Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success
Cytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammato...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/606459 |
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| author | Aili Sarapik Agne Velthut Kadri Haller-Kikkatalo Gilbert C. Faure Marie-Christine Béné Marcelo de Carvalho Bittencourt Frédéric Massin Raivo Uibo Andres Salumets |
| author_facet | Aili Sarapik Agne Velthut Kadri Haller-Kikkatalo Gilbert C. Faure Marie-Christine Béné Marcelo de Carvalho Bittencourt Frédéric Massin Raivo Uibo Andres Salumets |
| author_sort | Aili Sarapik |
| collection | DOAJ |
| description | Cytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammatory: G-CSF), chemokines (MIP-1α, MIP-1β, MCP-1, RANTES, and IL-8), and other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoing in vitro fertilization (IVF). Cytokine origin was studied by mRNA analysis of granulosa cells. Higher follicular MIP-1α and CD44(v6) were found to correlate with polycystic ovary syndrome, IL-23, INF-γ, and TNF-α with endometriosis, higher CD44(v6) but lower IL-β and INF-α correlated with tubal factor infertility, and lower levels of IL-18 and CD44(v6) characterized unexplained infertility. IL-12 positively correlated with oocyte fertilization and embryo development, while increased IL-18, IL-8, and MIP-1β were associated with successful IVF-induced pregnancy. |
| format | Article |
| id | doaj-art-4d3017158dde4246a154776f87718e0d |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-4d3017158dde4246a154776f87718e0d2025-08-20T02:38:38ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/606459606459Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF SuccessAili Sarapik0Agne Velthut1Kadri Haller-Kikkatalo2Gilbert C. Faure3Marie-Christine Béné4Marcelo de Carvalho Bittencourt5Frédéric Massin6Raivo Uibo7Andres Salumets8Department of Immunology, Institute of General and Molecular Pathology, University of Tartu, Ravila 19, Tartu 50411, EstoniaInstitute of Gene Technology, Tallinn University of Technology, Akadeemia Street 15, Tallinn 12618, EstoniaDepartment of Immunology, Institute of General and Molecular Pathology, University of Tartu, Ravila 19, Tartu 50411, EstoniaLaboratoire d'Immunologie, Université Henri Poincaré, BP 184, Vandoeuvre lès Nancy, 54500 Nancy, FranceLaboratoire d'Immunologie, Université Henri Poincaré, BP 184, Vandoeuvre lès Nancy, 54500 Nancy, FranceLaboratoire d'Immunologie, Université Henri Poincaré, BP 184, Vandoeuvre lès Nancy, 54500 Nancy, FranceLaboratoire d'Immunologie, Université Henri Poincaré, BP 184, Vandoeuvre lès Nancy, 54500 Nancy, FranceDepartment of Immunology, Institute of General and Molecular Pathology, University of Tartu, Ravila 19, Tartu 50411, EstoniaCompetence Centre on Reproductive Medicine and Biology, Tiigi 61b, Tartu 50410, EstoniaCytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammatory: G-CSF), chemokines (MIP-1α, MIP-1β, MCP-1, RANTES, and IL-8), and other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoing in vitro fertilization (IVF). Cytokine origin was studied by mRNA analysis of granulosa cells. Higher follicular MIP-1α and CD44(v6) were found to correlate with polycystic ovary syndrome, IL-23, INF-γ, and TNF-α with endometriosis, higher CD44(v6) but lower IL-β and INF-α correlated with tubal factor infertility, and lower levels of IL-18 and CD44(v6) characterized unexplained infertility. IL-12 positively correlated with oocyte fertilization and embryo development, while increased IL-18, IL-8, and MIP-1β were associated with successful IVF-induced pregnancy.http://dx.doi.org/10.1155/2012/606459 |
| spellingShingle | Aili Sarapik Agne Velthut Kadri Haller-Kikkatalo Gilbert C. Faure Marie-Christine Béné Marcelo de Carvalho Bittencourt Frédéric Massin Raivo Uibo Andres Salumets Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success Clinical and Developmental Immunology |
| title | Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success |
| title_full | Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success |
| title_fullStr | Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success |
| title_full_unstemmed | Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success |
| title_short | Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success |
| title_sort | follicular proinflammatory cytokines and chemokines as markers of ivf success |
| url | http://dx.doi.org/10.1155/2012/606459 |
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