Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide
Mastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic b...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2023.2290527 |
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| author | Jingjing Liu Yingkui Gao Xing Zhang Zhonghua Hao Huaqiang Zhang Rong Gui Fang Liu Chao Tong Xuebing Wang |
| author_facet | Jingjing Liu Yingkui Gao Xing Zhang Zhonghua Hao Huaqiang Zhang Rong Gui Fang Liu Chao Tong Xuebing Wang |
| author_sort | Jingjing Liu |
| collection | DOAJ |
| description | Mastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by transcriptome sequencing. The results showed that compared to the control group, the LPS treatment group had 121 up-regulated genes and 18 down-regulated genes. GO and KEGG enrichment analysis revealed that these differential genes were mainly enriched in the IL-17 signaling pathway, Legionellosis, Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, and other signaling pathways. Furthermore, the expression of GRO1 and CXCL3 mRNAs increased significantly after LPS treatment. These findings provide new insights for the treatment of mastitis in cows in the future. |
| format | Article |
| id | doaj-art-4d3a944d68db4755afa4a13ad51ea136 |
| institution | DOAJ |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-4d3a944d68db4755afa4a13ad51ea1362025-08-20T03:13:50ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2023.2290527Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharideJingjing Liu0Yingkui Gao1Xing Zhang2Zhonghua Hao3Huaqiang Zhang4Rong Gui5Fang Liu6Chao Tong7Xuebing Wang8College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, P. R. ChinaMastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by transcriptome sequencing. The results showed that compared to the control group, the LPS treatment group had 121 up-regulated genes and 18 down-regulated genes. GO and KEGG enrichment analysis revealed that these differential genes were mainly enriched in the IL-17 signaling pathway, Legionellosis, Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, and other signaling pathways. Furthermore, the expression of GRO1 and CXCL3 mRNAs increased significantly after LPS treatment. These findings provide new insights for the treatment of mastitis in cows in the future.https://www.tandfonline.com/doi/10.1080/10495398.2023.2290527Cow mastitisLPStranscriptional sequencing technologyMAC-T |
| spellingShingle | Jingjing Liu Yingkui Gao Xing Zhang Zhonghua Hao Huaqiang Zhang Rong Gui Fang Liu Chao Tong Xuebing Wang Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide Animal Biotechnology Cow mastitis LPS transcriptional sequencing technology MAC-T |
| title | Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| title_full | Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| title_fullStr | Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| title_full_unstemmed | Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| title_short | Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| title_sort | transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide |
| topic | Cow mastitis LPS transcriptional sequencing technology MAC-T |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2023.2290527 |
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