Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system
Biological control of Striga hermonthica (Del.) Benth. includes push–pull, intercropping using legumes, and soil-borne fungi such as Fusarium oxysporum f.sp. strigae (FOS). Efficacy of both technologies is compromised by various environmental factors. Combining them could potentially overcome their...
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Elsevier
2025-03-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000313 |
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| author | Mekuria Wolde Assena Jochen Schöne Frank Rasche |
| author_facet | Mekuria Wolde Assena Jochen Schöne Frank Rasche |
| author_sort | Mekuria Wolde Assena |
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| description | Biological control of Striga hermonthica (Del.) Benth. includes push–pull, intercropping using legumes, and soil-borne fungi such as Fusarium oxysporum f.sp. strigae (FOS). Efficacy of both technologies is compromised by various environmental factors. Combining them could potentially overcome their limitations and enhance control of S. hermonthica. It was hypothesized that FOS inoculation is compatible with push–pull intercropping, allowing consistent S. hermonthica suppression. Effects of selected phenolic acids and crude root exudates from three legume species (Mucuna pruriens, Desmodium uncinatum, Crotalaria juncea) on FOS mycelial growth and spore germination were studied. Bioassays showed that most phenolic acids (e.g., caffeic, p-hydroxybenzoic, syringic) had no effect on FOS development, except for piperonylic (80 %, 38 %) and t-cinnamic (53 %, 33 %) acids, which strongly inhibited growth at concentrations of 1 and 0.5 mM, respectively. Crude root exudates from Crotalaria inhibited FOS mycelial growth at 1 (44 %) and 0.5 (32 %) mg ml-1. Desmodium and Mucuna exudates showed no inhibition. Greenhouse results showed that legume intercropping and FOS inoculation reduced S. hermonthica emergence, with their integration achieving over 95 % suppression and boosting maize biomass. Legume presence enhanced FOS proliferation, with Mucuna and Desmodium increasing FOS gene copy numbers by over 44 %. LC-MS analysis identified nine phenolic acids, with p-coumaric (85–128 µg g−1) and p-hydroxybenzoic (50–97 µg g−1) acid being most abundant; the latter positively correlated with FOS abundance. In this setting, legume intercropping with maize did not impair FOS proliferation in rhizosphere soil and effectively suppressed S. hermonthica, demonstrating compatibility of FOS inoculation with push–pull for effective S. hermonthica management. |
| format | Article |
| id | doaj-art-4cdabe5b5e7841d2ad58506489892487 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Biological Control |
| spelling | doaj-art-4cdabe5b5e7841d2ad585064898924872025-02-09T04:59:45ZengElsevierBiological Control1049-96442025-03-01202105721Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull systemMekuria Wolde Assena0Jochen Schöne1Frank Rasche2Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), University of Hohenheim, Garbenstr. 13, 70599 Stuttgart, Germany; Department of Horticulture, Wolkite University, Wolkite, EthiopiaInstitute of Phytomedicine, University of Hohenheim, Otto-Sander-Str. 5, 70599 Stuttgart, GermanyInstitute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), University of Hohenheim, Garbenstr. 13, 70599 Stuttgart, Germany; International Institute of Tropical Agriculture, P.O. Box 30772-00100, Nairobi, Kenya1; Corresponding author at: Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), University of Hohenheim, Garbenstr. 13, 70599 Stuttgart, Germany.Biological control of Striga hermonthica (Del.) Benth. includes push–pull, intercropping using legumes, and soil-borne fungi such as Fusarium oxysporum f.sp. strigae (FOS). Efficacy of both technologies is compromised by various environmental factors. Combining them could potentially overcome their limitations and enhance control of S. hermonthica. It was hypothesized that FOS inoculation is compatible with push–pull intercropping, allowing consistent S. hermonthica suppression. Effects of selected phenolic acids and crude root exudates from three legume species (Mucuna pruriens, Desmodium uncinatum, Crotalaria juncea) on FOS mycelial growth and spore germination were studied. Bioassays showed that most phenolic acids (e.g., caffeic, p-hydroxybenzoic, syringic) had no effect on FOS development, except for piperonylic (80 %, 38 %) and t-cinnamic (53 %, 33 %) acids, which strongly inhibited growth at concentrations of 1 and 0.5 mM, respectively. Crude root exudates from Crotalaria inhibited FOS mycelial growth at 1 (44 %) and 0.5 (32 %) mg ml-1. Desmodium and Mucuna exudates showed no inhibition. Greenhouse results showed that legume intercropping and FOS inoculation reduced S. hermonthica emergence, with their integration achieving over 95 % suppression and boosting maize biomass. Legume presence enhanced FOS proliferation, with Mucuna and Desmodium increasing FOS gene copy numbers by over 44 %. LC-MS analysis identified nine phenolic acids, with p-coumaric (85–128 µg g−1) and p-hydroxybenzoic (50–97 µg g−1) acid being most abundant; the latter positively correlated with FOS abundance. In this setting, legume intercropping with maize did not impair FOS proliferation in rhizosphere soil and effectively suppressed S. hermonthica, demonstrating compatibility of FOS inoculation with push–pull for effective S. hermonthica management.http://www.sciencedirect.com/science/article/pii/S1049964425000313Striga hermonthicaIntegrated Striga managementFusarium oxysporum f.sp. strigaeFOSDesmodium uncinatumPiperonylic acid |
| spellingShingle | Mekuria Wolde Assena Jochen Schöne Frank Rasche Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system Biological Control Striga hermonthica Integrated Striga management Fusarium oxysporum f.sp. strigae FOS Desmodium uncinatum Piperonylic acid |
| title | Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system |
| title_full | Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system |
| title_fullStr | Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system |
| title_full_unstemmed | Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system |
| title_short | Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system |
| title_sort | legume derived phenolic acids influence fusarium oxysporum f sp strigae compatibility and biocontrol potential in a striga push pull system |
| topic | Striga hermonthica Integrated Striga management Fusarium oxysporum f.sp. strigae FOS Desmodium uncinatum Piperonylic acid |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000313 |
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