METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION
Background. Photodynamic Therapy is one of the treatment methods used in modern oncology. Evaluation of the efficacy in vivo of photosensitizers on tumor models is generally accepted, but the photodynamic therapy technique in mice is not without drawbacks.The purpose of the study was evaluation of...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2020-12-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/1643 |
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| author | S. S. Kruglov M. L. Gelfond M. L. Tyndyk M. A. Maydin T. G. Grishacheva R. M. Basina E. A. Gubareva E. A. Plakhov G. S. Kireeva A. V. Panchenko |
| author_facet | S. S. Kruglov M. L. Gelfond M. L. Tyndyk M. A. Maydin T. G. Grishacheva R. M. Basina E. A. Gubareva E. A. Plakhov G. S. Kireeva A. V. Panchenko |
| author_sort | S. S. Kruglov |
| collection | DOAJ |
| description | Background. Photodynamic Therapy is one of the treatment methods used in modern oncology. Evaluation of the efficacy in vivo of photosensitizers on tumor models is generally accepted, but the photodynamic therapy technique in mice is not without drawbacks.The purpose of the study was evaluation of the efficacy of photodynamic therapy in mice with Ehrlich tumor model after subcutaneous and intracutaneous injection of tumor cells.Material and Methods. The study was conducted on BAL B/C mice of both sexes. Fotoditazin® and Radachlorin® were used as photosensitizers. For photoactivation, the Alod laser apparatus with a wavelength of 662 nm was used.Results. A comparison of photodynamic therapy with subcutaneous and intracutaneous localization of Ehrlich tumor was performed. It was shown that depending on the location and depth of inoculation of Ehrlich tumor, the pharmacokinetics (both the fluorescence intensity over time and the contrast ratio of the tumor/surrounding tissue) and pharmacodynamics (tumor growth inhibition, survival) of photosensitizers are significantly different. Higher contrast of the tumor/surrounding tissue is observed with intracutaneous localization of the tumor.Conclusion. A model with intracutaneous localization of Ehrlich tumor can be recommended for a primary assessment of efficacy; it allows the use of fewer animals in the experiment. When planning experiments to study photosensitizers and evaluating their results, the advantages and disadvantages of different methods for modeling tumors in mice should be taken into account. |
| format | Article |
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| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2020-12-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-4c80f6dfd643458e9764a48ad47000a82025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682020-12-01196829210.21294/1814-4861-2020-19-6-82-92808METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATIONS. S. Kruglov0M. L. Gelfond1M. L. Tyndyk2M. A. Maydin3T. G. Grishacheva4R. M. Basina5E. A. Gubareva6E. A. Plakhov7G. S. Kireeva8A. V. Panchenko9FSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFPavlov First Saint Petersburg State Medical UniversitySt. Petersburg State Pediatric Medical University of the Ministry of Healthcare of RussiaLaboratory for Carcinogenesis and Aging, FSBI «N.N. Petrov NMRC of Oncology» of Ministry of Healthcare of RussiaFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFFSBI «N.N. Petrov National Medical Research Centre of Oncology» Ministry of Public Health of RFBackground. Photodynamic Therapy is one of the treatment methods used in modern oncology. Evaluation of the efficacy in vivo of photosensitizers on tumor models is generally accepted, but the photodynamic therapy technique in mice is not without drawbacks.The purpose of the study was evaluation of the efficacy of photodynamic therapy in mice with Ehrlich tumor model after subcutaneous and intracutaneous injection of tumor cells.Material and Methods. The study was conducted on BAL B/C mice of both sexes. Fotoditazin® and Radachlorin® were used as photosensitizers. For photoactivation, the Alod laser apparatus with a wavelength of 662 nm was used.Results. A comparison of photodynamic therapy with subcutaneous and intracutaneous localization of Ehrlich tumor was performed. It was shown that depending on the location and depth of inoculation of Ehrlich tumor, the pharmacokinetics (both the fluorescence intensity over time and the contrast ratio of the tumor/surrounding tissue) and pharmacodynamics (tumor growth inhibition, survival) of photosensitizers are significantly different. Higher contrast of the tumor/surrounding tissue is observed with intracutaneous localization of the tumor.Conclusion. A model with intracutaneous localization of Ehrlich tumor can be recommended for a primary assessment of efficacy; it allows the use of fewer animals in the experiment. When planning experiments to study photosensitizers and evaluating their results, the advantages and disadvantages of different methods for modeling tumors in mice should be taken into account.https://www.siboncoj.ru/jour/article/view/1643photodynamic therapyehrlich tumorphotosensitizermodelfotoditazinradachlorinmice |
| spellingShingle | S. S. Kruglov M. L. Gelfond M. L. Tyndyk M. A. Maydin T. G. Grishacheva R. M. Basina E. A. Gubareva E. A. Plakhov G. S. Kireeva A. V. Panchenko METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION Сибирский онкологический журнал photodynamic therapy ehrlich tumor photosensitizer model fotoditazin radachlorin mice |
| title | METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION |
| title_full | METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION |
| title_fullStr | METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION |
| title_full_unstemmed | METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION |
| title_short | METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION |
| title_sort | methodological aspects of photodynamic therapy of ehrlich solid carcinoma in balb c mouse strain with various tumor localization |
| topic | photodynamic therapy ehrlich tumor photosensitizer model fotoditazin radachlorin mice |
| url | https://www.siboncoj.ru/jour/article/view/1643 |
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