Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases
Objective: We describe four patients presenting early oral cancers, detected cytologically on non‐invasive brush biopsies including DNA‐image cytometry as an adjunctive method before histology on scalpel biopsies confirmed the evidence of malignancy. Methods: Brush biopsies were performed and smears...
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2003/305151 |
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| _version_ | 1832564256725270528 |
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| author | Torsten W. Remmerbach Horst Weidenbach Alexander Hemprich Alfred Böcking |
| author_facet | Torsten W. Remmerbach Horst Weidenbach Alexander Hemprich Alfred Böcking |
| author_sort | Torsten W. Remmerbach |
| collection | DOAJ |
| description | Objective: We describe four patients presenting early oral cancers, detected cytologically on non‐invasive brush biopsies including DNA‐image cytometry as an adjunctive method before histology on scalpel biopsies confirmed the evidence of malignancy. Methods: Brush biopsies were performed and smears thereof investigated cytologically. After Feulgen restaining, DNA‐measurements were performed using a DNA‐Image‐Cytometer. Case reports: Oral squamous cell carcinomas were diagnosed cytologically in macroscopically suspicious lesions and malignancy confirmed by DNA‐cytometry. The initially performed scalpel biopsies did neither supply evidence of oral cancer nor of severe dysplasia. After at least one to 15 months the occurrence of cancer was finally proven histologically on a second scalpel biopsy each (three microinvasive and one in situ carcinoma). Conclusion: Non‐invasive brush biopsies are a suitable instrument for early cytologic detection of cancer of the mouth. DNA‐image‐cytometry, as an adjunctive method, can be used to confirm the cytologic diagnosis or suspicion of cancer in patients with doubtful lesions (dysplasias). DNA‐aneuploidy is a marker for (prospective) malignancy in smears of the oral cavity, which may detect malignancy months prior to histology. In future this method could be used as a mass screening tool in dentists practise. Colour figures can be viewed on http://www.esacp.org/acp/2003/25‐4/remmerbach.htm. |
| format | Article |
| id | doaj-art-4cbb0dc7f22741a6af1bdb2473b62a6a |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-4cbb0dc7f22741a6af1bdb2473b62a6a2025-02-03T01:11:25ZengWileyAnalytical Cellular Pathology0921-89121878-36512003-01-0125415916610.1155/2003/305151Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four CasesTorsten W. Remmerbach0Horst Weidenbach1Alexander Hemprich2Alfred Böcking3Department of Oral, Maxillofacial and Facial Plastic Surgery, University of Leipzig, Nürnberger Straße 57, D‐04103 Leipzig, GermanyInstitute of Pathology, University of Leipzig, Liebigstraße 26, D‐04103 Leipzig, GermanyDepartment of Oral, Maxillofacial and Facial Plastic Surgery, University of Leipzig, Nürnberger Straße 57, D‐04103 Leipzig, GermanyInstitute of Cytopathology, Heinrich Heine University, Moorenstraße 5, D‐40225 Düsseldorf, GermanyObjective: We describe four patients presenting early oral cancers, detected cytologically on non‐invasive brush biopsies including DNA‐image cytometry as an adjunctive method before histology on scalpel biopsies confirmed the evidence of malignancy. Methods: Brush biopsies were performed and smears thereof investigated cytologically. After Feulgen restaining, DNA‐measurements were performed using a DNA‐Image‐Cytometer. Case reports: Oral squamous cell carcinomas were diagnosed cytologically in macroscopically suspicious lesions and malignancy confirmed by DNA‐cytometry. The initially performed scalpel biopsies did neither supply evidence of oral cancer nor of severe dysplasia. After at least one to 15 months the occurrence of cancer was finally proven histologically on a second scalpel biopsy each (three microinvasive and one in situ carcinoma). Conclusion: Non‐invasive brush biopsies are a suitable instrument for early cytologic detection of cancer of the mouth. DNA‐image‐cytometry, as an adjunctive method, can be used to confirm the cytologic diagnosis or suspicion of cancer in patients with doubtful lesions (dysplasias). DNA‐aneuploidy is a marker for (prospective) malignancy in smears of the oral cavity, which may detect malignancy months prior to histology. In future this method could be used as a mass screening tool in dentists practise. Colour figures can be viewed on http://www.esacp.org/acp/2003/25‐4/remmerbach.htm.http://dx.doi.org/10.1155/2003/305151 |
| spellingShingle | Torsten W. Remmerbach Horst Weidenbach Alexander Hemprich Alfred Böcking Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases Analytical Cellular Pathology |
| title | Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases |
| title_full | Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases |
| title_fullStr | Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases |
| title_full_unstemmed | Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases |
| title_short | Earliest Detection of Oral Cancer Using Non-Invasive Brush Biopsy Including DNA-Image-Cytometry: Report on Four Cases |
| title_sort | earliest detection of oral cancer using non invasive brush biopsy including dna image cytometry report on four cases |
| url | http://dx.doi.org/10.1155/2003/305151 |
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