Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein
In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of w...
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/989543 |
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| author | A. S. Zarena Shubha Gopal R. Vineeth |
| author_facet | A. S. Zarena Shubha Gopal R. Vineeth |
| author_sort | A. S. Zarena |
| collection | DOAJ |
| description | In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is ≈29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 μM, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 μM in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200 μg/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL. |
| format | Article |
| id | doaj-art-4c9ddff2954e403cacbb9c2be04611ea |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-4c9ddff2954e403cacbb9c2be04611ea2025-02-03T01:32:44ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/989543989543Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf ProteinA. S. Zarena0Shubha Gopal1R. Vineeth2Department of Studies in Microbiology, University of Mysore, Mysore 570006, IndiaDepartment of Studies in Microbiology, University of Mysore, Mysore 570006, IndiaDepartment of Studies in Microbiology, University of Mysore, Mysore 570006, IndiaIn the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is ≈29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 μM, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 μM in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200 μg/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL.http://dx.doi.org/10.1155/2014/989543 |
| spellingShingle | A. S. Zarena Shubha Gopal R. Vineeth Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein Journal of Analytical Methods in Chemistry |
| title | Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein |
| title_full | Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein |
| title_fullStr | Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein |
| title_full_unstemmed | Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein |
| title_short | Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein |
| title_sort | antioxidant antibacterial and cytoprotective activity of agathi leaf protein |
| url | http://dx.doi.org/10.1155/2014/989543 |
| work_keys_str_mv | AT aszarena antioxidantantibacterialandcytoprotectiveactivityofagathileafprotein AT shubhagopal antioxidantantibacterialandcytoprotectiveactivityofagathileafprotein AT rvineeth antioxidantantibacterialandcytoprotectiveactivityofagathileafprotein |