Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing
Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical propert...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112019 |
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| _version_ | 1850153868315328512 |
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| author | Mya Breitbart Forest Rohwer |
| author_facet | Mya Breitbart Forest Rohwer |
| author_sort | Mya Breitbart |
| collection | DOAJ |
| description | Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both single- and double-stranded DNA viruses can be recovered from blood samples using this approach. In addition, we report the discovery of novel anellovirus sequences in the blood of healthy donors. PCR primers designed to amplify these novel anellovirus sequences were then used to verify the presence of these viruses in the general donor population. |
| format | Article |
| id | doaj-art-4d421b1f8e65401b9195aba931e76ba7 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2005-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-4d421b1f8e65401b9195aba931e76ba72025-08-20T02:25:37ZengTaylor & Francis GroupBioTechniques0736-62051940-98182005-11-0139572973610.2144/000112019Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencingMya Breitbart0Forest Rohwer11San Diego State University, San Diego, CA, USA1San Diego State University, San Diego, CA, USARapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both single- and double-stranded DNA viruses can be recovered from blood samples using this approach. In addition, we report the discovery of novel anellovirus sequences in the blood of healthy donors. PCR primers designed to amplify these novel anellovirus sequences were then used to verify the presence of these viruses in the general donor population.https://www.future-science.com/doi/10.2144/000112019 |
| spellingShingle | Mya Breitbart Forest Rohwer Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing BioTechniques |
| title | Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing |
| title_full | Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing |
| title_fullStr | Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing |
| title_full_unstemmed | Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing |
| title_short | Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing |
| title_sort | method for discovering novel dna viruses in blood using viral particle selection and shotgun sequencing |
| url | https://www.future-science.com/doi/10.2144/000112019 |
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