Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene
We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linking Eimeria acervulina 3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present...
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| Format: | Article |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/654279 |
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| author | Dexing Ma Chunli Ma Mingyang Gao Guangxing Li Ze Niu Xiaodan Huang |
| author_facet | Dexing Ma Chunli Ma Mingyang Gao Guangxing Li Ze Niu Xiaodan Huang |
| author_sort | Dexing Ma |
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| description | We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linking Eimeria acervulina 3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+ cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05, P<0.01, and P<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate for E. acervulina vaccine development. |
| format | Article |
| id | doaj-art-4c92d282ed6c4f90841854c2ce3f3456 |
| institution | OA Journals |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
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| series | Journal of Parasitology Research |
| spelling | doaj-art-4c92d282ed6c4f90841854c2ce3f34562025-08-20T02:38:36ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/654279654279Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 GeneDexing Ma0Chunli Ma1Mingyang Gao2Guangxing Li3Ze Niu4Xiaodan Huang5College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Gongbin Road, Xiangfang District, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Gongbin Road, Xiangfang District, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Gongbin Road, Xiangfang District, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Gongbin Road, Xiangfang District, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Gongbin Road, Xiangfang District, Harbin 150030, ChinaWe previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linking Eimeria acervulina 3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+ cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05, P<0.01, and P<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate for E. acervulina vaccine development.http://dx.doi.org/10.1155/2012/654279 |
| spellingShingle | Dexing Ma Chunli Ma Mingyang Gao Guangxing Li Ze Niu Xiaodan Huang Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene Journal of Parasitology Research |
| title | Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene |
| title_full | Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene |
| title_fullStr | Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene |
| title_full_unstemmed | Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene |
| title_short | Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene |
| title_sort | induction of cellular immune response by dna vaccine coexpressing e acervulina 3 1e gene and mature chil 15 gene |
| url | http://dx.doi.org/10.1155/2012/654279 |
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