In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates
The degradation of Tetra-PEG hydrogels containing β-eliminative crosslinks has been studied in order to provide an in vitro-in vivo correlation for the use of these hydrogels in our chemically controlled drug delivery system. We measured time-dependent gel mass loss and ultrasound volume changes of...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2019/9483127 |
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| author | Jeff Henise Shaun D. Fontaine Brian R. Hearn Samuel J. Pfaff Eric L. Schneider Julia Malato Donghui Wang Byron Hann Gary W. Ashley Daniel V. Santi |
| author_facet | Jeff Henise Shaun D. Fontaine Brian R. Hearn Samuel J. Pfaff Eric L. Schneider Julia Malato Donghui Wang Byron Hann Gary W. Ashley Daniel V. Santi |
| author_sort | Jeff Henise |
| collection | DOAJ |
| description | The degradation of Tetra-PEG hydrogels containing β-eliminative crosslinks has been studied in order to provide an in vitro-in vivo correlation for the use of these hydrogels in our chemically controlled drug delivery system. We measured time-dependent gel mass loss and ultrasound volume changes of 13 subcutaneously implanted Tetra-PEG hydrogel microspheres having degradation times ranging from ~3 to 250 days. Applying a previously developed model of Tetra-PEG hydrogel degradation, the mass changes correlate well with the in vitro rates of crosslink cleavage and hydrogel degelation. These results allow prediction of in vivo biodegradation properties of these hydrogels based on readily obtained in vitro rates, despite having degradation times that span 2 orders of magnitude. These results support the optimization of drug-releasing hydrogels and their development into long-acting therapeutics. The use of ultrasound volume measurements further provides a noninvasive technique for monitoring hydrogel degradation in the subcutaneous space. |
| format | Article |
| id | doaj-art-4d40cbe04a32493b86028345287c8f5e |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-4d40cbe04a32493b86028345287c8f5e2025-08-20T03:36:21ZengWileyInternational Journal of Polymer Science1687-94221687-94302019-01-01201910.1155/2019/94831279483127In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage RatesJeff Henise0Shaun D. Fontaine1Brian R. Hearn2Samuel J. Pfaff3Eric L. Schneider4Julia Malato5Donghui Wang6Byron Hann7Gary W. Ashley8Daniel V. Santi9ProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAHelen Diller Family Comprehensive Cancer Center University of California, San Francisco, 1450 3rd St., San Francisco, CA 94158, USAHelen Diller Family Comprehensive Cancer Center University of California, San Francisco, 1450 3rd St., San Francisco, CA 94158, USAHelen Diller Family Comprehensive Cancer Center University of California, San Francisco, 1450 3rd St., San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAProLynx, 455 Mission Bay Blvd. South, San Francisco, CA 94158, USAThe degradation of Tetra-PEG hydrogels containing β-eliminative crosslinks has been studied in order to provide an in vitro-in vivo correlation for the use of these hydrogels in our chemically controlled drug delivery system. We measured time-dependent gel mass loss and ultrasound volume changes of 13 subcutaneously implanted Tetra-PEG hydrogel microspheres having degradation times ranging from ~3 to 250 days. Applying a previously developed model of Tetra-PEG hydrogel degradation, the mass changes correlate well with the in vitro rates of crosslink cleavage and hydrogel degelation. These results allow prediction of in vivo biodegradation properties of these hydrogels based on readily obtained in vitro rates, despite having degradation times that span 2 orders of magnitude. These results support the optimization of drug-releasing hydrogels and their development into long-acting therapeutics. The use of ultrasound volume measurements further provides a noninvasive technique for monitoring hydrogel degradation in the subcutaneous space.http://dx.doi.org/10.1155/2019/9483127 |
| spellingShingle | Jeff Henise Shaun D. Fontaine Brian R. Hearn Samuel J. Pfaff Eric L. Schneider Julia Malato Donghui Wang Byron Hann Gary W. Ashley Daniel V. Santi In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates International Journal of Polymer Science |
| title | In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates |
| title_full | In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates |
| title_fullStr | In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates |
| title_full_unstemmed | In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates |
| title_short | In Vitro-In Vivo Correlation for the Degradation of Tetra-PEG Hydrogel Microspheres with Tunable β-Eliminative Crosslink Cleavage Rates |
| title_sort | in vitro in vivo correlation for the degradation of tetra peg hydrogel microspheres with tunable β eliminative crosslink cleavage rates |
| url | http://dx.doi.org/10.1155/2019/9483127 |
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