Comparative study of conventional Papanicolaou and ultrafast Papanicolaou stains in fine needle aspiration samples and body fluids

Background: Ultra Papanicolaou stain was introduced as a hybrid of Romanowsky and Papanicolaou stains. It enhances the quality and reduces the time. Materials and Methods: Five hundred and ninety-five fine needle aspiration and body fluid samples were collected. The fine needle aspiration procedur...

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Bibliographic Details
Main Authors: Prabesh Choudhary, Niraj Nepal, Rashmita Bhandari, Ritu Baral, Oshan Shrestha, Sarita Choudhary
Format: Article
Language:English
Published: Association of Clinical Pathologists of Nepal 2024-12-01
Series:Journal of Pathology of Nepal
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Online Access:https://www.nepjol.info/index.php/JPN/article/view/71320
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Summary:Background: Ultra Papanicolaou stain was introduced as a hybrid of Romanowsky and Papanicolaou stains. It enhances the quality and reduces the time. Materials and Methods: Five hundred and ninety-five fine needle aspiration and body fluid samples were collected. The fine needle aspiration procedure was performed by standard method; smears were fixed in 95% propanol and stained with conventional and ultra Papanicolaou stains. Four parameters were considered and scored (background, cell morphology, nuclear staining, and overall staining pattern). Results: The quality of ultra Papanicolaou stain smears was better than conventional pap stain, and this difference was statistically significant. Conclusions: UltraPap stain, compared to conventional Pap stain, provides an excellent alternative in cytological staining for the evaluation of various organs and fluids for benign and malignant pathology.
ISSN:2091-0797
2091-0908