A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis
Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells (SSCs). The mechanisms underlying the transition from SSCs to sperm are largely unknown. Most studies utilize gene knockout mice to explain the mechanisms. However, the productio...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-03-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | https://journals.lww.com/10.4103/aja202453 |
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| author | Shan Li Ying Yuan Ke-Yu Zhang Yi-Dan Guo Lu-Tong Wang Xiao-Yuan Zhang Shu Zhang Qi Yan Rong Zhang Jie Chen Feng-Tang Yang Jing-Rui Li |
| author_facet | Shan Li Ying Yuan Ke-Yu Zhang Yi-Dan Guo Lu-Tong Wang Xiao-Yuan Zhang Shu Zhang Qi Yan Rong Zhang Jie Chen Feng-Tang Yang Jing-Rui Li |
| author_sort | Shan Li |
| collection | DOAJ |
| description | Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells (SSCs). The mechanisms underlying the transition from SSCs to sperm are largely unknown. Most studies utilize gene knockout mice to explain the mechanisms. However, the production of genetically engineered mice is costly and time-consuming. In this study, we presented a convenient research strategy using an RNA interference (RNAi) and testicular transplantation approach. Histone H3 lysine 9 (H3K9) methylation was dynamically regulated during spermatogenesis. As Jumonji domain-containing protein 1A (JMJD1A) and Jumonji domain-containing protein 2C (JMJD2C) demethylases catalyze histone H3 lysine 9 dimethylation (H3K9me2), we firstly analyzed the expression profile of the two demethylases and then investigated their function. Using the convenient research strategy, we showed that normal spermatogenesis is disrupted due to the downregulated expression of both demethylases. These results suggest that this strategy might be a simple and alternative approach for analyzing spermatogenesis relative to the gene knockout mice strategy. |
| format | Article |
| id | doaj-art-4cab749265a84d41b7b1d08df0369925 |
| institution | OA Journals |
| issn | 1008-682X 1745-7262 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj-art-4cab749265a84d41b7b1d08df03699252025-08-20T02:05:16ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622025-03-0127226126710.4103/aja202453A convenient research strategy for functional verification of epigenetic regulators during spermatogenesisShan LiYing YuanKe-Yu ZhangYi-Dan GuoLu-Tong WangXiao-Yuan ZhangShu ZhangQi YanRong ZhangJie ChenFeng-Tang YangJing-Rui LiSpermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells (SSCs). The mechanisms underlying the transition from SSCs to sperm are largely unknown. Most studies utilize gene knockout mice to explain the mechanisms. However, the production of genetically engineered mice is costly and time-consuming. In this study, we presented a convenient research strategy using an RNA interference (RNAi) and testicular transplantation approach. Histone H3 lysine 9 (H3K9) methylation was dynamically regulated during spermatogenesis. As Jumonji domain-containing protein 1A (JMJD1A) and Jumonji domain-containing protein 2C (JMJD2C) demethylases catalyze histone H3 lysine 9 dimethylation (H3K9me2), we firstly analyzed the expression profile of the two demethylases and then investigated their function. Using the convenient research strategy, we showed that normal spermatogenesis is disrupted due to the downregulated expression of both demethylases. These results suggest that this strategy might be a simple and alternative approach for analyzing spermatogenesis relative to the gene knockout mice strategy.https://journals.lww.com/10.4103/aja202453jmjd1ajmjd2ch3k9me2spermatogenesisspermatogonial stem cell |
| spellingShingle | Shan Li Ying Yuan Ke-Yu Zhang Yi-Dan Guo Lu-Tong Wang Xiao-Yuan Zhang Shu Zhang Qi Yan Rong Zhang Jie Chen Feng-Tang Yang Jing-Rui Li A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis Asian Journal of Andrology jmjd1a jmjd2c h3k9me2 spermatogenesis spermatogonial stem cell |
| title | A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| title_full | A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| title_fullStr | A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| title_full_unstemmed | A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| title_short | A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| title_sort | convenient research strategy for functional verification of epigenetic regulators during spermatogenesis |
| topic | jmjd1a jmjd2c h3k9me2 spermatogenesis spermatogonial stem cell |
| url | https://journals.lww.com/10.4103/aja202453 |
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