Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration
Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fis...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2024-12-01
|
| Series: | Smart Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/SMMD.20240056 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850119971295723520 |
|---|---|
| author | Haozhen Ren Danqing Huang Mengdi Qiu Lingling Xue Shaoshi Zhu Jingjing Gan Cheng Chen Dayu Chen Jinglin Wang |
| author_facet | Haozhen Ren Danqing Huang Mengdi Qiu Lingling Xue Shaoshi Zhu Jingjing Gan Cheng Chen Dayu Chen Jinglin Wang |
| author_sort | Haozhen Ren |
| collection | DOAJ |
| description | Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish liver extracellular matrix for liver regeneration. By decellularizing fish liver and combining it with gelatin methacryloyl, the hydrogel scaffold retains essential endogenous growth factors such as collagen and glycosaminoglycans. Additionally, microfluidic‐assisted 3D printing technology enables precise modulation of the composition and architecture of hydrogels to fulfill clinical requirements. Benefiting from the natural source of materials, the hydrogels exhibit excellent biocompatibility and cellular proliferation capacity for incorporating induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, the macroscopic architecture and biomechanical environment of hydrogels foster optimal functional expression of iPSC‐heps. Importantly, post‐transplantation, the hydrogels significantly enhance survival rates and liver function in mice with acute liver failure, promoting liver regeneration and repair. These findings suggest that microfluidic 3D printed hydrogels represent promising candidates for liver transplantation and functional recovery. |
| format | Article |
| id | doaj-art-4d2440c18ace44dca9bbe8442d6ea49c |
| institution | OA Journals |
| issn | 2751-1871 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Smart Medicine |
| spelling | doaj-art-4d2440c18ace44dca9bbe8442d6ea49c2025-08-20T02:35:30ZengWiley-VCHSmart Medicine2751-18712024-12-0134n/an/a10.1002/SMMD.20240056Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regenerationHaozhen Ren0Danqing Huang1Mengdi Qiu2Lingling Xue3Shaoshi Zhu4Jingjing Gan5Cheng Chen6Dayu Chen7Jinglin Wang8Division of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaDivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaDivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaDivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaCollege of Medicine University of Illinois Chicago Illinois USADivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaDivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaSchool of Pharmacy Faculty of Medicine Macau University of Science and Technology Macau ChinaDivision of Hepatobiliary and Transplantation Surgery Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing ChinaAbstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish liver extracellular matrix for liver regeneration. By decellularizing fish liver and combining it with gelatin methacryloyl, the hydrogel scaffold retains essential endogenous growth factors such as collagen and glycosaminoglycans. Additionally, microfluidic‐assisted 3D printing technology enables precise modulation of the composition and architecture of hydrogels to fulfill clinical requirements. Benefiting from the natural source of materials, the hydrogels exhibit excellent biocompatibility and cellular proliferation capacity for incorporating induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, the macroscopic architecture and biomechanical environment of hydrogels foster optimal functional expression of iPSC‐heps. Importantly, post‐transplantation, the hydrogels significantly enhance survival rates and liver function in mice with acute liver failure, promoting liver regeneration and repair. These findings suggest that microfluidic 3D printed hydrogels represent promising candidates for liver transplantation and functional recovery.https://doi.org/10.1002/SMMD.202400563D printingdecellularized extracellular matrixhydrogelliver regenerationmicrofluidic |
| spellingShingle | Haozhen Ren Danqing Huang Mengdi Qiu Lingling Xue Shaoshi Zhu Jingjing Gan Cheng Chen Dayu Chen Jinglin Wang Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration Smart Medicine 3D printing decellularized extracellular matrix hydrogel liver regeneration microfluidic |
| title | Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| title_full | Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| title_fullStr | Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| title_full_unstemmed | Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| title_short | Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| title_sort | microfluidic 3d printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration |
| topic | 3D printing decellularized extracellular matrix hydrogel liver regeneration microfluidic |
| url | https://doi.org/10.1002/SMMD.20240056 |
| work_keys_str_mv | AT haozhenren microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT danqinghuang microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT mengdiqiu microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT linglingxue microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT shaoshizhu microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT jingjinggan microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT chengchen microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT dayuchen microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration AT jinglinwang microfluidic3dprintinghydrogelsbasedonfishliverdecellularizedextracellularmatrixforliverregeneration |