Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease
Background:. Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and...
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| Format: | Article |
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Wolters Kluwer
2025-05-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006806 |
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| author | Anastasios Topalis, MD Chrysanthi Bekiari, PhD Efterpi Demiri, MD, PhD Dimitrios Dionyssiou, MD, PhD |
| author_facet | Anastasios Topalis, MD Chrysanthi Bekiari, PhD Efterpi Demiri, MD, PhD Dimitrios Dionyssiou, MD, PhD |
| author_sort | Anastasios Topalis, MD |
| collection | DOAJ |
| description | Background:. Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and affect neurodegeneration by transferring amyloid-beta peptides from the brain to the cervical lymph nodes. We aimed to assess the feasibility of a novel operation in a rat model, to enhance the meningeal lymphatic system and consequently increase the clearance of amyloid-beta peptide.
Methods:. Ten 12-month-old rats were divided into 2 groups. Group A (control) had no intervention. Group B underwent collagen scaffold implantation between the subdural space above the hippocampus and the right submandibular lymph node, to achieve guided lymphangiogenesis. Animals were weighed and examined for neurological, behavioral and cognitive tests preoperatively and 6 and 12 weeks postoperatively, including NeuroScale, modified open field, and novel object location recognition tests.
Results:. One animal of group B died 2 days postoperatively. The remaining rats had no complications, maintained or increased their weight, and showed no signs of infection or neurological impairment. Behavioral tests were in favor of group B at 12 weeks postoperative. Animals of group A had statistically significant decline in cognitive function over time. Animals of group B demonstrated the same or better cognitive function.
Conclusions:. In this pilot study, we demonstrated the feasibility of the proposed surgical procedure, which resulted in positive cognitive function for the animals. We are now utilizing an AD rat model to evaluate the possible therapeutic effect of the procedure. |
| format | Article |
| id | doaj-art-4d3caa1f1ed14a2ab643f75733e793da |
| institution | DOAJ |
| issn | 2169-7574 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj-art-4d3caa1f1ed14a2ab643f75733e793da2025-08-20T03:14:27ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742025-05-01135e680610.1097/GOX.0000000000006806202505000-00071Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer DiseaseAnastasios Topalis, MD0Chrysanthi Bekiari, PhD1Efterpi Demiri, MD, PhD2Dimitrios Dionyssiou, MD, PhD3From the * Department of Plastic Surgery, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece† Laboratory of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University, Thessaloniki, Greece.From the * Department of Plastic Surgery, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceFrom the * Department of Plastic Surgery, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceBackground:. Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and affect neurodegeneration by transferring amyloid-beta peptides from the brain to the cervical lymph nodes. We aimed to assess the feasibility of a novel operation in a rat model, to enhance the meningeal lymphatic system and consequently increase the clearance of amyloid-beta peptide. Methods:. Ten 12-month-old rats were divided into 2 groups. Group A (control) had no intervention. Group B underwent collagen scaffold implantation between the subdural space above the hippocampus and the right submandibular lymph node, to achieve guided lymphangiogenesis. Animals were weighed and examined for neurological, behavioral and cognitive tests preoperatively and 6 and 12 weeks postoperatively, including NeuroScale, modified open field, and novel object location recognition tests. Results:. One animal of group B died 2 days postoperatively. The remaining rats had no complications, maintained or increased their weight, and showed no signs of infection or neurological impairment. Behavioral tests were in favor of group B at 12 weeks postoperative. Animals of group A had statistically significant decline in cognitive function over time. Animals of group B demonstrated the same or better cognitive function. Conclusions:. In this pilot study, we demonstrated the feasibility of the proposed surgical procedure, which resulted in positive cognitive function for the animals. We are now utilizing an AD rat model to evaluate the possible therapeutic effect of the procedure.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006806 |
| spellingShingle | Anastasios Topalis, MD Chrysanthi Bekiari, PhD Efterpi Demiri, MD, PhD Dimitrios Dionyssiou, MD, PhD Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease Plastic and Reconstructive Surgery, Global Open |
| title | Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease |
| title_full | Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease |
| title_fullStr | Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease |
| title_full_unstemmed | Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease |
| title_short | Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease |
| title_sort | pilot study lymphatic brain decongestion a new surgical technique in alzheimer disease |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006806 |
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