Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico
The Trypanosomatidae family encompasses around 24 genera of unicellular protozoans, many of which are transmitted by various hematophagous arthropods, particularly members of the Orders Diptera and Hemiptera. Fleas and ticks—an understudied group of ectoparasites—have been shown to be hosts of a wid...
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2024-12-01
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| author | Héctor M. Zazueta-Islas Beatriz Salceda-Sánchez Herón Huerta-Jiménez Carlos I. Miranda-Caballero Marlene Solis-Cortés Yaretzi de la Cruz-Pacheco Ana Cristina Luquín-García Laura V. Mondragon-Peña Jair Reyes-Hernández José L. Bravo-Ramos María-Guadalupe Sánchez-Otero Javier C. Huerta-Peña Rosa I. Hernández-Herrera Pablo San Martin-del Angel André Luiz Rodrigues Roque Ángel Rodríguez-Moreno Víctor Sánchez-Cordero Héctor Abelardo Rodríguez Martínez Estefania Grostieta Ingeborg Becker Sokani Sánchez-Montes |
| author_facet | Héctor M. Zazueta-Islas Beatriz Salceda-Sánchez Herón Huerta-Jiménez Carlos I. Miranda-Caballero Marlene Solis-Cortés Yaretzi de la Cruz-Pacheco Ana Cristina Luquín-García Laura V. Mondragon-Peña Jair Reyes-Hernández José L. Bravo-Ramos María-Guadalupe Sánchez-Otero Javier C. Huerta-Peña Rosa I. Hernández-Herrera Pablo San Martin-del Angel André Luiz Rodrigues Roque Ángel Rodríguez-Moreno Víctor Sánchez-Cordero Héctor Abelardo Rodríguez Martínez Estefania Grostieta Ingeborg Becker Sokani Sánchez-Montes |
| author_sort | Héctor M. Zazueta-Islas |
| collection | DOAJ |
| description | The Trypanosomatidae family encompasses around 24 genera of unicellular protozoans, many of which are transmitted by various hematophagous arthropods, particularly members of the Orders Diptera and Hemiptera. Fleas and ticks—an understudied group of ectoparasites—have been shown to be hosts of a wide and crescent variety of trypanosomatid species. Further, fleas and ticks of companion animals have been particularly neglected in trypanosomatid surveillance despite the proximity to human populations and the anthropophagous habits of many of these arthropods, which can potentially act as vectors of zoonotic trypanosomatids. We aimed to identify the presence, characterize the species, and establish the prevalence of Kinetoplastids, including members of the Trypanosomatidae family, in ectoparasites collected from dogs and humans from Mexico. A total of 537 ectoparasite specimens belonging to six ectoparasite taxa (<i>Amblyomma mixtum</i>, <i>A. tenellum</i>, <i>Ctenocephalides felis felis</i>, <i>Pulex simulans</i>, <i>Rhipicephalus linnaei</i>, and <i>Rh. sanguineus</i> s.s.) were collected from 15 States of Mexico. An 800 bp fragment of the 18S-rDNA gene from kinetoplastids was amplified and sequenced. The presence of two agents (<i>Trypanosoma caninum</i> and <i>Parabodo</i> sp.) was detected in <i>R. linnaei</i> ticks and one (<i>Blechomonas lauriereadi</i>) in the cat flea <i>Ct. felis felis</i>. This is the first record of genetic material of kinetoplastid species in ectoparasites from dogs and humans in Mexico. |
| format | Article |
| id | doaj-art-4cdd323fa200494fb37aa83e8c916855 |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-4cdd323fa200494fb37aa83e8c9168552025-08-20T02:39:41ZengMDPI AGPathogens2076-08172024-12-011312107210.3390/pathogens13121072Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in MexicoHéctor M. Zazueta-Islas0Beatriz Salceda-Sánchez1Herón Huerta-Jiménez2Carlos I. Miranda-Caballero3Marlene Solis-Cortés4Yaretzi de la Cruz-Pacheco5Ana Cristina Luquín-García6Laura V. Mondragon-Peña7Jair Reyes-Hernández8José L. Bravo-Ramos9María-Guadalupe Sánchez-Otero10Javier C. Huerta-Peña11Rosa I. Hernández-Herrera12Pablo San Martin-del Angel13André Luiz Rodrigues Roque14Ángel Rodríguez-Moreno15Víctor Sánchez-Cordero16Héctor Abelardo Rodríguez Martínez17Estefania Grostieta18Ingeborg Becker19Sokani Sánchez-Montes20Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Entomología, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico City 01480, MexicoLaboratorio de Entomología, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico City 01480, MexicoCentro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoCentro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoFacultad