Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector
The expressive characteristics of LeETR2 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect analysis （RPA） technique. The results showed that LeETR2 mRNA expressed in all the tissues examined, and it's expression was unaffected by the endogenous ethylene in le...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2001-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.02.0156 |
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| _version_ | 1849247437845168128 |
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| author | ZHENG Tie-song YING Tie-jin ZENG Guang-wen HE Guo-qing |
| author_facet | ZHENG Tie-song YING Tie-jin ZENG Guang-wen HE Guo-qing |
| author_sort | ZHENG Tie-song |
| collection | DOAJ |
| description | The expressive characteristics of LeETR2 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect analysis （RPA） technique. The results showed that LeETR2 mRNA expressed in all the tissues examined, and it's expression was unaffected by the endogenous ethylene in leaves. However, LeETE2 mRNA was developmentally regulated, and partially induced by ethylene in fruits. The results indicated that LeETR2 mRNA exhibits different expression pattern from other ethylene receptor genes in tomato. Moreover, LeETR2 antisense gene expression vector has been constructed for further research. |
| format | Article |
| id | doaj-art-4cb4ce63ce854a0e8aa3ce6b8ee50176 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2001-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-4cb4ce63ce854a0e8aa3ce6b8ee501762025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552001-03-012715616010.3785/1008-9209.2001.02.015610089209Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vectorZHENG Tie-songYING Tie-jinZENG Guang-wenHE Guo-qingThe expressive characteristics of LeETR2 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect analysis （RPA） technique. The results showed that LeETR2 mRNA expressed in all the tissues examined, and it's expression was unaffected by the endogenous ethylene in leaves. However, LeETE2 mRNA was developmentally regulated, and partially induced by ethylene in fruits. The results indicated that LeETR2 mRNA exhibits different expression pattern from other ethylene receptor genes in tomato. Moreover, LeETR2 antisense gene expression vector has been constructed for further research.https://www.academax.com/doi/10.3785/1008-9209.2001.02.0156Ethylene receptorLeETR2Antisense geneRPATomato |
| spellingShingle | ZHENG Tie-song YING Tie-jin ZENG Guang-wen HE Guo-qing Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector 浙江大学学报. 农业与生命科学版 Ethylene receptor LeETR2 Antisense gene RPA Tomato |
| title | Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector |
| title_full | Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector |
| title_fullStr | Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector |
| title_full_unstemmed | Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector |
| title_short | Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector |
| title_sort | expression of ethylene receptor gene leetr2 in tomato mutant epi and construction of its antisense expression vector |
| topic | Ethylene receptor LeETR2 Antisense gene RPA Tomato |
| url | https://www.academax.com/doi/10.3785/1008-9209.2001.02.0156 |
| work_keys_str_mv | AT zhengtiesong expressionofethylenereceptorgeneleetr2intomatomutantepiandconstructionofitsantisenseexpressionvector AT yingtiejin expressionofethylenereceptorgeneleetr2intomatomutantepiandconstructionofitsantisenseexpressionvector AT zengguangwen expressionofethylenereceptorgeneleetr2intomatomutantepiandconstructionofitsantisenseexpressionvector AT heguoqing expressionofethylenereceptorgeneleetr2intomatomutantepiandconstructionofitsantisenseexpressionvector |