Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells
Background: Hematopoietic stem cell expansion relies on direct cell-cell interactions mediated by adhesion molecules, integrins, and cytokines. Unrestricted somatic stem cells have emerged as novel stromal cells supporting hematopoietic stem cell expansion in co-culture conditions via secretion of h...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Hematology, Transfusion and Cell Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137925000951 |
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| author | Sanaz Khaseb Mahdi Kohansal Vajari Mina Soufi Zomorrod Maryam Rezai Rad Monireh Ajami Mansoureh Ajami Saba Sadeghpour Amir Atashi |
| author_facet | Sanaz Khaseb Mahdi Kohansal Vajari Mina Soufi Zomorrod Maryam Rezai Rad Monireh Ajami Mansoureh Ajami Saba Sadeghpour Amir Atashi |
| author_sort | Sanaz Khaseb |
| collection | DOAJ |
| description | Background: Hematopoietic stem cell expansion relies on direct cell-cell interactions mediated by adhesion molecules, integrins, and cytokines. Unrestricted somatic stem cells have emerged as novel stromal cells supporting hematopoietic stem cell expansion in co-culture conditions via secretion of hematopoiesis-related cytokines and the expression of adhesion molecules. Previous research showed fibrin increased hematopoiesis-related gene expression in these cells. This study focused on the adhesive characteristics of unrestricted somatic stem cells on 3D fibrin scaffolds. Methods: Unrestricted somatic stem cells were isolated from umbilical cord blood and characterized using flow cytometry and multilineage differentiation assays. Scanning electron microscopy and DAPI staining were employed to analyze cell attachment to fibrin. Viability on fibrin was assessed through MTT assays. Quantitative polymerase chain reaction was conducted to evaluate the expression of intercellular adhesion molecule 1 (ICAM-1), integrin subunit αv (ITGAV), and integrin subunit β3 (ITGB3) in cells cultured on 3D fibrin scaffolds. Results: Cells were positive for CD73, CD105, and CD166 but negative for CD45. Alizarin red and Oil red O stains confirmed calcium deposition and lipid vacuoles. MTT assays revealed that fibrin positively impacts viability. ITGAV expression was significantly increased in cells cultured on fibrin compared to those cultured on plastic tissue culture plates (Control Group). Furthermore, ITGB3 expression showed no significant change in both groups, while ICAM-1 expression was downregulated in cells cultured on fibrin. Conclusions: Our study revealed that fibrin has a positive impact on the expression of ITGAV, which plays a crucial role in direct cell-cell interactions affecting hematopoietic stem cell expansion. |
| format | Article |
| id | doaj-art-4d3bde7a64bf4601a97d41727f9d2dbe |
| institution | DOAJ |
| issn | 2531-1379 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj-art-4d3bde7a64bf4601a97d41727f9d2dbe2025-08-20T03:08:28ZengElsevierHematology, Transfusion and Cell Therapy2531-13792025-04-0147210382710.1016/j.htct.2025.103827Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cellsSanaz Khaseb0Mahdi Kohansal Vajari1Mina Soufi Zomorrod2Maryam Rezai Rad3Monireh Ajami4Mansoureh Ajami5Saba Sadeghpour6Amir Atashi7Faculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, IranFaculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran; School of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, IranFaculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, IranResearch Institute for Dental Sciences, Dental Research Center, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, IranFaculty of Paramedical Sciences, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, IranSchool of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, IranSchool of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranStem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran; Corresponding author.Background: Hematopoietic stem cell expansion relies on direct cell-cell interactions mediated by adhesion molecules, integrins, and cytokines. Unrestricted somatic stem cells have emerged as novel stromal cells supporting hematopoietic stem cell expansion in co-culture conditions via secretion of hematopoiesis-related cytokines and the expression of adhesion molecules. Previous research showed fibrin increased hematopoiesis-related gene expression in these cells. This study focused on the adhesive characteristics of unrestricted somatic stem cells on 3D fibrin scaffolds. Methods: Unrestricted somatic stem cells were isolated from umbilical cord blood and characterized using flow cytometry and multilineage differentiation assays. Scanning electron microscopy and DAPI staining were employed to analyze cell attachment to fibrin. Viability on fibrin was assessed through MTT assays. Quantitative polymerase chain reaction was conducted to evaluate the expression of intercellular adhesion molecule 1 (ICAM-1), integrin subunit αv (ITGAV), and integrin subunit β3 (ITGB3) in cells cultured on 3D fibrin scaffolds. Results: Cells were positive for CD73, CD105, and CD166 but negative for CD45. Alizarin red and Oil red O stains confirmed calcium deposition and lipid vacuoles. MTT assays revealed that fibrin positively impacts viability. ITGAV expression was significantly increased in cells cultured on fibrin compared to those cultured on plastic tissue culture plates (Control Group). Furthermore, ITGB3 expression showed no significant change in both groups, while ICAM-1 expression was downregulated in cells cultured on fibrin. Conclusions: Our study revealed that fibrin has a positive impact on the expression of ITGAV, which plays a crucial role in direct cell-cell interactions affecting hematopoietic stem cell expansion.http://www.sciencedirect.com/science/article/pii/S2531137925000951Adhesion moleculeExpansionFibrinUmbilical cord bloodUnrestricted somatic stromal cells |
| spellingShingle | Sanaz Khaseb Mahdi Kohansal Vajari Mina Soufi Zomorrod Maryam Rezai Rad Monireh Ajami Mansoureh Ajami Saba Sadeghpour Amir Atashi Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells Hematology, Transfusion and Cell Therapy Adhesion molecule Expansion Fibrin Umbilical cord blood Unrestricted somatic stromal cells |
| title | Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells |
| title_full | Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells |
| title_fullStr | Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells |
| title_full_unstemmed | Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells |
| title_short | Effect of fibrin on the expression of adhesion molecules (ICAM-1, ITGAV, and ITGB3) in unrestricted somatic stem cells |
| title_sort | effect of fibrin on the expression of adhesion molecules icam 1 itgav and itgb3 in unrestricted somatic stem cells |
| topic | Adhesion molecule Expansion Fibrin Umbilical cord blood Unrestricted somatic stromal cells |
| url | http://www.sciencedirect.com/science/article/pii/S2531137925000951 |
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