de Bioanálisis, Región Veracruz, Universidad Veracruzana, Veracruz 91700, MexicoFacultad de Bioanálisis, Región Veracruz, Universidad Veracruzana, Veracruz 91700, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoLaboratorio de Biotecnología Ambiental, Facultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoLaboratorio de Biotecnología Ambiental, Facultad de Ciencias Biológicas y Agropecuarias, Región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz 92870, MexicoLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro 21040-900, BrazilLaboratorio de Geografía de la Biodiversidad, Pabellón Nacional de la Biodiversidad Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Geografía de la Biodiversidad, Pabellón Nacional de la Biodiversidad Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Investigaciones Anatomopatológicas, Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoCentro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoCentro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoCentro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoThe Trypanosomatidae family encompasses around 24 genera of unicellular protozoans, many of which are transmitted by various hematophagous arthropods, particularly members of the Orders Diptera and Hemiptera. Fleas and ticks—an understudied group of ectoparasites—have been shown to be hosts of a wide and crescent variety of trypanosomatid species. Further, fleas and ticks of companion animals have been particularly neglected in trypanosomatid surveillance despite the proximity to human populations and the anthropophagous habits of many of these arthropods, which can potentially act as vectors of zoonotic trypanosomatids. We aimed to identify the presence, characterize the species, and establish the prevalence of Kinetoplastids, including members of the Trypanosomatidae family, in ectoparasites collected from dogs and humans from Mexico. A total of 537 ectoparasite specimens belonging to six ectoparasite taxa (<i>Amblyomma mixtum</i>, <i>A. tenellum</i>, <i>Ctenocephalides felis felis</i>, <i>Pulex simulans</i>, <i>Rhipicephalus linnaei</i>, and <i>Rh. sanguineus</i> s.s.) were collected from 15 States of Mexico. An 800 bp fragment of the 18S-rDNA gene from kinetoplastids was amplified and sequenced. The presence of two agents (<i>Trypanosoma caninum</i> and <i>Parabodo</i> sp.) was detected in <i>R. linnaei</i> ticks and one (<i>Blechomonas lauriereadi</i>) in the cat flea <i>Ct. felis felis</i>. This is the first record of genetic material of kinetoplastid species in ectoparasites from dogs and humans in Mexico.https://www.mdpi.com/2076-0817/13/12/1072ectoparasites<i>Rhipicephalus sanguineus</i> s.l.<i>Blechomonas lauriereadi</i><i>Parabodo</i> sp.<i>Trypanosoma caninum</i> |
| spellingShingle | Héctor M. Zazueta-Islas Beatriz Salceda-Sánchez Herón Huerta-Jiménez Carlos I. Miranda-Caballero Marlene Solis-Cortés Yaretzi de la Cruz-Pacheco Ana Cristina Luquín-García Laura V. Mondragon-Peña Jair Reyes-Hernández José L. Bravo-Ramos María-Guadalupe Sánchez-Otero Javier C. Huerta-Peña Rosa I. Hernández-Herrera Pablo San Martin-del Angel André Luiz Rodrigues Roque Ángel Rodríguez-Moreno Víctor Sánchez-Cordero Héctor Abelardo Rodríguez Martínez Estefania Grostieta Ingeborg Becker Sokani Sánchez-Montes Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico Pathogens ectoparasites <i>Rhipicephalus sanguineus</i> s.l. <i>Blechomonas lauriereadi</i> <i>Parabodo</i> sp. <i>Trypanosoma caninum</i> |
| title | Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico |
| title_full | Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico |
| title_fullStr | Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico |
| title_full_unstemmed | Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico |
| title_short | Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico |
| title_sort | molecular detection of kinetoplastid species in ticks and fleas associated with dogs and humans in mexico |
| topic | ectoparasites <i>Rhipicephalus sanguineus</i> s.l. <i>Blechomonas lauriereadi</i> <i>Parabodo</i> sp. <i>Trypanosoma caninum</i> |
| url | https://www.mdpi.com/2076-0817/13/12/1072 |
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