Expression of tumor necrosis factor alpha and kidney injury molecule-1 in the kidney and serum of rats subjected to acute renal ischemic reperfusion injury
Objective To observe the expression of tumor necrosis factor alpha(TNF-α),and kidney injury molecule-1(Kim-1)in the kidney tissues and serum of rats subjected to acute renal ischemic reperfusion injury.Methods Ninety-six SD male rats were randomly divided into Sham group(n=48)and ischemic/reperfusio...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2016-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57919473&Fpath=home&index=0 |
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| Summary: | Objective To observe the expression of tumor necrosis factor alpha(TNF-α),and kidney injury molecule-1(Kim-1)in the kidney tissues and serum of rats subjected to acute renal ischemic reperfusion injury.Methods Ninety-six SD male rats were randomly divided into Sham group(n=48)and ischemic/reperfusion group(I/R,n=48).The bilateral arteries were blocked to make the IR models.Eight rats from the two groups were randomly selected 2,6,12,24,48 and 72 h after the surgery,and the blood and renal cortex collected.Serum nitrogen and creatinine were evaluated by biochemical autoanalyzer.The concentrations of TNF-α and Kim-1 were measured by ELISA.The TNF-α and Kim-1 expression in kidney tissues was detected by immunohistochemistry.Results As compared with Sham group,the BUN and SCr began to increase in I/R group at 6 h after I-R(for BUN,50.8±6.8 vs.79.6±8.8 μmol/L,and for SCr,(4.7±0.5)vs.(9.3±1.6)mmol/L,P<0.05 for both).ELISA revealed that the serum TNF-α and Kim-1 were increased at 2 h after I/R in I/R group as compared with Sham group(for TNF-α,944.2±68.3 vs.843.0±60.5;for Kim-1,1081.3±126.2 vs.453.7±56.4,P<0.05 for both),which reached a peak at 48 h after reperfusion,but TNF-α was decreased obviously after 72 h(3 094.4±230.5),and Kim-1 was kept the highest level from 48 to 72 h.Immunohistochemistry showed that TNF-a and Kim-1 were mainly expressed on the tubular area and increased significantly at 12 h after I/R Conclusions Serum TNF-α and Kim-1 might be appropriate biological markers of acute kidney injury,and Kim-1 might be better. |
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| ISSN: | 1671-2390 |