Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection
The article presents an optimized method for the determination of the ratio of 8-oxo-2'-deoxyguanosine (formed in DNA due to the action of active forms of oxygen) to 2'-deoxyguanosine. The ratio was determined by reverse phase HPLC combined with amperometric detection. It was shown that th...
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2010-06-01
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| Series: | Тонкие химические технологии |
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| Online Access: | https://www.finechem-mirea.ru/jour/article/view/976 |
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| author | D. S. Esipov E. V. Sidorenko O. V. Esipova T. A. Gorbacheva T. S. Nevredimova A. L. Krushinskiy V. S. Kuzenkov V. P. Reutov |
| author_facet | D. S. Esipov E. V. Sidorenko O. V. Esipova T. A. Gorbacheva T. S. Nevredimova A. L. Krushinskiy V. S. Kuzenkov V. P. Reutov |
| author_sort | D. S. Esipov |
| collection | DOAJ |
| description | The article presents an optimized method for the determination of the ratio of 8-oxo-2'-deoxyguanosine (formed in DNA due to the action of active forms of oxygen) to 2'-deoxyguanosine. The ratio was determined by reverse phase HPLC combined with amperometric detection. It was shown that the ratio increases upon oxidative stress caused by the action of large doses of ascorbic acid in samples of DNA isolated from the liver of experimental rats. |
| format | Article |
| id | doaj-art-4ccc9a49c4a6481489d800c471bd4881 |
| institution | DOAJ |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| publishDate | 2010-06-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj-art-4ccc9a49c4a6481489d800c471bd48812025-08-20T03:23:19ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752010-06-01536974970Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detectionD. S. Esipov0E. V. Sidorenko1O. V. Esipova2T. A. Gorbacheva3T. S. Nevredimova4A. L. Krushinskiy5V. S. Kuzenkov6V. P. Reutov7МГУ им. М.В. ЛомоносоваМГУ им. М.В. ЛомоносоваM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571МГУ им. М.В. ЛомоносоваМГУ им. М.В. ЛомоносоваИнститут высшей нервной деятельности и нейрофизиологии РАНThe article presents an optimized method for the determination of the ratio of 8-oxo-2'-deoxyguanosine (formed in DNA due to the action of active forms of oxygen) to 2'-deoxyguanosine. The ratio was determined by reverse phase HPLC combined with amperometric detection. It was shown that the ratio increases upon oxidative stress caused by the action of large doses of ascorbic acid in samples of DNA isolated from the liver of experimental rats.https://www.finechem-mirea.ru/jour/article/view/9768-oxo-2'-deoxyguanosineoxidative stressascorbic acidreverse phase hplcamperometric detection |
| spellingShingle | D. S. Esipov E. V. Sidorenko O. V. Esipova T. A. Gorbacheva T. S. Nevredimova A. L. Krushinskiy V. S. Kuzenkov V. P. Reutov Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection Тонкие химические технологии 8-oxo-2'-deoxyguanosine oxidative stress ascorbic acid reverse phase hplc amperometric detection |
| title | Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection |
| title_full | Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection |
| title_fullStr | Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection |
| title_full_unstemmed | Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection |
| title_short | Determining 8-oxo-2’-deoxyguanosine/2’-deoxyguanosine ratio in DNA by reverse phase HPLC combined with amperometric detection |
| title_sort | determining 8 oxo 2 deoxyguanosine 2 deoxyguanosine ratio in dna by reverse phase hplc combined with amperometric detection |
| topic | 8-oxo-2'-deoxyguanosine oxidative stress ascorbic acid reverse phase hplc amperometric detection |
| url | https://www.finechem-mirea.ru/jour/article/view/976 |
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