Bioethanol production from edible insect excreta: a case study on frass from house crickets
House crickets are among the most promising edible insect species for inclusion in future agri-food systems. Their appeal stems from environmentally sustainable rearing practices, a high nutritional value, and a long history of traditional use as food. Additionally, their rearing produces a byproduc...
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Alpha Creation Enterprise
2024-12-01
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| Series: | Biofuel Research Journal |
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| Online Access: | https://www.biofueljournal.com/article_210402_d766d2dd701fe5f73967d23fd420201f.pdf |
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| author | Marios Psarianos Roland Schneider Önder Altuntas George Dimopoulos Petros Taoukis Oliver Schlüter |
| author_facet | Marios Psarianos Roland Schneider Önder Altuntas George Dimopoulos Petros Taoukis Oliver Schlüter |
| author_sort | Marios Psarianos |
| collection | DOAJ |
| description | House crickets are among the most promising edible insect species for inclusion in future agri-food systems. Their appeal stems from environmentally sustainable rearing practices, a high nutritional value, and a long history of traditional use as food. Additionally, their rearing produces a byproduct known as frass, which holds potential as a valuable biomaterial. The utilization of house cricket frass as a substrate for bioethanol production was explored. Frass was digested with cellulases at 10% enzyme/dry matter of substrate, 50°C, pH=5, 48 h. This hydrolysis was combined with different treatments, like acidic (1% sulfuric acid) and alkaline (1% sodium hydroxide), and compared to protease treatment (50°C, pH=6.8, 24 h). The production of sugar and free amino proteins reached 30 and 5 g/L, respectively. Several yeast strains, isolated and identified from various organic waste sources, were tested. The fermentation was performed with Saccharomyces cerevisiae for 48 h with frass hydrolysate, pretreated with sulfuric acid, and digested with proteases and cellulases. The addition of molasses at 0‒60 g/L was considered. Sugar consumption exceeded 80%, with ethanol concentrations reaching 12.56 g/L without molasses and 30.57 g/L with the addition of molasses. Cricket frass was utilized as a substrate for bioethanol production, and the process was significantly enhanced by supplementing it with sugar beet molasses. |
| format | Article |
| id | doaj-art-4d0af491368f43b685492f8d9b29ea96 |
| institution | OA Journals |
| issn | 2292-8782 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Alpha Creation Enterprise |
| record_format | Article |
| series | Biofuel Research Journal |
| spelling | doaj-art-4d0af491368f43b685492f8d9b29ea962025-08-20T02:07:12ZengAlpha Creation EnterpriseBiofuel Research Journal2292-87822024-12-011142211222410.18331/BRJ2024.11.4.2210402Bioethanol production from edible insect excreta: a case study on frass from house cricketsMarios Psarianos0Roland Schneider1Önder Altuntas2George Dimopoulos3Petros Taoukis4Oliver Schlüter5System Process Engineering, Leibniz institute for agricultural engineering and bioeconomy, Max-Eyth-Allee 100, 14469 Potsdam, Germany.Microbiome Biotechnology, Leibniz institute for agricultural engineering and bioeconomy, Max-Eyth-Allee 100, 14469 Potsdam, Germany.Faculty of Aeronautics and Astronautics, Eskisehir Technical University, 26470, Eskisehir, Türkiye.Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens (NTUA), Iroon Polytechniou 5, 15780 Athens, Greece.Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens (NTUA), Iroon Polytechniou 5, 15780 Athens, Greece.System Process Engineering, Leibniz institute for agricultural engineering and bioeconomy, Max-Eyth-Allee 100, 14469 Potsdam, Germany.House crickets are among the most promising edible insect species for inclusion in future agri-food systems. Their appeal stems from environmentally sustainable rearing practices, a high nutritional value, and a long history of traditional use as food. Additionally, their rearing produces a byproduct known as frass, which holds potential as a valuable biomaterial. The utilization of house cricket frass as a substrate for bioethanol production was explored. Frass was digested with cellulases at 10% enzyme/dry matter of substrate, 50°C, pH=5, 48 h. This hydrolysis was combined with different treatments, like acidic (1% sulfuric acid) and alkaline (1% sodium hydroxide), and compared to protease treatment (50°C, pH=6.8, 24 h). The production of sugar and free amino proteins reached 30 and 5 g/L, respectively. Several yeast strains, isolated and identified from various organic waste sources, were tested. The fermentation was performed with Saccharomyces cerevisiae for 48 h with frass hydrolysate, pretreated with sulfuric acid, and digested with proteases and cellulases. The addition of molasses at 0‒60 g/L was considered. Sugar consumption exceeded 80%, with ethanol concentrations reaching 12.56 g/L without molasses and 30.57 g/L with the addition of molasses. Cricket frass was utilized as a substrate for bioethanol production, and the process was significantly enhanced by supplementing it with sugar beet molasses.https://www.biofueljournal.com/article_210402_d766d2dd701fe5f73967d23fd420201f.pdfedible insectsfrasshydrolysisfermentationbioethanolbiofuel |
| spellingShingle | Marios Psarianos Roland Schneider Önder Altuntas George Dimopoulos Petros Taoukis Oliver Schlüter Bioethanol production from edible insect excreta: a case study on frass from house crickets Biofuel Research Journal edible insects frass hydrolysis fermentation bioethanol biofuel |
| title | Bioethanol production from edible insect excreta: a case study on frass from house crickets |
| title_full | Bioethanol production from edible insect excreta: a case study on frass from house crickets |
| title_fullStr | Bioethanol production from edible insect excreta: a case study on frass from house crickets |
| title_full_unstemmed | Bioethanol production from edible insect excreta: a case study on frass from house crickets |
| title_short | Bioethanol production from edible insect excreta: a case study on frass from house crickets |
| title_sort | bioethanol production from edible insect excreta a case study on frass from house crickets |
| topic | edible insects frass hydrolysis fermentation bioethanol biofuel |
| url | https://www.biofueljournal.com/article_210402_d766d2dd701fe5f73967d23fd420201f.pdf |
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