzyme controlling TGF-β1 maturation and contributes to Sj-infected liver fibrosis. Here we aim to explore the consistency between tTG and TGF-β1 and TGF-β1 source and its correlation with liver fibrosis after Sj-infection. TGF-β1 was upregulated at weeks 6 and 8 upon liver fibrosis induction. During tTG inhibition, TGF-β1 level decreased in sera and liver of infected mice. TGF-β1 showed positive staining in liver containing Sj adult worms and eggs. TGF-β1 was also detected in Sj adult worm sections, soluble egg antigen and Sj adult worm antigen, and adult worms’ culture medium. The TGF-β1 mature peptide cDNA sequence and its extended sequence were amplified through RT-PCR and RACE-PCR using adult worms as template, and sequence is analyzed and loaded to NCBI GenBank (number GQ338152.1). TGF-β1 transcript in Sj eggs was higher than in adult worms. In Sj-infected liver, transcriptional level of TGF-β1 from Sj, but not mouse liver, correlated with liver fibrosis extent. This study provides evidence that tTG regulates TGF-β1 and illustrates the importance of targeting tTG in treating Sj infection-induced fibrosis. |
| format | Article |
| id | doaj-art-4d687f04e19c41199193be33d73091d5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-4d687f04e19c41199193be33d73091d52025-08-20T03:36:21ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/659378659378Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver FibrosisJuanjuan Tang0Xunmin Zhu1Jingjing Zhao2Mingchiu Fung3Yinyan Li4Zhiyan Gao5Suikai Yan6Xiaomin Li7Xiaofang Ji8Fang Su9Zi Li10Guangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaMorphology Department, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaThe First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, ChinaSchool of Life Sciences, The Chinese University of Hong Kong, Hong KongMorphology Department, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaMorphology Department, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaMorphology Department, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaMorphology Department, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaGuangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaGuangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaGuangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, ChinaTransforming growth factor (TGF-β1) is among the strongest factors of liver fibrogenesis, but its association with Schistosoma-caused liver fibrosis is controversial. Tissue transglutaminase (tTG) is the principal enzyme controlling TGF-β1 maturation and contributes to Sj-infected liver fibrosis. Here we aim to explore the consistency between tTG and TGF-β1 and TGF-β1 source and its correlation with liver fibrosis after Sj-infection. TGF-β1 was upregulated at weeks 6 and 8 upon liver fibrosis induction. During tTG inhibition, TGF-β1 level decreased in sera and liver of infected mice. TGF-β1 showed positive staining in liver containing Sj adult worms and eggs. TGF-β1 was also detected in Sj adult worm sections, soluble egg antigen and Sj adult worm antigen, and adult worms’ culture medium. The TGF-β1 mature peptide cDNA sequence and its extended sequence were amplified through RT-PCR and RACE-PCR using adult worms as template, and sequence is analyzed and loaded to NCBI GenBank (number GQ338152.1). TGF-β1 transcript in Sj eggs was higher than in adult worms. In Sj-infected liver, transcriptional level of TGF-β1 from Sj, but not mouse liver, correlated with liver fibrosis extent. This study provides evidence that tTG regulates TGF-β1 and illustrates the importance of targeting tTG in treating Sj infection-induced fibrosis.http://dx.doi.org/10.1155/2015/659378 |
| spellingShingle | Juanjuan Tang Xunmin Zhu Jingjing Zhao Mingchiu Fung Yinyan Li Zhiyan Gao Suikai Yan Xiaomin Li Xiaofang Ji Fang Su Zi Li Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis Mediators of Inflammation |
| title | Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis |
| title_full | Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis |
| title_fullStr | Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis |
| title_full_unstemmed | Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis |
| title_short | Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis |
| title_sort | tissue transglutaminase regulated transformed growth factor β1 in the parasite links schistosoma japonicum infection with liver fibrosis |
| url | http://dx.doi.org/10.1155/2015/659378 |
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