Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis
Chronic kidney disease (CKD) can cause gut microbiota dysbiosis and decreasing production of short-chain fatty acids (SCFAs), which aggravate the injury of kidney. It has been found that a variety of Chinese medicine polysaccharides can regulate gut microbiota, especially probiotics, and have benefi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2429693 |
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| author | Wenbo Liu Yuanyuan Zhang Dongmei Hu Lihua Huang Xusheng Liu Zhaoyu Lu |
| author_facet | Wenbo Liu Yuanyuan Zhang Dongmei Hu Lihua Huang Xusheng Liu Zhaoyu Lu |
| author_sort | Wenbo Liu |
| collection | DOAJ |
| description | Chronic kidney disease (CKD) can cause gut microbiota dysbiosis and decreasing production of short-chain fatty acids (SCFAs), which aggravate the injury of kidney. It has been found that a variety of Chinese medicine polysaccharides can regulate gut microbiota, especially probiotics, and have beneficial effects on human health. Astragalus polysaccharide (APS) is a major component of Astragalus aceus. The aim of this study was to investigate whether APS can regulate gut microbiota–SCFAs to slow the progression of CKD. Adenine-induced CKD mice (Ade) were established and APS was treated. The renal protection of APS on CKD mice was evaluated by renal function and pathological staining of renal tissues. Feces samples were collected for 16SrRNA sequence and LC–MS/MS analysis. Kidney G protein-coupled receptor (GPR) levels were also detected in renal tissue. APS supplementation can reduce serum creatinine and urea nitrogen levels in mice model (Ade) and attenuate renal tubular interstitial injury and renal fibrosis. Further application of 16SrRNA sequencing showed that the abundance of SCFA producing bacteria, such as Kineothrix, Faecalibaculum, Akkermansia, Lactobacillus, and Roseburia, was upregulated after APS treatment. Fecal LC–MS/MS detection showed that the levels of acetate, propionate and butyrate in Ade mice increased after APS supplementation. The detection of renal GPRs showed that APS supplementing could significantly increase the levels of renal GPR41 and GPR43, and also partially increase the levels of GPR109a in Ade mice. Our research confirms that APS supplementation can upregulate the abundance of SCFA producing bacteria and increase SCFA levels to attenuate renal tubular interstitial injury and fibrosis via GPRs. |
| format | Article |
| id | doaj-art-4d33eb3bb4f840a09f0e4e423331f35f |
| institution | OA Journals |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-4d33eb3bb4f840a09f0e4e423331f35f2025-08-20T02:38:11ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2429693Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axisWenbo Liu0Yuanyuan Zhang1Dongmei Hu2Lihua Huang3Xusheng Liu4Zhaoyu Lu5State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaChronic kidney disease (CKD) can cause gut microbiota dysbiosis and decreasing production of short-chain fatty acids (SCFAs), which aggravate the injury of kidney. It has been found that a variety of Chinese medicine polysaccharides can regulate gut microbiota, especially probiotics, and have beneficial effects on human health. Astragalus polysaccharide (APS) is a major component of Astragalus aceus. The aim of this study was to investigate whether APS can regulate gut microbiota–SCFAs to slow the progression of CKD. Adenine-induced CKD mice (Ade) were established and APS was treated. The renal protection of APS on CKD mice was evaluated by renal function and pathological staining of renal tissues. Feces samples were collected for 16SrRNA sequence and LC–MS/MS analysis. Kidney G protein-coupled receptor (GPR) levels were also detected in renal tissue. APS supplementation can reduce serum creatinine and urea nitrogen levels in mice model (Ade) and attenuate renal tubular interstitial injury and renal fibrosis. Further application of 16SrRNA sequencing showed that the abundance of SCFA producing bacteria, such as Kineothrix, Faecalibaculum, Akkermansia, Lactobacillus, and Roseburia, was upregulated after APS treatment. Fecal LC–MS/MS detection showed that the levels of acetate, propionate and butyrate in Ade mice increased after APS supplementation. The detection of renal GPRs showed that APS supplementing could significantly increase the levels of renal GPR41 and GPR43, and also partially increase the levels of GPR109a in Ade mice. Our research confirms that APS supplementation can upregulate the abundance of SCFA producing bacteria and increase SCFA levels to attenuate renal tubular interstitial injury and fibrosis via GPRs.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2429693Chronic kidney diseasegut microbiotaAstragalus polysaccharideshort-chain fatty acidsrenal protective |
| spellingShingle | Wenbo Liu Yuanyuan Zhang Dongmei Hu Lihua Huang Xusheng Liu Zhaoyu Lu Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis Renal Failure Chronic kidney disease gut microbiota Astragalus polysaccharide short-chain fatty acids renal protective |
| title | Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis |
| title_full | Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis |
| title_fullStr | Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis |
| title_full_unstemmed | Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis |
| title_short | Oral Astragalus polysaccharide alleviates adenine-induced kidney injury by regulating gut microbiota–short-chain fatty acids–kidney G protein-coupled receptors axis |
| title_sort | oral astragalus polysaccharide alleviates adenine induced kidney injury by regulating gut microbiota short chain fatty acids kidney g protein coupled receptors axis |
| topic | Chronic kidney disease gut microbiota Astragalus polysaccharide short-chain fatty acids renal protective |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2429693 |
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