Secreted alkaline ribonucleases of micromycetes
Many ribonucleases (RNases) have a selective cytotoxic effect on tumor cells. Of particular interest are enzymes that are phylogenetically distant from mammalian RNases, such as amphibian, fungal, and bacterial RNases, all insensitive to the action of the mammalian RNAse inhibitor. We screened 15 mi...
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Kazan Federal University
2020-09-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2020-3-1.html |
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| author | V.V. Ulyanova P.Yu. Vankov P.V. Zelenikhin R. Shah Mahmud A.I. Kolpakov O.N. Ilinskaya |
| author_facet | V.V. Ulyanova P.Yu. Vankov P.V. Zelenikhin R. Shah Mahmud A.I. Kolpakov O.N. Ilinskaya |
| author_sort | V.V. Ulyanova |
| collection | DOAJ |
| description | Many ribonucleases (RNases) have a selective cytotoxic effect on tumor cells. Of particular interest are enzymes that are phylogenetically distant from mammalian RNases, such as amphibian, fungal, and bacterial RNases, all insensitive to the action of the mammalian RNAse inhibitor. We screened 15 micromycete isolates for the presence of secreted alkaline ribonuclease. Seven isolates demonstrated the highest ribonucleolytic activity. Based on the phylogenetic analysis, the most active of them were identified as Fusarium sporotrichioides. The maximum level of activity (19000 units/mL) in the culture fluid of F. sporotrichioides was recorded on the third day of growth of the fungus. The obtained data on the thermal stability of the secreted F. sporotrichioides RNase, its molecular weight (11 kDa), and biosynthesis on a medium with a low phosphate content suggest that the enzyme belongs to the RNase T1/N1/U2 family. The culture fluid of F. sporotrichioides grown on the phosphate-deficient medium reduced the viability of A549 human lung adenocarcinoma cells by 16.3%; if the micromycete was grown on a medium with a high phosphate content, no toxicity of the culture fluid was detected. Thus, the secreted RNAse contributes to cytotoxicity. Further description and comprehensive study of the properties of the new RNase will help to establish the mechanisms of its cytotoxicity and identify the areas of application of the enzyme. |
| format | Article |
| id | doaj-art-4cebb417f3db4e0e9b9a5e78e0a70c7b |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-4cebb417f3db4e0e9b9a5e78e0a70c7b2025-01-02T21:15:00ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2020-09-01162333534910.26907/2542-064X.2020.3.335-349Secreted alkaline ribonucleases of micromycetesV.V. Ulyanova0P.Yu. Vankov1P.V. Zelenikhin2R. Shah Mahmud3A.I. Kolpakov4O.N. Ilinskaya5Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaMany ribonucleases (RNases) have a selective cytotoxic effect on tumor cells. Of particular interest are enzymes that are phylogenetically distant from mammalian RNases, such as amphibian, fungal, and bacterial RNases, all insensitive to the action of the mammalian RNAse inhibitor. We screened 15 micromycete isolates for the presence of secreted alkaline ribonuclease. Seven isolates demonstrated the highest ribonucleolytic activity. Based on the phylogenetic analysis, the most active of them were identified as Fusarium sporotrichioides. The maximum level of activity (19000 units/mL) in the culture fluid of F. sporotrichioides was recorded on the third day of growth of the fungus. The obtained data on the thermal stability of the secreted F. sporotrichioides RNase, its molecular weight (11 kDa), and biosynthesis on a medium with a low phosphate content suggest that the enzyme belongs to the RNase T1/N1/U2 family. The culture fluid of F. sporotrichioides grown on the phosphate-deficient medium reduced the viability of A549 human lung adenocarcinoma cells by 16.3%; if the micromycete was grown on a medium with a high phosphate content, no toxicity of the culture fluid was detected. Thus, the secreted RNAse contributes to cytotoxicity. Further description and comprehensive study of the properties of the new RNase will help to establish the mechanisms of its cytotoxicity and identify the areas of application of the enzyme.https://kpfu.ru/uz-eng-ns-2020-3-1.htmlmicromycetesfusariumribonucleasethermostabilitycytotoxicitya549 lung adenocarcinoma cells |
| spellingShingle | V.V. Ulyanova P.Yu. Vankov P.V. Zelenikhin R. Shah Mahmud A.I. Kolpakov O.N. Ilinskaya Secreted alkaline ribonucleases of micromycetes Учёные записки Казанского университета: Серия Естественные науки micromycetes fusarium ribonuclease thermostability cytotoxicity a549 lung adenocarcinoma cells |
| title | Secreted alkaline ribonucleases of micromycetes |
| title_full | Secreted alkaline ribonucleases of micromycetes |
| title_fullStr | Secreted alkaline ribonucleases of micromycetes |
| title_full_unstemmed | Secreted alkaline ribonucleases of micromycetes |
| title_short | Secreted alkaline ribonucleases of micromycetes |
| title_sort | secreted alkaline ribonucleases of micromycetes |
| topic | micromycetes fusarium ribonuclease thermostability cytotoxicity a549 lung adenocarcinoma cells |
| url | https://kpfu.ru/uz-eng-ns-2020-3-1.html |
| work_keys_str_mv | AT vvulyanova secretedalkalineribonucleasesofmicromycetes AT pyuvankov secretedalkalineribonucleasesofmicromycetes AT pvzelenikhin secretedalkalineribonucleasesofmicromycetes AT rshahmahmud secretedalkalineribonucleasesofmicromycetes AT aikolpakov secretedalkalineribonucleasesofmicromycetes AT onilinskaya secretedalkalineribonucleasesofmicromycetes |