Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation

Apical constriction is a basic mechanism for epithelial morphogenesis, making columnar cells into wedge shape and bending a flat cell sheet. It has long been thought that an apically localized myosin generates a contractile force and drives the cell deformation. However, when we tested the increased...

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Main Authors: Satoshi Yamashita, Shuji Ishihara, François Graner
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-04-01
Series:eLife
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Online Access:https://elifesciences.org/articles/93496
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author Satoshi Yamashita
Shuji Ishihara
François Graner
author_facet Satoshi Yamashita
Shuji Ishihara
François Graner
author_sort Satoshi Yamashita
collection DOAJ
description Apical constriction is a basic mechanism for epithelial morphogenesis, making columnar cells into wedge shape and bending a flat cell sheet. It has long been thought that an apically localized myosin generates a contractile force and drives the cell deformation. However, when we tested the increased apical surface contractility in a cellular Potts model simulation, the constriction increased pressure inside the cell and pushed its lateral surface outward, making the cells adopt a drop shape instead of the expected wedge shape. To keep the lateral surface straight, we considered an alternative model in which the cell shape was determined by cell membrane elasticity and endocytosis, and the increased pressure is balanced among the cells. The cellular Potts model simulation succeeded in reproducing the apical constriction, and it also suggested that a too strong apical surface tension might prevent the tissue invagination.
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spelling doaj-art-4cdc7ecfbb2c447ca9946b536108cb672025-08-20T03:18:05ZengeLife Sciences Publications LtdeLife2050-084X2025-04-011310.7554/eLife.93496Apical constriction requires patterned apical surface remodeling to synchronize cellular deformationSatoshi Yamashita0https://orcid.org/0000-0002-1271-4911Shuji Ishihara1François Graner2Laboratory for Morphogenetic Signaling, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanGraduate School of Arts and Sciences, The University of Tokyo, Tokyo, JapanUniversité Paris Cité, CNRS, MSC, Paris, FranceApical constriction is a basic mechanism for epithelial morphogenesis, making columnar cells into wedge shape and bending a flat cell sheet. It has long been thought that an apically localized myosin generates a contractile force and drives the cell deformation. However, when we tested the increased apical surface contractility in a cellular Potts model simulation, the constriction increased pressure inside the cell and pushed its lateral surface outward, making the cells adopt a drop shape instead of the expected wedge shape. To keep the lateral surface straight, we considered an alternative model in which the cell shape was determined by cell membrane elasticity and endocytosis, and the increased pressure is balanced among the cells. The cellular Potts model simulation succeeded in reproducing the apical constriction, and it also suggested that a too strong apical surface tension might prevent the tissue invagination.https://elifesciences.org/articles/93496apical constrictionsimulationcellular Potts model
spellingShingle Satoshi Yamashita
Shuji Ishihara
François Graner
Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
eLife
apical constriction
simulation
cellular Potts model
title Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
title_full Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
title_fullStr Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
title_full_unstemmed Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
title_short Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
title_sort apical constriction requires patterned apical surface remodeling to synchronize cellular deformation
topic apical constriction
simulation
cellular Potts model
url https://elifesciences.org/articles/93496
work_keys_str_mv AT satoshiyamashita apicalconstrictionrequirespatternedapicalsurfaceremodelingtosynchronizecellulardeformation
AT shujiishihara apicalconstrictionrequirespatternedapicalsurfaceremodelingtosynchronizecellulardeformation
AT francoisgraner apicalconstrictionrequirespatternedapicalsurfaceremodelingtosynchronizecellulardeformation