Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce

To explore the metabolite composition and bacterial phase composition of Hubei traditional wheat sauce, the physicochemical indexes determination, non-targeted metabolomics analysis and dominant strains isolation were conducted in this study. The results showed that both wheat sauces met the require...

Full description

Saved in:
Bibliographic Details
Main Authors: Qing LI, Qihe CHEN, Xiayu LIU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-06-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070257
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850260625690722304
author Qing LI
Qihe CHEN
Xiayu LIU
author_facet Qing LI
Qihe CHEN
Xiayu LIU
author_sort Qing LI
collection DOAJ
description To explore the metabolite composition and bacterial phase composition of Hubei traditional wheat sauce, the physicochemical indexes determination, non-targeted metabolomics analysis and dominant strains isolation were conducted in this study. The results showed that both wheat sauces met the requirements of GB 2718-2014 National Food Safety Standard for Brewing Sauce. A total of 236 non-volatile metabolites were identified by liquid chromatograph-tandem mass spectrometry (LC-MS/MS), mainly organic acids and their derivatives, fatty acyls, benzene and substituted derivatives, among which 195 non-volatile differential metabolites were screened. Moreover, 178 volatile metabolites were identified by gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS), mainly organic acids and their derivatives, organic oxygen compounds, lipids and lipid-like molecules, among which 106 volatile differential metabolites were screened. The results of bacterial species identification indicated that the dominant species were Bacillus, Aspergillus oryzae and Starmerella etchellsii. Collectively, this study laid a solid theoretical foundation for the analysis of flavor composition and identification of dominant bacteria in traditional wheat sauce.
format Article
id doaj-art-4d1e7593d2f84ff78f0a331b95e040ac
institution OA Journals
issn 1002-0306
language zho
publishDate 2025-06-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj-art-4d1e7593d2f84ff78f0a331b95e040ac2025-08-20T01:55:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-014611324310.13386/j.issn1002-0306.20240702572024070257-11Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat SauceQing LI0Qihe CHEN1Xiayu LIU2College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310000, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310000, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310000, ChinaTo explore the metabolite composition and bacterial phase composition of Hubei traditional wheat sauce, the physicochemical indexes determination, non-targeted metabolomics analysis and dominant strains isolation were conducted in this study. The results showed that both wheat sauces met the requirements of GB 2718-2014 National Food Safety Standard for Brewing Sauce. A total of 236 non-volatile metabolites were identified by liquid chromatograph-tandem mass spectrometry (LC-MS/MS), mainly organic acids and their derivatives, fatty acyls, benzene and substituted derivatives, among which 195 non-volatile differential metabolites were screened. Moreover, 178 volatile metabolites were identified by gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS), mainly organic acids and their derivatives, organic oxygen compounds, lipids and lipid-like molecules, among which 106 volatile differential metabolites were screened. The results of bacterial species identification indicated that the dominant species were Bacillus, Aspergillus oryzae and Starmerella etchellsii. Collectively, this study laid a solid theoretical foundation for the analysis of flavor composition and identification of dominant bacteria in traditional wheat sauce.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070257fermented flour saucewheat saucephysical and chemical indicatorsidentification of flavor substancesdominant species
spellingShingle Qing LI
Qihe CHEN
Xiayu LIU
Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
Shipin gongye ke-ji
fermented flour sauce
wheat sauce
physical and chemical indicators
identification of flavor substances
dominant species
title Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
title_full Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
title_fullStr Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
title_full_unstemmed Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
title_short Analysis of Metabolite Composition and Isolation of Dominant Bacteria in Two Hubei Special Wheat Sauce
title_sort analysis of metabolite composition and isolation of dominant bacteria in two hubei special wheat sauce
topic fermented flour sauce
wheat sauce
physical and chemical indicators
identification of flavor substances
dominant species
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070257
work_keys_str_mv AT qingli analysisofmetabolitecompositionandisolationofdominantbacteriaintwohubeispecialwheatsauce
AT qihechen analysisofmetabolitecompositionandisolationofdominantbacteriaintwohubeispecialwheatsauce
AT xiayuliu analysisofmetabolitecompositionandisolationofdominantbacteriaintwohubeispecialwheatsauce