Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme
Abstract The emergence of multidrug-resistant Klebsiella pneumoniae poses significant clinical challenges with limited treatment options. Biofilm is an important virulence factor of K. pneumoniae, serving as a protective barrier against antibiotics and the immune system. Here, we present the remarka...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-024-00593-7 |
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| author | Reshma Ramakrishnan Abhilash V. Nair Kirti Parmar Raju S. Rajmani Dipshikha Chakravortty Debasis Das |
| author_facet | Reshma Ramakrishnan Abhilash V. Nair Kirti Parmar Raju S. Rajmani Dipshikha Chakravortty Debasis Das |
| author_sort | Reshma Ramakrishnan |
| collection | DOAJ |
| description | Abstract The emergence of multidrug-resistant Klebsiella pneumoniae poses significant clinical challenges with limited treatment options. Biofilm is an important virulence factor of K. pneumoniae, serving as a protective barrier against antibiotics and the immune system. Here, we present the remarkable ability of a bovine microbial enzyme to prevent biofilm formation (IC50 2.50 μM) and degrade pre-formed K. pneumoniae biofilms (EC50 1.94 μM) by degrading the matrix polysaccharides. The treatment was effective against four different clinical K. pneumoniae isolates tested. Moreover, the enzyme significantly improved the biofilm sensitivity of a poorly performing broad-spectrum antibiotic, meropenem, and immune cells, resulting in facile biofilm clearance from the mouse wound infection. Notably, well-known powerful enzymes of the same class, cellulase, and α-amylase, were nearly inactive against the K. pneumoniae biofilms. The enzyme exhibited antibiofilm activity without showing toxicity to the mammalian and microbial cells, highlighting the potential of the enzyme for in vivo applications. |
| format | Article |
| id | doaj-art-4d2b57c4ff874ac1970ab40feeca6c46 |
| institution | DOAJ |
| issn | 2055-5008 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-4d2b57c4ff874ac1970ab40feeca6c462025-08-20T02:50:00ZengNature Portfolionpj Biofilms and Microbiomes2055-50082024-11-0110111810.1038/s41522-024-00593-7Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzymeReshma Ramakrishnan0Abhilash V. Nair1Kirti Parmar2Raju S. Rajmani3Dipshikha Chakravortty4Debasis Das5Department of Inorganic and Physical Chemistry, Indian Institute of ScienceDepartment of Microbiology and Cell Biology, Indian Institute of ScienceDepartment of Microbiology and Cell Biology, Indian Institute of ScienceMolecular Biophysics Unit, Indian Institute of ScienceDepartment of Microbiology and Cell Biology, Indian Institute of ScienceDepartment of Inorganic and Physical Chemistry, Indian Institute of ScienceAbstract The emergence of multidrug-resistant Klebsiella pneumoniae poses significant clinical challenges with limited treatment options. Biofilm is an important virulence factor of K. pneumoniae, serving as a protective barrier against antibiotics and the immune system. Here, we present the remarkable ability of a bovine microbial enzyme to prevent biofilm formation (IC50 2.50 μM) and degrade pre-formed K. pneumoniae biofilms (EC50 1.94 μM) by degrading the matrix polysaccharides. The treatment was effective against four different clinical K. pneumoniae isolates tested. Moreover, the enzyme significantly improved the biofilm sensitivity of a poorly performing broad-spectrum antibiotic, meropenem, and immune cells, resulting in facile biofilm clearance from the mouse wound infection. Notably, well-known powerful enzymes of the same class, cellulase, and α-amylase, were nearly inactive against the K. pneumoniae biofilms. The enzyme exhibited antibiofilm activity without showing toxicity to the mammalian and microbial cells, highlighting the potential of the enzyme for in vivo applications.https://doi.org/10.1038/s41522-024-00593-7 |
| spellingShingle | Reshma Ramakrishnan Abhilash V. Nair Kirti Parmar Raju S. Rajmani Dipshikha Chakravortty Debasis Das Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme npj Biofilms and Microbiomes |
| title | Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme |
| title_full | Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme |
| title_fullStr | Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme |
| title_full_unstemmed | Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme |
| title_short | Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme |
| title_sort | combating biofilm associated klebsiella pneumoniae infections using a bovine microbial enzyme |
| url | https://doi.org/10.1038/s41522-024-00593-7 |
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