The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics
Abstract 17β-estradiol (E2) is an endocrine disruptor, and even trace concentrations (ng/L) of environmental estrogen can interfere with the endocrine system of organisms. Lignin holds promise in enhancing the microbial degradation E2. However, the mechanisms by which lignin facilitates this process...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02605-9 |
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| author | Hanyu Pan Peng Hao Qiannan Li Zongshuo Lv Kun Gao Xiaojun Liang Lianyu Yang Yunhang Gao |
| author_facet | Hanyu Pan Peng Hao Qiannan Li Zongshuo Lv Kun Gao Xiaojun Liang Lianyu Yang Yunhang Gao |
| author_sort | Hanyu Pan |
| collection | DOAJ |
| description | Abstract 17β-estradiol (E2) is an endocrine disruptor, and even trace concentrations (ng/L) of environmental estrogen can interfere with the endocrine system of organisms. Lignin holds promise in enhancing the microbial degradation E2. However, the mechanisms by which lignin facilitates this process remain unclear, which is crucial for understanding complex environmental biodegradation in nature. In this study, we conducted a comprehensive analysis using cellular and lipidomics approaches to investigate the relationship between E2-degrading strain, Rhodococcus sp. RCBS9, and lignin. Our findings demonstrate that lignin significantly enhances E2 degradation efficiency, reaching 94.28% within 5 days with the addition of 0.25 mM lignin. This enhancement is associated with increased microbial growth and activity, reduced of membrane damages, and alleviation of oxidative stress. Fourier Transform Infrared Spectroscopy (FTIR) results indicate that lignin addition alters lipid peaks. Consequently, by analyzing lipid metabolism changes, we further elucidate how lignin addition promotes E2 degradation. |
| format | Article |
| id | doaj-art-4c92b5d4bd8e4c328028f0cbc588fe22 |
| institution | DOAJ |
| issn | 1475-2859 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-4c92b5d4bd8e4c328028f0cbc588fe222025-08-20T02:50:45ZengBMCMicrobial Cell Factories1475-28592024-12-0123111110.1186/s12934-024-02605-9The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomicsHanyu Pan0Peng Hao1Qiannan Li2Zongshuo Lv3Kun Gao4Xiaojun Liang5Lianyu Yang6Yunhang Gao7College of Veterinary Medicine, Jilin Agricultural UniversityCollege of Veterinary Medicine, Jilin Agricultural UniversityCollege of Veterinary Medicine, Jilin Agricultural UniversityCollege of Veterinary Medicine, Jilin Agricultural UniversityCollege of Veterinary Medicine, Jilin Agricultural UniversityInstitute of Animal Husbandry, Ningxia Academy of Agriculture and ForestryCollege of Veterinary Medicine, Jilin Agricultural UniversityCollege of Veterinary Medicine, Jilin Agricultural UniversityAbstract 17β-estradiol (E2) is an endocrine disruptor, and even trace concentrations (ng/L) of environmental estrogen can interfere with the endocrine system of organisms. Lignin holds promise in enhancing the microbial degradation E2. However, the mechanisms by which lignin facilitates this process remain unclear, which is crucial for understanding complex environmental biodegradation in nature. In this study, we conducted a comprehensive analysis using cellular and lipidomics approaches to investigate the relationship between E2-degrading strain, Rhodococcus sp. RCBS9, and lignin. Our findings demonstrate that lignin significantly enhances E2 degradation efficiency, reaching 94.28% within 5 days with the addition of 0.25 mM lignin. This enhancement is associated with increased microbial growth and activity, reduced of membrane damages, and alleviation of oxidative stress. Fourier Transform Infrared Spectroscopy (FTIR) results indicate that lignin addition alters lipid peaks. Consequently, by analyzing lipid metabolism changes, we further elucidate how lignin addition promotes E2 degradation.https://doi.org/10.1186/s12934-024-02605-917β-estradiolLigninBiodegradationCells characteristicsLipidomicsMechanisms of lipid action |
| spellingShingle | Hanyu Pan Peng Hao Qiannan Li Zongshuo Lv Kun Gao Xiaojun Liang Lianyu Yang Yunhang Gao The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics Microbial Cell Factories 17β-estradiol Lignin Biodegradation Cells characteristics Lipidomics Mechanisms of lipid action |
| title | The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics |
| title_full | The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics |
| title_fullStr | The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics |
| title_full_unstemmed | The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics |
| title_short | The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics |
| title_sort | role of lignin in 17β estradiol biodegradation insights from cellular characteristics and lipidomics |
| topic | 17β-estradiol Lignin Biodegradation Cells characteristics Lipidomics Mechanisms of lipid action |
| url | https://doi.org/10.1186/s12934-024-02605-9 |
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