The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae
Magnaporthe oryzae is the causal agent of rice blast. Glycosylation plays key roles in vegetative growth, development, and infection of M. oryzae. However, several glycosylation-related genes have not been characterized. In this study, we identified a Glyco_transf_22 domain-containing protein, MoAlg...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2025-06-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923003696 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849246799674474496 |
|---|---|
| author | Shulin Zhang Yu Wang Jinmei Hu Xinyue Cui Xiaoru Kang Wei Zhao Yuemin Pan |
| author_facet | Shulin Zhang Yu Wang Jinmei Hu Xinyue Cui Xiaoru Kang Wei Zhao Yuemin Pan |
| author_sort | Shulin Zhang |
| collection | DOAJ |
| description | Magnaporthe oryzae is the causal agent of rice blast. Glycosylation plays key roles in vegetative growth, development, and infection of M. oryzae. However, several glycosylation-related genes have not been characterized. In this study, we identified a Glyco_transf_22 domain-containing protein, MoAlg9, and found that MoAlg9 is localized to the endoplasmic reticulum (ER). Deletion of MoALG9 significantly affected conidial production, normal appressorium formation, responses to stressors, and pathogenicity of M. oryzae. We also found that the ΔMoalg9 mutant was defective in glycogen utilization, appressorial penetration, and invasive growth in host cells. Moreover, we further demonstrated that MoALG9 regulates the transcription of several target genes involved in conidiation, appressorium formation, and cell wall integrity. In addition, we found that the Glyco_transf_22 domain is essential for normal MoAlg9 function and localization. We also provide evidence that MoAlg9 is involved in N-glycosylation pathway in M. oryzae. Taken together, these results show that MoAlg9 is important for conidiation, appressorium formation, maintenance of cell wall integrity, and the pathogenesis of M. oryzae. |
| format | Article |
| id | doaj-art-4ce0b025ef814a2e8058a932d8d74690 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-4ce0b025ef814a2e8058a932d8d746902025-08-20T03:58:23ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-06-012462266228410.1016/j.jia.2023.10.027The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzaeShulin Zhang0Yu Wang1Jinmei Hu2Xinyue Cui3Xiaoru Kang4Wei Zhao5Yuemin Pan6Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, China; Correspondence Shulin ZhangDepartment of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China; Correspondence Wei ZhaoDepartment of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, China; Correspondence Yuemin PanMagnaporthe oryzae is the causal agent of rice blast. Glycosylation plays key roles in vegetative growth, development, and infection of M. oryzae. However, several glycosylation-related genes have not been characterized. In this study, we identified a Glyco_transf_22 domain-containing protein, MoAlg9, and found that MoAlg9 is localized to the endoplasmic reticulum (ER). Deletion of MoALG9 significantly affected conidial production, normal appressorium formation, responses to stressors, and pathogenicity of M. oryzae. We also found that the ΔMoalg9 mutant was defective in glycogen utilization, appressorial penetration, and invasive growth in host cells. Moreover, we further demonstrated that MoALG9 regulates the transcription of several target genes involved in conidiation, appressorium formation, and cell wall integrity. In addition, we found that the Glyco_transf_22 domain is essential for normal MoAlg9 function and localization. We also provide evidence that MoAlg9 is involved in N-glycosylation pathway in M. oryzae. Taken together, these results show that MoAlg9 is important for conidiation, appressorium formation, maintenance of cell wall integrity, and the pathogenesis of M. oryzae.http://www.sciencedirect.com/science/article/pii/S2095311923003696glycosylationN-mannosyltransferaseAlg9pathogenicityrice blastMagnaporthe oryzae |
| spellingShingle | Shulin Zhang Yu Wang Jinmei Hu Xinyue Cui Xiaoru Kang Wei Zhao Yuemin Pan The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae Journal of Integrative Agriculture glycosylation N-mannosyltransferase Alg9 pathogenicity rice blast Magnaporthe oryzae |
| title | The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae |
| title_full | The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae |
| title_fullStr | The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae |
| title_full_unstemmed | The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae |
| title_short | The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae |
| title_sort | n mannosyltransferase moalg9 plays important roles in the development and pathogenicity of magnaporthe oryzae |
| topic | glycosylation N-mannosyltransferase Alg9 pathogenicity rice blast Magnaporthe oryzae |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923003696 |
| work_keys_str_mv | AT shulinzhang thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT yuwang thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT jinmeihu thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT xinyuecui thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT xiaorukang thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT weizhao thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT yueminpan thenmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT shulinzhang nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT yuwang nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT jinmeihu nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT xinyuecui nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT xiaorukang nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT weizhao nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae AT yueminpan nmannosyltransferasemoalg9playsimportantrolesinthedevelopmentandpathogenicityofmagnaportheoryzae |