Investigation of the in-vitro effect of lactobacillus acidophilus and lactobacillus casei on biofilm genes of methicillin-resistant and methicillin-sensitive staphylococcus aureus
<b>Aim: </b>Staphylococcus aureuss biofilm ability which control via icaA and icaR gene is a serious problem in the treatment of infections. Aim of this study was to investigate in-vitro effects of important probiotic L. acidophilus and L. casei on the icaA and icaR genes expressions of...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1240 |
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| Summary: | <b>Aim: </b>Staphylococcus aureuss biofilm ability which control via
icaA and icaR gene is a serious problem in the treatment of infections.
Aim of this study was to investigate in-vitro effects of
important probiotic L. acidophilus and L. casei on the icaA and
icaR genes expressions of the MRSA and MSSA strains.<p>
<b>Materials and Methods:</b> In this study, to determine the icaA
and icaR gene expressions, 6 tubes in probiotics were prepared.
Incubated at 37°C in 5% CO2 medium. RNA isolations were
performed at the 6th, 12th, and 24th hours of incubation. After
cDNA synthesis, real-time PCR with primers specific for icaA,
icaR and 16S rRNA genes were studied. The results were calculated
with delta delta Ct method.<p>
<b>Results: </b>S. aureus ATCC 43300 (MRSA) and S. aureus ATCC
29213 (MSSA) icaA gene and icaR gene expressions showed
down regulation and upregulation respectively after encountering
L. acidophilus and L. casei. The icaA and icaR gene expression
ratios determined at 12 hours and 24 hours were found to be
similar in both MRSA and MSSA strains.<p>
<b>Conclusion: </b>This study was the first study to investigate effect
of L. acidophilus and L. casei strains on biofilm gene expression
of the S. aureus strains by the real-time PCR method. It was determined
that L. acidophilus and L. casei were able to alter icaA
and icaR gene expression by affecting the MRSA and MSSA strains
in-vitro. We believe that the antibiotic properties of these
probiotic strains may be a new treatment option against S. aureus
biofilm infections. |
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| ISSN: | 1309-6958 2146-1953 |