Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L.
Given the challenges of climate change, effective adaptation strategies for crops like coffee are crucial. This study evaluated twelve 1-aminocyclopropane-1-carboxylate deaminase-producing bacterial strains selectively isolated from the rhizosphere of <i>Coffea arabica</i> L. cv. Costa R...
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2025-04-01
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| author | Yesenia Jasso-Arreola J. Antonio Ibarra Flor de Fátima Rosas-Cárdenas Paulina Estrada-de los Santos |
| author_facet | Yesenia Jasso-Arreola J. Antonio Ibarra Flor de Fátima Rosas-Cárdenas Paulina Estrada-de los Santos |
| author_sort | Yesenia Jasso-Arreola |
| collection | DOAJ |
| description | Given the challenges of climate change, effective adaptation strategies for crops like coffee are crucial. This study evaluated twelve 1-aminocyclopropane-1-carboxylate deaminase-producing bacterial strains selectively isolated from the rhizosphere of <i>Coffea arabica</i> L. cv. Costa Rica 95 in a plantation located in Veracruz, Mexico, focusing on their potential to enhance drought resistance. The strains, representing seven genera from the Gamma-proteobacteria and Bacteroidota groups, were characterized for growth-promoting traits, including ACC deaminase activity, indole-3-acetic acid (IAA) synthesis, phosphates solubilization, siderophore production, and nitrogen fixation. Strains of the genus <i>Pantoea</i> exhibited higher ACC deaminase activity, phosphate solubilization, and IAA synthesis, while others, such as <i>Sphingobacterium</i> and <i>Chryseobacterium</i>, showed limited plant growth-promoting traits. A pot experiment was conducted with coffee plants subjected to either full irrigation (soil with 85% volumetric water content) or drought (soil with 55% volumetric water content) conditions, along with inoculation with the isolated strains. Plants inoculated with <i>Pantoea</i> sp. RCa62 demonstrated improved growth metrics and physiological traits under drought, including higher leaf area, relative water content (RWC), biomass, and root development compared to uninoculated controls. Similar results were observed with <i>Serratia</i> sp. RCa28 and <i>Pantoea</i> sp. RCa31 under full irrigation conditions. <i>Pantoea</i> sp. RCa62 exhibited superior root development under stress, contributing to overall plant development. Proline accumulation was significantly higher in drought-stressed, non-inoculated plants compared to those inoculated with <i>Pantoea</i> sp. RCa62. This research highlights the potential of <i>Pantoea</i> sp. RCa62 to enhance coffee plant resilience to drought and underscores the need for field application and further validation of these bioinoculants in sustainable agricultural practices. |
| format | Article |
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| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-4c8d9e9056fb4b9bbbc82c213fe8295d2025-08-20T02:15:47ZengMDPI AGPlants2223-77472025-04-01147108410.3390/plants14071084Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L.Yesenia Jasso-Arreola0J. Antonio Ibarra1Flor de Fátima Rosas-Cárdenas2Paulina Estrada-de los Santos3Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, Alcaldía Miguel Hidalgo, Ciudad de México 11340, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, Alcaldía Miguel Hidalgo, Ciudad de México 11340, MexicoInstituto Politécnico Nacional, Centro de Investigaciónen Biotecnología Aplicada, Ex-Hacienda SanJuan Molino Carretera Estatal Tecuexcomac-Tepetitla Km 1.5, Tepetitla 90700, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, Alcaldía Miguel Hidalgo, Ciudad de México 11340, MexicoGiven the challenges of climate change, effective adaptation strategies for crops like coffee are crucial. This study evaluated twelve 1-aminocyclopropane-1-carboxylate deaminase-producing bacterial strains selectively isolated from the rhizosphere of <i>Coffea arabica</i> L. cv. Costa Rica 95 in a plantation located in Veracruz, Mexico, focusing on their potential to enhance drought resistance. The strains, representing seven genera from the Gamma-proteobacteria and Bacteroidota groups, were characterized for growth-promoting traits, including ACC deaminase activity, indole-3-acetic acid (IAA) synthesis, phosphates solubilization, siderophore production, and nitrogen fixation. Strains of the genus <i>Pantoea</i> exhibited higher ACC deaminase activity, phosphate solubilization, and IAA synthesis, while others, such as <i>Sphingobacterium</i> and <i>Chryseobacterium</i>, showed limited plant growth-promoting traits. A pot experiment was conducted with coffee plants subjected to either full irrigation (soil with 85% volumetric water content) or drought (soil with 55% volumetric water content) conditions, along with inoculation with the isolated strains. Plants inoculated with <i>Pantoea</i> sp. RCa62 demonstrated improved growth metrics and physiological traits under drought, including higher leaf area, relative water content (RWC), biomass, and root development compared to uninoculated controls. Similar results were observed with <i>Serratia</i> sp. RCa28 and <i>Pantoea</i> sp. RCa31 under full irrigation conditions. <i>Pantoea</i> sp. RCa62 exhibited superior root development under stress, contributing to overall plant development. Proline accumulation was significantly higher in drought-stressed, non-inoculated plants compared to those inoculated with <i>Pantoea</i> sp. RCa62. This research highlights the potential of <i>Pantoea</i> sp. RCa62 to enhance coffee plant resilience to drought and underscores the need for field application and further validation of these bioinoculants in sustainable agricultural practices.https://www.mdpi.com/2223-7747/14/7/1084drought resistancestress acclimationgrowth promotionrhizobacterial isolation<i>Pantoea</i> |
| spellingShingle | Yesenia Jasso-Arreola J. Antonio Ibarra Flor de Fátima Rosas-Cárdenas Paulina Estrada-de los Santos Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. Plants drought resistance stress acclimation growth promotion rhizobacterial isolation <i>Pantoea</i> |
| title | Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. |
| title_full | Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. |
| title_fullStr | Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. |
| title_full_unstemmed | Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. |
| title_short | Beneficial Effects of ACC Deaminase-Producing Rhizobacteria on the Drought Stress Resistance of <i>Coffea arabica</i> L. |
| title_sort | beneficial effects of acc deaminase producing rhizobacteria on the drought stress resistance of i coffea arabica i l |
| topic | drought resistance stress acclimation growth promotion rhizobacterial isolation <i>Pantoea</i> |
| url | https://www.mdpi.com/2223-7747/14/7/1084 |
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