Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy.
The gradual elimination of chromium from wood preservative formulations results in higher Cu leaching and increased susceptibility to wood decay fungi. Finding a sustainable strategy in wood protection has become of great interest among researchers. The objective of these in vitro studies was to dem...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183004&type=printable |
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| author | Javier Ribera Mónica Gandía Jose F Marcos Maria Del Carmen Bas Siegfried Fink Francis W M R Schwarze |
| author_facet | Javier Ribera Mónica Gandía Jose F Marcos Maria Del Carmen Bas Siegfried Fink Francis W M R Schwarze |
| author_sort | Javier Ribera |
| collection | DOAJ |
| description | The gradual elimination of chromium from wood preservative formulations results in higher Cu leaching and increased susceptibility to wood decay fungi. Finding a sustainable strategy in wood protection has become of great interest among researchers. The objective of these in vitro studies was to demonstrate the effect of T-720-enriched organic charcoal (biochar) against five wood decay basidiomycetes isolated from strongly damaged poles. For this purpose, the antagonistic potential of Trichoderma harzianum (strain T-720) was confirmed among other four Trichoderma spp. against five brown-rot basidiomycetes in dual culture tests. T-720 was genetically transformed and tagged with the green fluorescent protein (GFP) in order to study its antagonistic mechanism against wood decay basidiomycetes. It was also demonstrated that T-720 inhibits the oxalic acid production by basidiomycetes, a well-known mechanism used by brown-rot fungi to detoxify Cu from impregnated wood. Additionally, this study evaluated the effect of biochar, alone or in combination with T-720, on Cu leaching by different preservatives, pH stabilization and prevention of wood decay caused by five basidiomycetes. Addition of biochar resulted in a significant Cu binding released from impregnated wood specimens. T-720-enriched biochar showed a significant reduction of wood decay caused by four basidiomycetes. The addition of T-720-enriched biochar to the soil into which utility poles are placed may improve the efficiency of Cr-free wood preservatives. |
| format | Article |
| id | doaj-art-4ca77b82820e4e68b6ee758892dfccdf |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-4ca77b82820e4e68b6ee758892dfccdf2025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018300410.1371/journal.pone.0183004Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy.Javier RiberaMónica GandíaJose F MarcosMaria Del Carmen BasSiegfried FinkFrancis W M R SchwarzeThe gradual elimination of chromium from wood preservative formulations results in higher Cu leaching and increased susceptibility to wood decay fungi. Finding a sustainable strategy in wood protection has become of great interest among researchers. The objective of these in vitro studies was to demonstrate the effect of T-720-enriched organic charcoal (biochar) against five wood decay basidiomycetes isolated from strongly damaged poles. For this purpose, the antagonistic potential of Trichoderma harzianum (strain T-720) was confirmed among other four Trichoderma spp. against five brown-rot basidiomycetes in dual culture tests. T-720 was genetically transformed and tagged with the green fluorescent protein (GFP) in order to study its antagonistic mechanism against wood decay basidiomycetes. It was also demonstrated that T-720 inhibits the oxalic acid production by basidiomycetes, a well-known mechanism used by brown-rot fungi to detoxify Cu from impregnated wood. Additionally, this study evaluated the effect of biochar, alone or in combination with T-720, on Cu leaching by different preservatives, pH stabilization and prevention of wood decay caused by five basidiomycetes. Addition of biochar resulted in a significant Cu binding released from impregnated wood specimens. T-720-enriched biochar showed a significant reduction of wood decay caused by four basidiomycetes. The addition of T-720-enriched biochar to the soil into which utility poles are placed may improve the efficiency of Cr-free wood preservatives.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183004&type=printable |
| spellingShingle | Javier Ribera Mónica Gandía Jose F Marcos Maria Del Carmen Bas Siegfried Fink Francis W M R Schwarze Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. PLoS ONE |
| title | Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. |
| title_full | Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. |
| title_fullStr | Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. |
| title_full_unstemmed | Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. |
| title_short | Effect of Trichoderma-enriched organic charcoal in the integrated wood protection strategy. |
| title_sort | effect of trichoderma enriched organic charcoal in the integrated wood protection strategy |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183004&type=printable |
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