Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients
Introduction: Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for p...
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The Journal of Infection in Developing Countries
2020-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12966 |
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| author | Juan Manuel Bello-López Carlos Sanchez- Garibay Gabriela Ibáñez-Cervantes Gregorio León-García Luis Uriel Gonzalez-Avila Cecilia Hernández-Cortez Patricia Arzate Barbosa Graciela Castro-Escarpulli |
| author_facet | Juan Manuel Bello-López Carlos Sanchez- Garibay Gabriela Ibáñez-Cervantes Gregorio León-García Luis Uriel Gonzalez-Avila Cecilia Hernández-Cortez Patricia Arzate Barbosa Graciela Castro-Escarpulli |
| author_sort | Juan Manuel Bello-López |
| collection | DOAJ |
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Introduction: Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim of this work was to detect the pheno/genotype of β-lactamases and class 1 integrons in Aeromonas spp., strains isolated from pediatric patients in a tertiary referral hospital in Mexico.
Methodology: Sixty-six strains of Aeromonas spp., were isolated from clinical samples of pediatric origin and were identified by RFLP-PCR 16S rRNA. Resistance phenotype according to CLSI, genetic and phenotypic detection of extended-spectrum β-lactamases (ESBL) and metallo-b-lactamases (MBL) was performed. Finally, characterization of class 1 integrons was performed.
Results: Aeromonas spp., strains of diarrheic origin were more predominant. A wide heterogeneity was detected, where A. caviae was the predominant specie. Second-generation cephalosporins, fluoroquinolones, and nitrofurans had best antimicrobial activity; moreover, antibiotics of the β-lactamic and lincosamides families showed lower inhibitory activity. Phenotypically, prevalences of 4.55% and 3.03% were detected for MBL (intestinal origin) and ESBL (extraintestinal origin), respectively. blaIMIS-cphA and blaTEM-1 genes, and nineteen class 1 integrons carrying two variants of cassettes corresponding to adenylyl transferases (aadA), and dihydrofolate reductases (dfrA). Monogenic array with aadA1 cassette was predominantly.
Conclusions: ESBL and class 1 integrons, in Aeromonas collected from pediatric patients, determines a major detection challenge for the clinical microbiology laboratory and represents a remarkable epidemiological risk of nosocomial spread of multidrug-resistant determinants.
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| format | Article |
| id | doaj-art-4d555e3b6b594d8ba08490d8f47fbd8a |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-4d555e3b6b594d8ba08490d8f47fbd8a2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-10-01141010.3855/jidc.12966Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patientsJuan Manuel Bello-López0Carlos Sanchez- Garibay1Gabriela Ibáñez-Cervantes2Gregorio León-García3Luis Uriel Gonzalez-Avila4Cecilia Hernández-Cortez5Patricia Arzate Barbosa6Graciela Castro-Escarpulli7División de Investigación, Hospital Juárez de México, Ciudad de México, MéxicoDepartamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MéxicoDivisión de Investigación, Hospital Juárez de México, Ciudad de México, MéxicoHospital de la Mujer, Ciudad de México, MéxicoLaboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MéxicoLaboratorio Bioquímica Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MéxicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Autónoma de México, Ciudad de México, MéxicoLaboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México Introduction: Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim of this work was to detect the pheno/genotype of β-lactamases and class 1 integrons in Aeromonas spp., strains isolated from pediatric patients in a tertiary referral hospital in Mexico. Methodology: Sixty-six strains of Aeromonas spp., were isolated from clinical samples of pediatric origin and were identified by RFLP-PCR 16S rRNA. Resistance phenotype according to CLSI, genetic and phenotypic detection of extended-spectrum β-lactamases (ESBL) and metallo-b-lactamases (MBL) was performed. Finally, characterization of class 1 integrons was performed. Results: Aeromonas spp., strains of diarrheic origin were more predominant. A wide heterogeneity was detected, where A. caviae was the predominant specie. Second-generation cephalosporins, fluoroquinolones, and nitrofurans had best antimicrobial activity; moreover, antibiotics of the β-lactamic and lincosamides families showed lower inhibitory activity. Phenotypically, prevalences of 4.55% and 3.03% were detected for MBL (intestinal origin) and ESBL (extraintestinal origin), respectively. blaIMIS-cphA and blaTEM-1 genes, and nineteen class 1 integrons carrying two variants of cassettes corresponding to adenylyl transferases (aadA), and dihydrofolate reductases (dfrA). Monogenic array with aadA1 cassette was predominantly. Conclusions: ESBL and class 1 integrons, in Aeromonas collected from pediatric patients, determines a major detection challenge for the clinical microbiology laboratory and represents a remarkable epidemiological risk of nosocomial spread of multidrug-resistant determinants. https://jidc.org/index.php/journal/article/view/12966Aeromonas spp.antimicrobial resistanceclass 1 integronsβ-Lactamases |
| spellingShingle | Juan Manuel Bello-López Carlos Sanchez- Garibay Gabriela Ibáñez-Cervantes Gregorio León-García Luis Uriel Gonzalez-Avila Cecilia Hernández-Cortez Patricia Arzate Barbosa Graciela Castro-Escarpulli Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients Journal of Infection in Developing Countries Aeromonas spp. antimicrobial resistance class 1 integrons β-Lactamases |
| title | Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients |
| title_full | Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients |
| title_fullStr | Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients |
| title_full_unstemmed | Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients |
| title_short | Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients |
| title_sort | genetic and phenotypic determinants of resistance to antibiotics in aeromonas spp strains isolated from pediatric patients |
| topic | Aeromonas spp. antimicrobial resistance class 1 integrons β-Lactamases |
| url | https://jidc.org/index.php/journal/article/view/12966 |
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