Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.

The fidelity of DNA replication requires an appropriate balance of dNTPs, yet the nascent leading and lagging strands of the nuclear genome are primarily synthesized by replicases that differ in subunit composition, protein partnerships and biochemical properties, including fidelity. These facts pos...

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Main Authors: Robert J Buckland, Danielle L Watt, Balasubramanyam Chittoor, Anna Karin Nilsson, Thomas A Kunkel, Andrei Chabes
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-12-01
Series:PLoS Genetics
Online Access:https://doi.org/10.1371/journal.pgen.1004846
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author Robert J Buckland
Danielle L Watt
Balasubramanyam Chittoor
Anna Karin Nilsson
Thomas A Kunkel
Andrei Chabes
author_facet Robert J Buckland
Danielle L Watt
Balasubramanyam Chittoor
Anna Karin Nilsson
Thomas A Kunkel
Andrei Chabes
author_sort Robert J Buckland
collection DOAJ
description The fidelity of DNA replication requires an appropriate balance of dNTPs, yet the nascent leading and lagging strands of the nuclear genome are primarily synthesized by replicases that differ in subunit composition, protein partnerships and biochemical properties, including fidelity. These facts pose the question of whether imbalanced dNTP pools differentially influence leading and lagging strand replication fidelity. Here we test this possibility by examining strand-specific replication infidelity driven by a mutation in yeast ribonucleotide reductase, rnr1-Y285A, that leads to elevated dTTP and dCTP concentrations. The results for the CAN1 mutational reporter gene present in opposite orientations in the genome reveal that the rates, and surprisingly even the sequence contexts, of replication errors are remarkably similar for leading and lagging strand synthesis. Moreover, while many mismatches driven by the dNTP pool imbalance are efficiently corrected by mismatch repair, others are repaired less efficiently, especially those in sequence contexts suggesting reduced proofreading due to increased mismatch extension driven by the high dTTP and dCTP concentrations. Thus the two DNA strands of the nuclear genome are at similar risk of mutations resulting from this dNTP pool imbalance, and this risk is not completely suppressed even when both major replication error correction mechanisms are genetically intact.
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publishDate 2014-12-01
publisher Public Library of Science (PLoS)
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spelling doaj-art-4ccd5cff9c9f41b1864a275563c414882025-08-20T03:10:07ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-12-011012e100484610.1371/journal.pgen.1004846Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.Robert J BucklandDanielle L WattBalasubramanyam ChittoorAnna Karin NilssonThomas A KunkelAndrei ChabesThe fidelity of DNA replication requires an appropriate balance of dNTPs, yet the nascent leading and lagging strands of the nuclear genome are primarily synthesized by replicases that differ in subunit composition, protein partnerships and biochemical properties, including fidelity. These facts pose the question of whether imbalanced dNTP pools differentially influence leading and lagging strand replication fidelity. Here we test this possibility by examining strand-specific replication infidelity driven by a mutation in yeast ribonucleotide reductase, rnr1-Y285A, that leads to elevated dTTP and dCTP concentrations. The results for the CAN1 mutational reporter gene present in opposite orientations in the genome reveal that the rates, and surprisingly even the sequence contexts, of replication errors are remarkably similar for leading and lagging strand synthesis. Moreover, while many mismatches driven by the dNTP pool imbalance are efficiently corrected by mismatch repair, others are repaired less efficiently, especially those in sequence contexts suggesting reduced proofreading due to increased mismatch extension driven by the high dTTP and dCTP concentrations. Thus the two DNA strands of the nuclear genome are at similar risk of mutations resulting from this dNTP pool imbalance, and this risk is not completely suppressed even when both major replication error correction mechanisms are genetically intact.https://doi.org/10.1371/journal.pgen.1004846
spellingShingle Robert J Buckland
Danielle L Watt
Balasubramanyam Chittoor
Anna Karin Nilsson
Thomas A Kunkel
Andrei Chabes
Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
PLoS Genetics
title Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
title_full Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
title_fullStr Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
title_full_unstemmed Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
title_short Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.
title_sort increased and imbalanced dntp pools symmetrically promote both leading and lagging strand replication infidelity
url https://doi.org/10.1371/journal.pgen.1004846
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