Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms
Abstract Utilizing phytohormones—brassinosteroids (BRs), jasmonic acid (JA), and strigolactones (SLs) presents a novel approach to overcome toxicity of arsenic (As) in agricultural settings. The present study investigates the role of three phytohormones BRs, JA, and SLs in ameliorating As-induced st...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-11629-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849333332643414016 |
|---|---|
| author | Hiba Shaghaleh Yousef Alhaj Hamoud Muhammad Hamzah Saleem Hosam O. Elansary Eman A. Mahmoud Mohamed S. Sheteiwy |
| author_facet | Hiba Shaghaleh Yousef Alhaj Hamoud Muhammad Hamzah Saleem Hosam O. Elansary Eman A. Mahmoud Mohamed S. Sheteiwy |
| author_sort | Hiba Shaghaleh |
| collection | DOAJ |
| description | Abstract Utilizing phytohormones—brassinosteroids (BRs), jasmonic acid (JA), and strigolactones (SLs) presents a novel approach to overcome toxicity of arsenic (As) in agricultural settings. The present study investigates the role of three phytohormones BRs, JA, and SLs in ameliorating As-induced stressed in Oryza sativa L. (rice) seedlings. s In the present study, we have used different levels of BRs (0.1 µM), JA (1 µM), and SLs (0.05 µM) every 7 days to O. sativa seedlings when exposed to different levels of As levels of 100 mg−1 kg and 200 mg kg−1 (as sodium arsenate) to examine various growth and biochemical parameters in O. sativa. The results showed that the As toxicity in the soil showed a significantly (P < 0.05) declined in the growth, gas exchange attributes, sugars, AsA-GSH cycle, cellular fractionation, proline metabolism in O. sativa. However, As toxicity significantly (P < 0.05) increased oxidative stress biomarkers, enzymatic and nonenzymatic antioxidants including their gene expression in O. sativa. Although, the application of phytohormones including BRs, JA, and SLs showed a significant (P < 0.05) increase in the plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds and their gene expression and also decreased the oxidative stress, and As uptake. In addition, individual or combined application of phytohormones including BRs, JA, and SLs enhanced the cellular fractionation and decreases the proline metabolism and AsA − GSH cycle in O. sativa. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal contamination in agricultural soils. |
| format | Article |
| id | doaj-art-4cd9b3dfd84848248b21ae951ae70012 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4cd9b3dfd84848248b21ae951ae700122025-08-20T03:45:53ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-11629-zPhytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanismsHiba Shaghaleh0Yousef Alhaj Hamoud1Muhammad Hamzah Saleem2Hosam O. Elansary3Eman A. Mahmoud4Mohamed S. Sheteiwy5School of Energy and Environment, Southeast UniversityCollege of Hydrology and Water Recourses, Hohai UniversityOffice of Academic Research, Office of VP for Research & Graduate Studies, Qatar UniversityDepartment of Plant Production, College of Food and Agriculture Sciences, King Saud UniversityDepartment of Food Science College of Agriculture , Eman A. Mahmoud Damietta University Department of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates UniversityAbstract Utilizing phytohormones—brassinosteroids (BRs), jasmonic acid (JA), and strigolactones (SLs) presents a novel approach to overcome toxicity of arsenic (As) in agricultural settings. The present study investigates the role of three phytohormones BRs, JA, and SLs in ameliorating As-induced stressed in Oryza sativa L. (rice) seedlings. s In the present study, we have used different levels of BRs (0.1 µM), JA (1 µM), and SLs (0.05 µM) every 7 days to O. sativa seedlings when exposed to different levels of As levels of 100 mg−1 kg and 200 mg kg−1 (as sodium arsenate) to examine various growth and biochemical parameters in O. sativa. The results showed that the As toxicity in the soil showed a significantly (P < 0.05) declined in the growth, gas exchange attributes, sugars, AsA-GSH cycle, cellular fractionation, proline metabolism in O. sativa. However, As toxicity significantly (P < 0.05) increased oxidative stress biomarkers, enzymatic and nonenzymatic antioxidants including their gene expression in O. sativa. Although, the application of phytohormones including BRs, JA, and SLs showed a significant (P < 0.05) increase in the plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds and their gene expression and also decreased the oxidative stress, and As uptake. In addition, individual or combined application of phytohormones including BRs, JA, and SLs enhanced the cellular fractionation and decreases the proline metabolism and AsA − GSH cycle in O. sativa. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal contamination in agricultural soils.https://doi.org/10.1038/s41598-025-11629-zMetal accumulationPhytohormonesCereal cropGene expressionProline |
| spellingShingle | Hiba Shaghaleh Yousef Alhaj Hamoud Muhammad Hamzah Saleem Hosam O. Elansary Eman A. Mahmoud Mohamed S. Sheteiwy Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms Scientific Reports Metal accumulation Phytohormones Cereal crop Gene expression Proline |
| title | Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms |
| title_full | Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms |
| title_fullStr | Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms |
| title_full_unstemmed | Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms |
| title_short | Phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses, proline metabolism, and arsenic detoxification mechanisms |
| title_sort | phytohormonal modulation alleviates arsenic toxicity in rice by enhancing antioxidant defenses proline metabolism and arsenic detoxification mechanisms |
| topic | Metal accumulation Phytohormones Cereal crop Gene expression Proline |
| url | https://doi.org/10.1038/s41598-025-11629-z |
| work_keys_str_mv | AT hibashaghaleh phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms AT yousefalhajhamoud phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms AT muhammadhamzahsaleem phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms AT hosamoelansary phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms AT emanamahmoud phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms AT mohamedssheteiwy phytohormonalmodulationalleviatesarsenictoxicityinricebyenhancingantioxidantdefensesprolinemetabolismandarsenicdetoxificationmechanisms |