Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway
Our previous study has shown the beneficial effect of the ethanolic extract of Sangyod rice (SE) on lipid accumulation and insulin resistance. However, its effect on vascular inflammation has yet to be explored. The current study aimed to investigate the anti-inflammatory effects of SE in both in vi...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/adpp/1169062 |
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| author | Wanwipha Woonnoi Kornsuda Thipart Wanthanee Hanchang Jirawat Saetan Supita Tanasawet Furoida Moolsup Wiwit Suttithumsatid Tulaporn Wongtawatchai Wanida Sukketsiri |
| author_facet | Wanwipha Woonnoi Kornsuda Thipart Wanthanee Hanchang Jirawat Saetan Supita Tanasawet Furoida Moolsup Wiwit Suttithumsatid Tulaporn Wongtawatchai Wanida Sukketsiri |
| author_sort | Wanwipha Woonnoi |
| collection | DOAJ |
| description | Our previous study has shown the beneficial effect of the ethanolic extract of Sangyod rice (SE) on lipid accumulation and insulin resistance. However, its effect on vascular inflammation has yet to be explored. The current study aimed to investigate the anti-inflammatory effects of SE in both in vitro and in vivo models, specifically examining its impact on LPS-induced inflammation in RAW264.7 cells and evaluating its efficacy in an animal model of diabetes mellitus induced by a high-fat diet combined with a low-dose streptozotocin. In the in vitro experiments, SE treatment effectively suppressed the LPS-induced activation of key signaling pathways, including Akt, ERK1/2, p38 MAPK, and NF-κB, which are known to play pivotal roles in the inflammatory response. SE was also found to reduce oxidative stress and the production of inflammatory markers in the LPS-stimulated RAW264.7 cells. In the in vivo experiments, the administration of SE (500 mg/kg BW) and metformin (200 mg/kg BW) to high-fat diet/streptozotocin-induced diabetic rats effectively improved dyslipidemia, as evidenced by reductions in serum total cholesterol, LDL-cholesterol, and triglycerides compared to the untreated diabetic control group. Importantly, SE ameliorated the damage to the vascular endothelium and elastic fibers by downregulating the expression of proinflammatory cytokines and oxidative stress markers. Additionally, SE administration attenuated the upregulation of key markers associated with ER stress-mediated apoptotic pathways, with effects comparable to those observed in diabetic rats treated with the standard antidiabetic drug metformin. These findings suggest that SE possesses both anti-inflammatory and vascular protective properties, evident in both in vitro and in vivo studies. |
| format | Article |
| id | doaj-art-4c8fe05604f84de9a4501d3260ea4eb0 |
| institution | OA Journals |
| issn | 2633-4690 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-4c8fe05604f84de9a4501d3260ea4eb02025-08-20T02:28:25ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902025-01-01202510.1155/adpp/1169062Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling PathwayWanwipha Woonnoi0Kornsuda Thipart1Wanthanee Hanchang2Jirawat Saetan3Supita Tanasawet4Furoida Moolsup5Wiwit Suttithumsatid6Tulaporn Wongtawatchai7Wanida Sukketsiri8Division of Health and Applied SciencesDivision of Health and Applied SciencesDepartment of PhysiologyDivision of Health and Applied SciencesDivision of Health and Applied SciencesLaboratory Animal Service CenterDepartment of Pharmacognosy and Pharmaceutical BotanyDivision of Health and Applied SciencesDivision of Health and Applied SciencesOur previous study has shown the beneficial effect of the ethanolic extract of Sangyod rice (SE) on lipid accumulation and insulin resistance. However, its effect on vascular inflammation has yet to be explored. The current study aimed to investigate the anti-inflammatory effects of SE in both in vitro and in vivo models, specifically examining its impact on LPS-induced inflammation in RAW264.7 cells and evaluating its efficacy in an animal model of diabetes mellitus induced by a high-fat diet combined with a low-dose streptozotocin. In the in vitro experiments, SE treatment effectively suppressed the LPS-induced activation of key signaling pathways, including Akt, ERK1/2, p38 MAPK, and NF-κB, which are known to play pivotal roles in the inflammatory response. SE was also found to reduce oxidative stress and the production of inflammatory markers in the LPS-stimulated RAW264.7 cells. In the in vivo experiments, the administration of SE (500 mg/kg BW) and metformin (200 mg/kg BW) to high-fat diet/streptozotocin-induced diabetic rats effectively improved dyslipidemia, as evidenced by reductions in serum total cholesterol, LDL-cholesterol, and triglycerides compared to the untreated diabetic control group. Importantly, SE ameliorated the damage to the vascular endothelium and elastic fibers by downregulating the expression of proinflammatory cytokines and oxidative stress markers. Additionally, SE administration attenuated the upregulation of key markers associated with ER stress-mediated apoptotic pathways, with effects comparable to those observed in diabetic rats treated with the standard antidiabetic drug metformin. These findings suggest that SE possesses both anti-inflammatory and vascular protective properties, evident in both in vitro and in vivo studies.http://dx.doi.org/10.1155/adpp/1169062 |
| spellingShingle | Wanwipha Woonnoi Kornsuda Thipart Wanthanee Hanchang Jirawat Saetan Supita Tanasawet Furoida Moolsup Wiwit Suttithumsatid Tulaporn Wongtawatchai Wanida Sukketsiri Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway Advances in Pharmacological and Pharmaceutical Sciences |
| title | Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway |
| title_full | Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway |
| title_fullStr | Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway |
| title_full_unstemmed | Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway |
| title_short | Sangyod Rice Extract Attenuates Vascular Inflammation and Injury in a Rat Model of Diabetes by Modulating the Akt/MAPK Signaling Pathway |
| title_sort | sangyod rice extract attenuates vascular inflammation and injury in a rat model of diabetes by modulating the akt mapk signaling pathway |
| url | http://dx.doi.org/10.1155/adpp/1169062 |
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