The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study

Abstract To investigate the causal relationship between oral infectious diseases and lacunar stroke (LS) and to identify the role of interleukin-1α (IL-1α), interleukin-6 (IL-6), tumor necrosis factor (TNF), and C-reaction protein (CRP) as potential mediators. The meta-analysis incorporating cross-s...

Full description

Saved in:
Bibliographic Details
Main Authors: Qifan Zheng, Tong Xu, Shiyin Luo, Jiahui Zhao, Yue Ying, Nan Liu, Aijia Jiang, Yaping Pan, Dongmei Zhang
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-99742-x
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849726244649697280
author Qifan Zheng
Tong Xu
Shiyin Luo
Jiahui Zhao
Yue Ying
Nan Liu
Aijia Jiang
Yaping Pan
Dongmei Zhang
author_facet Qifan Zheng
Tong Xu
Shiyin Luo
Jiahui Zhao
Yue Ying
Nan Liu
Aijia Jiang
Yaping Pan
Dongmei Zhang
author_sort Qifan Zheng
collection DOAJ
description Abstract To investigate the causal relationship between oral infectious diseases and lacunar stroke (LS) and to identify the role of interleukin-1α (IL-1α), interleukin-6 (IL-6), tumor necrosis factor (TNF), and C-reaction protein (CRP) as potential mediators. The meta-analysis incorporating cross-sectional studies was carried out. Additionally, two-sample Mendelian randomization (MR) analysis was performed to explore the associations between genetically predicted oral infectious diseases (including dental caries, periodontitis, and pulp and periapical diseases) and lacunar stroke, utilizing summary-level data from genome-wide association studies (GWAS). This was followed by a mediation analysis to explore the role of IL-1α, IL-6, TNF, and CRP. Meta-analysis suggested that individuals with periodontitis have a 5.16 times higher risk of developing LS compared to those without periodontitis (95%CI 3.68–7.24). Genetically predicted pulp and periapical diseases (Odds ratios [OR]: 1.20, 95% CI 1.03–1.41) and periodontitis (OR: 1.24, 95%CI 1.03–1.49) showed a moderate association with LS. However, the mediation analysis yielded negative results. The evidence derived from both the MR study and the meta-analysis suggested a potential association between periodontitis and LS. These findings indicated that periodontitis might play a role in the development of LS. However, given the limitations inherent in our research, further studies are necessary to validate these conclusions.
format Article
id doaj-art-4cabf13211f74cc6a341e7ddf6caeda2
institution DOAJ
issn 2045-2322
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-4cabf13211f74cc6a341e7ddf6caeda22025-08-20T03:10:14ZengNature PortfolioScientific Reports2045-23222025-05-0115111210.1038/s41598-025-99742-xThe contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization studyQifan Zheng0Tong Xu1Shiyin Luo2Jiahui Zhao3Yue Ying4Nan Liu5Aijia Jiang6Yaping Pan7Dongmei Zhang8Department of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Oral Mucosiology, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityDepartment of Periodontics, Liaoning Provincial Key Laboratory of Oral Disease, School and Hospital of Stomatology, China Medical UniversityAbstract To investigate the causal relationship between oral infectious diseases and lacunar stroke (LS) and to identify the role of interleukin-1α (IL-1α), interleukin-6 (IL-6), tumor necrosis factor (TNF), and C-reaction protein (CRP) as potential mediators. The meta-analysis incorporating cross-sectional studies was carried out. Additionally, two-sample Mendelian randomization (MR) analysis was performed to explore the associations between genetically predicted oral infectious diseases (including dental caries, periodontitis, and pulp and periapical diseases) and lacunar stroke, utilizing summary-level data from genome-wide association studies (GWAS). This was followed by a mediation analysis to explore the role of IL-1α, IL-6, TNF, and CRP. Meta-analysis suggested that individuals with periodontitis have a 5.16 times higher risk of developing LS compared to those without periodontitis (95%CI 3.68–7.24). Genetically predicted pulp and periapical diseases (Odds ratios [OR]: 1.20, 95% CI 1.03–1.41) and periodontitis (OR: 1.24, 95%CI 1.03–1.49) showed a moderate association with LS. However, the mediation analysis yielded negative results. The evidence derived from both the MR study and the meta-analysis suggested a potential association between periodontitis and LS. These findings indicated that periodontitis might play a role in the development of LS. However, given the limitations inherent in our research, further studies are necessary to validate these conclusions.https://doi.org/10.1038/s41598-025-99742-xOral infectious diseasesPeriodontitisLacunar strokeMendelian randomization analysisMeta-analysis
spellingShingle Qifan Zheng
Tong Xu
Shiyin Luo
Jiahui Zhao
Yue Ying
Nan Liu
Aijia Jiang
Yaping Pan
Dongmei Zhang
The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
Scientific Reports
Oral infectious diseases
Periodontitis
Lacunar stroke
Mendelian randomization analysis
Meta-analysis
title The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
title_full The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
title_fullStr The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
title_full_unstemmed The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
title_short The contribution of oral infectious diseases in lacunar stroke based on meta-analysis and Mendelian randomization study
title_sort contribution of oral infectious diseases in lacunar stroke based on meta analysis and mendelian randomization study
topic Oral infectious diseases
Periodontitis
Lacunar stroke
Mendelian randomization analysis
Meta-analysis
url https://doi.org/10.1038/s41598-025-99742-x
work_keys_str_mv AT qifanzheng thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT tongxu thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT shiyinluo thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT jiahuizhao thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT yueying thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT nanliu thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT aijiajiang thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT yapingpan thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT dongmeizhang thecontributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT qifanzheng contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT tongxu contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT shiyinluo contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT jiahuizhao contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT yueying contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT nanliu contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT aijiajiang contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT yapingpan contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy
AT dongmeizhang contributionoforalinfectiousdiseasesinlacunarstrokebasedonmetaanalysisandmendelianrandomizationstudy