Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation

The development of patchouli varieties can increase patchouli oil production in Indonesia. However, the development of varieties in patchouli has been limited because in Indonesia, most of them are cultivated vegetatively, so it is challenging to develop patchouli varieties due to low genetic divers...

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Main Authors: Halida Adistya Putri, Lidia Novita Putri, Aline Sisi Handini
Format: Article
Language:English
Published: Department of Agronomy, Faculty of Agriculture, Universitas Andalas 2025-02-01
Series:JERAMI
Subjects:
Online Access:https://jerami.faperta.unand.ac.id/index.php/Jerami-JIJCS/article/view/186
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author Halida Adistya Putri
Lidia Novita Putri
Aline Sisi Handini
author_facet Halida Adistya Putri
Lidia Novita Putri
Aline Sisi Handini
author_sort Halida Adistya Putri
collection DOAJ
description The development of patchouli varieties can increase patchouli oil production in Indonesia. However, the development of varieties in patchouli has been limited because in Indonesia, most of them are cultivated vegetatively, so it is challenging to develop patchouli varieties due to low genetic diversity. Developing patchouli varieties through gamma-ray irradiation is an effective way to increase the genetic diversity of patchouli plants. Plantlets of the Tapak Tuan variety were used as explants and irradiated with gamma rays at doses of 0, 10, 15, and 20 Gy, with four replications per treatment. After irradiation, shoots were cultured on MS medium, forming first-generation mutant shoots (MV1), which were subcultured for three weeks. These MV1 shoots then developed into second-generation mutant shoots (MV2) through another three-week subculture. This research aims to analyze the morphological diversity of patchouli resulting from gamma-ray irradiation. Data were analyzed using the average value with standard deviation and boxplot. The research showed that an irradiation dose of 10 Gy produced morphological diversity in plant height and number of leaves and nodes
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issn 2654-9395
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publishDate 2025-02-01
publisher Department of Agronomy, Faculty of Agriculture, Universitas Andalas
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spelling doaj-art-4c93a27873a14bf29e45e7a5da3560de2025-08-20T03:11:21ZengDepartment of Agronomy, Faculty of Agriculture, Universitas AndalasJERAMI2654-93952655-30232025-02-0172616610.25077/jijcs.v7i2.186133Increasing the Genetic Diversity of Patchouli through Gamma Ray IrradiationHalida Adistya Putri0Lidia Novita Putri1Aline Sisi Handini2Politeknik Kelapa Sawit Citra Widya EdukasiPoliteknik Kelapa Sawit Citra Widya EdukasiPoliteknik Kelapa Sawit Citra Widya EdukasiThe development of patchouli varieties can increase patchouli oil production in Indonesia. However, the development of varieties in patchouli has been limited because in Indonesia, most of them are cultivated vegetatively, so it is challenging to develop patchouli varieties due to low genetic diversity. Developing patchouli varieties through gamma-ray irradiation is an effective way to increase the genetic diversity of patchouli plants. Plantlets of the Tapak Tuan variety were used as explants and irradiated with gamma rays at doses of 0, 10, 15, and 20 Gy, with four replications per treatment. After irradiation, shoots were cultured on MS medium, forming first-generation mutant shoots (MV1), which were subcultured for three weeks. These MV1 shoots then developed into second-generation mutant shoots (MV2) through another three-week subculture. This research aims to analyze the morphological diversity of patchouli resulting from gamma-ray irradiation. Data were analyzed using the average value with standard deviation and boxplot. The research showed that an irradiation dose of 10 Gy produced morphological diversity in plant height and number of leaves and nodeshttps://jerami.faperta.unand.ac.id/index.php/Jerami-JIJCS/article/view/186essential oilplantletin vitromutationmorphological characters
spellingShingle Halida Adistya Putri
Lidia Novita Putri
Aline Sisi Handini
Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
JERAMI
essential oil
plantlet
in vitro
mutation
morphological characters
title Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
title_full Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
title_fullStr Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
title_full_unstemmed Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
title_short Increasing the Genetic Diversity of Patchouli through Gamma Ray Irradiation
title_sort increasing the genetic diversity of patchouli through gamma ray irradiation
topic essential oil
plantlet
in vitro
mutation
morphological characters
url https://jerami.faperta.unand.ac.id/index.php/Jerami-JIJCS/article/view/186
work_keys_str_mv AT halidaadistyaputri increasingthegeneticdiversityofpatchoulithroughgammarayirradiation
AT lidianovitaputri increasingthegeneticdiversityofpatchoulithroughgammarayirradiation
AT alinesisihandini increasingthegeneticdiversityofpatchoulithroughgammarayirradiation